Prevalence, Serotype Identification by Multiplex Polymerase Chain Reaction and Antimicrobial Resistance Patterns of Listeria Monocytogenes Isolated from Retail Foods

Abstract

In the present study, a total of 512 food samples composed of raw milk, dairy products, meat/meat products, chicken meat, seafood and raw vegetables were analyzed for the presence of Listeria monocytogenes. The results of the standard identification methods showed that 20 (3.9%) of the analyzed samples were found to harbor this pathogen. Further, 8.4% (13/155) of chicken meats, 0.9% (1/105) of meat/meat products and 13.6% (6/44) of fresh vegetables were contaminated with L. monocytogenes. Interestingly, only 18 of these isolates gave expected band size when they were subjected to molecular confirmation by polymerase chain reaction (PCR). Multiplex PCR serotyping of the strains revealed that 66.6% (12/18) of which belonged to serotype 1/2a (or 3a), 5.6% (1/18) to serotype 1/2b (or 3b, 7), 5.6% (1/18) to serotype 1/2c (or 3c) and 11.1% (2/18) to serotype 4b (or 4d, 4e). Two strains could not be serotyped by multiplex PCR. The strains were also evaluated by disk diffusion assay for their susceptibility to 15 commonly used antimicrobials. Antimicrobial resistance was most frequently observed for clindamycin (94.4%), followed by streptomycin and kanamycin (88.9%); penicillin (72.2%), tetracycline and gentamicin (66.7%); quinopristin/dalfopristin and erythromycin (61.1). Interestingly, 13 strains were resistant to more than five antibiotics. All strains were susceptible to linezolid, teicoplanin and vancomycin. Practical Applications Listeria monocytogenes is one of the most important foodborne pathogens responsible for several outbreaks and cases of listeriosis in human. This study focused on the prevalence of L. monocytogenes in different raw and ready‐to‐eat foodstuffs, and serotype distribution among the isolates. Antibiotic resistance profiles of the isolates were also reported. Information and relief provided to consumers could help elaborate public health and food safety.

First Report of Listeria monocytogenes Serotypes Detected from Milk and Milk Products in Sri Lanka

“…It was found that serotype 1/2b (or 3b) was rare with only one positive in raw milk and two positives being picked up in pasteurized milk. This is in line with most studies that have found serotypes 1/2a and 1/2b was to be the most common serotypes in food (Aarnisalo et al, 2003: Wallace et al, 2003: Cetinkaya et al 2014.…”

Characterization of Listeria monocytogenes from three countries and antibiotic resistance differences among countries and Listeria monocytogenes serogroups

“…Cetinkaya et al . () further reported that 65% of the isolates were resistant to more than five antibiotics. However, lower multidrug resistance percentages were reported by other researchers (Rodas‐Suárez et al .…”

“…; Cetinkaya et al . ). But, the levels of resistance seem to vary by geographical locations and antimicrobial usage in humans and animals.…”

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

“…After assessing all the information from the recovered publications, seventy-eight primary studies [6,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,…”