Prevalence, species differentiation, haemolytic activity, and antibiotic susceptibility of aeromonads in untreated well water

Ghenghesh, Khalifa Sifaw; El-Ghodban, Abdelmula; Dkakni, Rabia; Abeid, Salaheddin; Altomi, A. S.; Abdussalam, Tarhuni; Màrialigeti, Károly

doi:10.1590/s0074-02762001000200006

Cited by 19 publications

(21 citation statements)

References 35 publications

(23 reference statements)

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“…Despite the fact that 19 named species have been classified, only few of them are recognized as human pathogens, such as A. hydrophila complex, A. caviae and A. veronii, representing almost 85% of the clinical isolates, although other species have been recovered from the clinical sources (Janda and Abbot, 1998). Aeromonads are also found in a variety of sources, such as the food (Granum et al, 1998;Cansian et al, 2005;Ullmann et al, 2005;Martin Talavea et al, 2006), natural water (Ghenghesh et al, 2001;Østensvik, 2001, Sousa andSilva-Souza 2001) and drinking water (Kuhn et al, 1997;Villari et al, 2003), and it is known that psycrophilic species are mainly etiologic agents in the fishes (Garduño et al, 2000). In human immunocompetent hosts, the members of this genus may be responsible for the intense gastroenteritis, called traveler's diarrhea (Janda and Abbot, 1998;Vila et al, 2003), but in the immunocompromised hosts, these bacteria could be implicated in the cases of myonecrosis, bacteremia, septicaemia (Martino et al, 1997) and hepatic cirrhosis and neoplasys (Chien Ko and Chuang, 1995).…”

Section: Introductionmentioning

confidence: 99%

The occurrence of Aeromonas spp. in the bottled mineral water, well water and tap water from the municipal supplies

Scoaris

Bizerra

Yamada‐Ogatta

et al. 2008

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 99%

The occurrence of Aeromonas spp. in the bottled mineral water, well water and tap water from the municipal supplies

Scoaris

Bizerra

Yamada‐Ogatta

et al. 2008

Braz. arch. biol. technol.

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“…Interpretation of the results was made by referring to the identification table and analytical profile index after 18 h of incubation at 37°C. Resistance to vibriostatic agent, 2,4-diamino-6,7-diisopropylpteridine (0/129), was performed as detailed previously (5). For precise identification, 16S ribosomal DNA (rDNA) sequencing of the strains was done using the MicroSeq 500-16S rDNA bacterial sequencing kit (PE Applied Biosystems).…”

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confidence: 99%

“…from aquatic sources in Calcutta, India, were found to Since both H. pylori and Aeromonas spp. are known to survive in raw water and since both are human enteric pathogens (3,5,10), such a possibility could not be ruled out.…”

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confidence: 99%

Environmental Isolates of Aeromonas spp. Harboring the cagA -Like Gene of Helicobacter pylori

Datta

Khan

Nandy

et al. 2003

Appl Environ Microbiol

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We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.Helicobacter pylori is one of the most genetically diverse bacterial species as seen by DNA fingerprinting and in tests of gene content and chromosomal gene order (6). It has been suggested that considerable gene transfer from disparate phylogenetic groups into the H. pylori lineage shaped the gene pool of this organism during its evolution (1). Support for this is provided by (i) the abundance of putative restriction and modification genes, many of which are most closely related to those of other bacterial species; (ii) the significant sequence similarity of many predicted H. pylori proteins to those from different major taxonomic groups (eukaryotes, Archaea, and gram-positive bacteria), and (iii) the presence of transposable elements IS605 to IS608 (1,7,8,9). However, it is not known how ancient these gene acquisitions are: in particular, which preceded and which followed the emergence of H. pylori as a species or the nature of transposition and recombination events that led to their acquisition. In fact, this evidence of interspecific gene transfer poses interesting new challenges for attempts to reconstruct the evolutionary history of the organism.The cag pathogenicity island (cag PAI) is a well-known virulence marker of H. pylori (2) that is believed to be acquired initially by horizontal gene transfer from an unrelated species and over its evolution has adopted various intermediate types by chromosomal rearrangement mediated by direct repeats and IS605 (1). cag PAI is a 40-kb DNA segment with 31 predicted open reading frames, of which the gene cagA, localized to the 3Ј end of the island, encodes an immunodominant antigen, CagA, often used as a marker of cag PAI (13). At present, however, nothing is known about the evolution of cag PAI or how it was acquired by H. pylori. It is often found that the virulence genes are dispersed among the environmental strains of the same or distant species and can be ferried about, given the fact that most of them are located on mobile elements. Thus, it is possible that there may be an environmental reservoir of the genes of cag PAI that needs to be investigated. This prompted us to conduct a study for determining the prevalence of cagA in the aquatic microflora.During September 2000 to March 2001, water samples from four freshwater ponds and a freshwater lake of Calcutta, India, were collected (5 cm below the water surface) in presterilized glass bottles and processed in the laboratory within 2 to 3 h of collection. One hundred ...

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“…These waters are used for leisure activities and they are also used for oyster fishing, which is normally eaten raw. Generally, Aeromonas spp are the most commonly found contaminants in fish and marine products, which to an extent is explained by their ubiquitous nature in aquatic environments Hãnninen et al (10). In rivers, they form part of the normal microbiota being able to multiply under normal environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Aeromonas species isolated from an estuarine environment

Evangelista-Barreto¹,

Carvalho²,

Vieira³

et al. 2010

Braz. J. Microbiol.

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Thirty water samples were collected, at two week intervals, from the estuary of the River Cocó. The aim was to characterize the presence, distribution and types of Aeromonas spp, in the estuary of the River caviae, A. media, A. sóbria and A. veronii bv. sobria. Aeromonas caviae showed the highest multiple resistance, being resistant to four antibiotics. The presence of those microorganisms may contribute to the occurrence of gastroenteritis, mainly in children, since they are considered opportunists.

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Prevalence, species differentiation, haemolytic activity, and antibiotic susceptibility of aeromonads in untreated well water

Cited by 19 publications

References 35 publications

The occurrence of Aeromonas spp. in the bottled mineral water, well water and tap water from the municipal supplies

The occurrence of Aeromonas spp. in the bottled mineral water, well water and tap water from the municipal supplies

Environmental Isolates of Aeromonas spp. Harboring the cagA -Like Gene of Helicobacter pylori

Characterization of Aeromonas species isolated from an estuarine environment

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