Prevalence, risk factors and molecular characteristics of Shiga toxin-producing Escherichia coli in beef abattoirs in Gauteng, South Africa

Onyeka, Libby O.; Adesiyun, Abiodun A.; Keddy, Karen H.; Manqele, Ayanda; Madoroba, Evelyn; Thompson, Peter

doi:10.1016/j.foodcont.2020.107746

Cited by 5 publications

(7 citation statements)

References 61 publications

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“…in a survey conducted in abattoirs in Jos, Nigeria, isolated L. monocytogenes (2.5%), L. ivanovii (33.6%), L. grayi (4.4%), and L. seeligeri (1.7%). The overall prevalence of L. monocytogenes (4.6%) in the samples collected from the abattoirs in the current study is lower than reported for beef abattoirs in other countries(Takahashi et al 2007;Demaître et al 2021;Al et al 2022), ndings which may be due to differences in the prevalence of L. monocytogenes in slaughtered cattle from the farms and sanitary practices during slaughter which affect cross-contamination of carcasses by L. monocytogenes and other pathogens(Onyeka et al 2021). Contrary to our failure to detect any signi cant effect of the three variables (area, type of abattoir, and kind of sample) on the prevalence of L. monocytogenes, others have documented the impact of the regional location of abattoirs(Demaître et al 2021), type of abattoirs, HT versus LT(Onyeka et al 2021), and types of samples(Matle et al 2019;Dunka et al 2021).…”

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confidence: 66%

“…On the cattle farms consisting of feedlots (n = 3), cow-calf operations (n = 10), and communal farms (n = 10) in Gauteng, South Africa, the following samples were aseptically collected as described by Onyeka et al (2021). Faecal or freshly voided faeces of individual cattle, pooled faeces from areas where cattle congregated, drinking water in troughs, feeds in feeding troughs, and silage.…”

Section: Cattle Farmsmentioning

confidence: 99%

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A comparative study on the occurrence, risk factors, and genetic characteristics of Listeria species recovered from cattle farms, and beef abattoirs in Gauteng Province, South Africa

Gana

Gcebe

Moerane

et al. 2023

Preprint

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These cross-sectional reported the occurrence, risk factors, and genetic characteristics of Listeria species recovered from cattle farms and beef abattoirs in Gauteng Province, South Africa. A total of 328 samples collected from 23 cattle farms and 262 samples from 8 beef abattoirs were processed using standard bacteriological and molecular methods to detect Listeria spp. The factors associated with the prevalence of Listeria spp. were investigated, and multiplex polymerase chain reaction (mPCR) was used to determine Listeria species, the pathogenic serogroups, and carriage of eight virulence-associated genes by Listeria monocytogenes. The overall prevalence of Listeria spp. in cattle farms was 14.6%, comprising Listeria innocua (11.3%), Listeria monocytogenes (3.4%), Listeria welshimeri (0.0%) compared with 11.1%, comprising Listeria innocua (5.7%), Listeria monocytogenes (4.6%), Listeria welshimeri (0.8%) for beef abattoirs. Of the three variables (area, type of farm/abattoir, and sample type) investigated, only the sample types at abattoirs had a significant (P < 0.001) effect on the prevalence of L. innocua and L. welshimeri. The frequency of distribution of the serogroups based on 11 L. monocytogenes isolated from farms was 72.7% and 27.3% for the serogroup 1/2a-3a and 4b-4d-4e, respectively, while for the 12 L. monocytogenes isolates recovered from abattoirs, it was 25%, 8.3%, 50% and 16.7% for the serogroup 1/2a-3a, 1/2b-3b, 1/2c-3c, and 4b-4d-4e respectively (P < 0.05). All (100.0%) isolates of L. monocytogenes from the farms and abattoirs were positive for seven virulence genes (hlyA, inlB, plcA, iap, inlA, inlC, and inlJ). The clinical and food safety significance of the findings cannot be ignored.

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contrasting

confidence: 66%

Section: Cattle Farmsmentioning

confidence: 99%

A comparative study on the occurrence, risk factors, and genetic characteristics of Listeria species recovered from cattle farms, and beef abattoirs in Gauteng Province, South Africa

Gana

Gcebe

Moerane

et al. 2023

Preprint

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“…The differences in the types and frequencies of Listeria species detected in abattoirs across countries may be partly due to the prevalence of L. monocytogenes in slaughtered cattle and sanitary practices during slaughter, which affect cross-contamination of carcasses by L. monocytogenes and other pathogens (Demaître et al 2020 ; 2021 ; Mpundu et al 2022a ; Onyeka et al 2021 ). Contrary to the lack of any significant effect of the three variables on the prevalence of L. monocytogene s in our study, others have documented the impact of the regional location of abattoirs (Demaître et al 2021 ), type of abattoirs, HT versus LT (Onyeka et al 2021 ), and types of samples (Dunka et al 2021 ; Matle et al 2019 ) on the prevalence of L. monocytogenes.…”

Section: Discussionmentioning

confidence: 99%

“…For the cattle farms consisting of feedlots ( n = 3), cow-calf operations ( n = 10), and communal farms ( n = 10) in Gauteng, South Africa, samples were aseptically collected as described by Onyeka et al ( 2021 ). Faecal (rectal faecal grab or freshly voided faeces) of individual cattle, and environmental samples inclusive of pooled faeces from areas where cattle congregated, drinking water in troughs, feeds (grains and grass), and silage in feeding troughs.…”

Section: Methodsmentioning

confidence: 99%

A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa

Gana,

Gcebe,

Moerane

et al. 2024

Trop Anim Health Prod

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These cross-sectional studies reported the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa. A total of 328 samples (faeces, feeds, silage, and drinking water) were collected from 23 cattle farms (communal, cow-calf, and feedlot), and 262 samples (faeces, carcass swabs, and effluents) from 8 beef abattoirs (low throughput and high throughput) were processed using standard bacteriological and molecular methods to detect Listeria species. The factors associated with the prevalence of Listeria species were investigated, and multiplex polymerase chain reaction (mPCR) was used to determine Listeria species, the pathogenic serogroups, and the carriage of eight virulence-associated genes by Listeria monocytogenes. The overall prevalence of Listeria species in cattle farms was 14.6%, comprising Listeria innocua (11.3%), Listeria monocytogenes (3.4%), Listeria welshimeri (0.0%) compared with 11.1%, comprising Listeria innocua (5.7%), Listeria monocytogenes (4.6%), Listeria welshimeri (0.8%) for beef abattoirs. Of the three variables (area, type of farm/abattoir, and sample type) investigated, only the sample types at abattoirs had a significant (P < 0.001) effect on the prevalence of L. innocua and L. welshimeri. The frequency of distribution of the serogroups based on 11 L. monocytogenes isolated from farms was 72.7% and 27.3% for the serogroup 1/2a-3a and 4b-4d-4e, respectively, while for the 12 L. monocytogenes isolates recovered from abattoirs, it was 25%, 8.3%, 50% and 16.7% for the serogroup 1/2a-3a, 1/2b-3b, 1/2c-3c, and 4b-4d-4e respectively (P < 0.05). All (100%) isolates of L. monocytogenes from the farms and abattoirs were positive for seven virulence genes (hlyA, inlB, plcA, iap, inlA, inlC, and inlJ). The clinical and food safety significance of the findings cannot be ignored.

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“…On the other hand, other scholars reported higher results than the present findings such as 13.3% in Addis Ababa (Tizeta et al, 2014) from Ethiopia. In Africa, 35.1% of occurrences were reported in South Africa (Onyeka et al, 2021).…”

Section: Occurrence Of E Coli O157:h7mentioning

confidence: 99%

Escherichia coli O157:H7 beef carcass contamination and its antibiotic resistance in Awi Zone, Northwest Ethiopia

Tarekegn,

Mitiku,

Alemu

2023

Food Science & Nutrition

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Escherichia coli O157:H7 is a cause of foodborne disease and global public health issues especially in developing countries like Ethiopia. A cross‐sectional study was done from January 2022 to July 2022 in Awi Zone to assess the occurrence and antibiograms of E. coli O157:H7. Abattoirs and butcher shops were selected purposively, whereas a systematic random and purposive sampling technique was employed to select study units in abattoirs and butcher shops, respectively. A total of 248 swab samples were collected, isolated, and confirmed using bacteriological culture, biochemical tests, and latex agglutination tests. Escherichia coli O1157:H7 antibiogram tests were performed using Kirby–Bauer disk diffusion method. Logistic regression was used to analyze and measure the degree of association between the presumed risk factors and E. coli O157:H7 occurrence. The overall occurrence of E. coli O157:H7 was estimated to be 8.87% and a relative higher (11.29%) occurrence of E. coli O157:H7 was recorded at butcher shops when compared to abattoirs (6.45%). All isolates were susceptible to gentamicin followed by chloramphenicol (81.81%). About 81.81% of the isolates were resistant to ampicillin and 77.23% of isolates developed resistance to two and more than two antibiotics (MDR). In conclusion, E. coli O157:H7 was detected in the study area. Thus, educating abattoir and butcher shop workers, and consumers, on hygienic handling practices and safe consumption of meat could eliminate foodborne infection associated with E. coli O157:H7 occurrence.

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Prevalence, risk factors and molecular characteristics of Shiga toxin-producing Escherichia coli in beef abattoirs in Gauteng, South Africa

Cited by 5 publications

References 61 publications

A comparative study on the occurrence, risk factors, and genetic characteristics of Listeria species recovered from cattle farms, and beef abattoirs in Gauteng Province, South Africa

A comparative study on the occurrence, risk factors, and genetic characteristics of Listeria species recovered from cattle farms, and beef abattoirs in Gauteng Province, South Africa

A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa

Escherichia coli O157:H7 beef carcass contamination and its antibiotic resistance in Awi Zone, Northwest Ethiopia

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