Prevalence, Virulence Gene Profiling, and Characterization of Enteroaggregative Escherichia coli from Children with Acute Diarrhea, Asymptomatic Nourished, and Malnourished Children Younger Than 5 Years of Age in India

Enteroaggregative Escherichia coli as etiological agent of endemic diarrhea in Spain: A prospective multicenter prevalence study with molecular characterization of isolates

“…In particular, Pabst et al (2003) and Cohen et al (2005) detected EAEC from children ≤5 years with diarrhea significantly more frequently than from healthy children (11.9% vs. 2.2 and 9.2% vs. 3.3%, respectively), although the association of EAEC with diarrhea did not achieve statistical significance in our case–control study. On the contrary, as expected, our detection rates are substantially lower than those generally reported in developing countries, with EAEC prevalences up to 39% in this setting ( Gonzalez et al, 1997 ; Okeke et al, 2000 ; Modgil et al, 2021 ; Manhique-Coutinho et al, 2022 ).…”

“…In particular, Pabst et al (2003) and Cohen et al (2005) detected EAEC from children ≤5 years with diarrhea significantly more frequently than from healthy children (11.9% vs. 2.2 and 9.2% vs. 3.3%, respectively), although the association of EAEC with diarrhea did not achieve statistical significance in our case-control study. On the contrary, as expected, our detection rates are substantially lower than those generally reported in developing countries, with EAEC prevalences up to 39% in this setting (Gonzalez et al, 1997;Okeke et al, 2000;Modgil et al, 2021;Manhique-Coutinho et al, 2022). Although there were 26 different serogenotypes among the 120 isolates, 82% were restricted to only nine serogenotypes, very homogeneous with respect to ST, with some particularly common serogenotype-ST combinations, such as O126:H27-ST200, O111:H21-ST40, and O92:H33-ST34, comprising 50% of isolates.…”

First Report of Enteroaggregative E. Coli Harbouring NDM-4 and NDM-7 From Asymptomatic Children in India

“…Here for the rst time, we report EAEC harbouring NDM-4 and NDM-7 variants isolated from healthy children. We collected stool samples from 550 healthy children <5 years of age from 25 anganwadi centers of Chandigarh which offer primary healthcare to children as a part of the government public healthcare system (9). The EAEC were identi ed by using pCVD432 gene coding for CVD432 on pAA plasmid of EAEC (9).…”

“…We collected stool samples from 550 healthy children <5 years of age from 25 anganwadi centers of Chandigarh which offer primary healthcare to children as a part of the government public healthcare system (9). The EAEC were identi ed by using pCVD432 gene coding for CVD432 on pAA plasmid of EAEC (9). Whole genomes of EAEC strains (n= 33) were sequenced on Illumina Hiseq platform to generate 150 x 2 bp paired end reads and the sequences were analysed for in silico MLST, serotyping, identi cation of antimicrobial resistance (AMR) genes, plasmid replicons by SRST2 v0.2.0 (10).…”

Analysis of the virulence and inflammatory markers elicited by Enteroaggregative Escherichia coli isolated from clinical and non-clinical sources in an experimental infection model, India

“…Diarrhoeal illnesses are a major cause of childhood mortality worldwide. Among different pathotypes of diarrheagenic Escherichia coli (DEC), Enteroaggregative E. coli (EAEC) is gaining importance as it causes persistent and acute diarrhea both in developing and developed countries [1][2][3].EAEC is increasingly recognized as a common cause of diarrhea in healthy, malnourished, and immune-deficient adults and children [4][5][6][7]. The pathogenesis of EAEC is not completely understood.…”