Prevalence, serodiversity and antibiogram of enterotoxigenic Escherichia coli (ETEC) in diarrhoeic calves and lambs of Kashmir valley (J&amp;K), India

Manzoor, Rusheeba; Shah, Mohammad; ul-husna, Asma; Wani, S.A.; Pandit, Farhat; Dar, Pervaiz Ahmad; Mir, Mohammad Iqbal

doi:10.31018/jans.v7i1.635

Cited by 8 publications

(8 citation statements)

References 11 publications

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“…In this study, enterotoxigenic E. coli (ETEC) isolates represented 12.7% of isolated E. coli with a similar rate between cattle and buffalo calves. Comparable results were previously recorded the percentage rate of ETEC as 10 to 11% (Güler Borriello et al (2012) in Italy, and Manzoor et al (2015) in India.…”

Section: Discussionsupporting

confidence: 80%

“…This result was correlated with Borriello et al (2012) who detected only the lt gene in ETEC isolates from water buffalo calves in Italy. The absence of lt gene in cattle calves was mentioned in several studies by many authors (Nagy and Fekete 2005;Osman et al 2013;Manzoor et al 2015). The lack of LT toxin in cattle calves was not unexpected as LT toxin is assumed to be an atypical gene in bovine ETEC isolates (Woodward and Wray 1990).…”

Section: Discussionmentioning

confidence: 88%

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Molecular characterization of pathogenic Escherichia coli isolated from diarrheic and in-contact cattle and buffalo calves

Awad

El-Sayed

Mohammed

et al. 2020

Trop Anim Health Prod

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Escherichia coli field isolates from calves were characterized and categorized into the most significant diarrheagenic pathotypes using polymerase chain reaction (PCR) assays with different specific primers. The used PCR systems were designed to detect sequences representing the group-specific virulence genes encoding fimbriae f5 (K99), Shiga toxins (stx 1 and stx 2 ), heat-stable enterotoxins (st), heat-labile enterotoxins (lt), intimin (eae), hemolysin (hylA), and EAEC heat-stable enterotoxin (astA). In the present work, a total of 150 E. coli field isolates were recovered from 150 fecal swabs collected from 100 diarrheic and 50 apparently healthy in-contact cattle and buffalo calves under 3 months old. Out of these 150 isolated E. coli, 106 isolates from 77 diarrheic and 29 in-contact calves harbored one or more of the investigated virulence genes. The pathotyping of the isolates could classify them into shigatoxigenic E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 88%

Molecular characterization of pathogenic Escherichia coli isolated from diarrheic and in-contact cattle and buffalo calves

Awad

El-Sayed

Mohammed

et al. 2020

Trop Anim Health Prod

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“…Andrade et al (2012) detected virulence genes characteristic of this pathotype in both healthy calves and animals with diarrhea. In a study with calves in the Kashmir Valley, India, Manzoor et al (2015) diagnosed ETEC in 15% of the 200 evaluated animals.…”

Section: Resultsmentioning

confidence: 99%

First detection of diarrheagenic Escherichia coli pathotypes in the Mearim River Watershed, Maranhão State, Brazil

Monteiro¹,

Mendes²,

Menezes³

et al. 2021

Semina: Ciênc. Agrár.

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The Mearim River Watershed has multiple uses e.g. leisure, navigation, fishing and subsistence agriculture and constitutes the main source of supply for the populations of municipalities situated along its course. In addition to being a water supply source, the existence of the ‘pororoca’ (tidal bore) effect in a stretch of the lower course of the Mearim River attracts people from several Brazilian states and different countries, as it offers excellent conditions for surfing in fresh water. In this respect, given the importance of the watershed, this study was developed to report the detection of diarrheagenic Escherichia coli pathotypes in a stretch of the lower course of the Mearim River, located in the state of Maranhão, Brazil. Thirty water samples were collected from 10 sampling points. To quantify E. coli, the chromogenic enzymatic system was used and positive samples were isolated and biochemically identified. Pure cultures underwent DNA extraction by heating followed by polymerase chain reaction (PCR) characterization. At the time of the collections, an observation schedule was used to record information on the existence of rearing of livestock and domestic animals; businesses; residences; and fruit and vegetable farming on the riverbanks. The samples were analyzed for the mean populations of E. coli, which ranged from 444 to 2,585 MPN mL-1. Twenty bacterial isolates were identified and the diarrheal pathotypes ETEC, typical EPEC and atypical EPEC were detected. The detection of these pathotypes can represent an epidemiological risk and compromise several uses of this water resource, such as irrigation of fruits and vegetables consumed raw, fishing, animal watering and recreation. Structural investments in basic sanitation are essential to minimize environmental degradation resulting from anthropic activities and to act preventively in public health. In addition, the recovery of riparian forests along the watershed and the maintenance of vegetation in these areas are measures to reduce the transport of particles from the soil to the watercourses, improving the qualitative and quantitative characteristics of this water resource.

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“…Enterotoxigenic E. coli infections usually occur in animals less than five days old, so this bacterial group may have participated in the development of diarrhea observed in calves. Most strains of E. coli present in the gastrointestinal tract are non-pathogenic commensals and participate in the normal process of colonization of the intestine (Manzoor et al 2015, El-Seedy et al 2016). Unfortunately we did not perform E. coli research and identify virulence factors or search for genes that encode toxins (Andrade et al 2012, Costa et al 2014.…”

Section: Discussionmentioning

confidence: 99%

Influence of early use of antimicrobial on the health and performance of Holstein calves in the first month of life

et al. 2020

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The early use of antimicrobial therapy has been introduced in many farms to prevent diarrhea and respiratory disease in young calves; however, there is controversy about whether this practice has a beneficial effect on the health of these animals. This study evaluated the influence of the early use of antimicrobials on the health and performance of neonatal Holstein calves. Twenty-six Holstein calves were screened and divided into two groups, according to the administration (ATB+), or not (ATB-) of tulathromycin (2.5mg/kg, subcutaneously) within the first 12 hours of life. Calves were evaluated by general clinical examination, fecal score, respiratory score, and external palpation of the umbilical region, besides fecal output of dry matter. Anemia was determined by using an automatic system and, also, using a commercial kit for iron dosage. Diarrhea was diagnosed by a centrifuge-flotation technique using a sugar solution (Cryptosporidium) and multiplex semi-nested RT-PCR (rotavirus/coronavirus). The performance of the calves was estimated by Daily Weight Gain (DWG). The young dairy calves were evaluated within 12 hours of birth (≤12h) and at 3-5th (D3-5), 7-9th (D7-9), 13-15th (D13-15), 20-23rd (D20-23), and 27-30th (D27-30) days of life. No difference was noted between the ATB+ and ATB- groups concerning heart rate, respiratory frequency, and rectal temperature. Erythrogram showed a higher frequency of anemia in ATB- group (P=0.016) at the D3-5 check-up; lower values of serum iron were also observed simultaneously (P=0.051). Thirteen cases of respiratory disease were detected during this study; however, no significant difference was observed between the groups in this regard. The frequency of diarrhea (fecal score 2-3) was high in both groups, peaking at D13-D15. No differences were noted between the groups regarding the frequency of diarrhea when considering the dry fecal matter. The predominant etiological agent for diarrhea was Cryptosporidium spp.. The DWG was similar between groups, with maximum weight reduction on D13-15. The administration of tulathromycin in prophylactic dose (2.5mg/kg) at birth decreased the frequency of anemia but did not influence weight gain or the prevalence of diarrhea.

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Prevalence, serodiversity and antibiogram of enterotoxigenic Escherichia coli (ETEC) in diarrhoeic calves and lambs of Kashmir valley (J&K), India

Cited by 8 publications

References 11 publications

Molecular characterization of pathogenic Escherichia coli isolated from diarrheic and in-contact cattle and buffalo calves

Molecular characterization of pathogenic Escherichia coli isolated from diarrheic and in-contact cattle and buffalo calves

First detection of diarrheagenic Escherichia coli pathotypes in the Mearim River Watershed, Maranhão State, Brazil

Influence of early use of antimicrobial on the health and performance of Holstein calves in the first month of life

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