Prevalence, risk factors and emergence of extended-spectrum β-lactamase producing-, carbapenem- and colistin-resistant Enterobacterales isolated from wild boar (Sus scrofa) in Tunisia

Selmi, Rachid; Tayh, Ghassan; Srairi, Sinda; Mamlouk, Aymen; Chehida, Faten Ben; Lahmar, Samia; Bouslama, Mongi; Dâaloul-Jedidi, Monia; Messadi, Lilia

doi:10.1016/j.micpath.2021.105385

Cited by 13 publications

(10 citation statements)

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“…mirabilis forms part of the microbiota of the human intestine and has gradually become an important pathogenic bacterium associated with nosocomial infection in recent years. It is naturally resistant to various drugs since it can easily integrate new drug-resistance genes to yield corresponding phenotypes [3, 4]. Due to the widespread use of antimicrobial drugs yielding selective pressure, reports of severe nosocomial infections caused by multiple-resistant bacteria have increased significantly [9, 16–21].…”

Section: Discussionmentioning

confidence: 99%

“…The mechanisms underlying carbapenem resistance in P. mirabilis mainly include reduced outer membrane permeability and carbapenemase production, which are mainly commonly detected in bla KPC , bla NDM , bla VIM , bla IMP and bla OXA , especially bla NDM [3, 4 ]. The bla NDM-1 gene was first described in Klebsiella pneumoniae in a patient returning from India to Sweden in 2008 and has recently been identified in numerous environmental and clinical Gram-negative bacteria [2].…”

Section: Introductionmentioning

confidence: 99%

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Study of the molecular characteristics and homology of carbapenem-resistant Proteus mirabilis by whole genome sequencing

Liu

Wei

et al. 2023

Journal of Medical Microbiology

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Introduction. Proteus mirabilis is part of the family Enterobacteriaceae , and is naturally resistant to various antimicrobial drugs. In recent years, outbreaks of severe nosocomial infections caused by carbapenem-resistant P. mirabilis (CR-PMI) have been frequently reported. Few studies exist on the whole-genome molecular characteristics of this bacterium in China and elsewhere, which stimulated the implementation of this study. Hypothesis. CR-PMI strains contained the multiple drug resistance genes and exhibited a high resistance rate to commonly used antimicrobial drugs. Aim. Our goals here were to identify resistance mechanisms and homology of CR-PMI strains and provide a theoretical basis for clinical treatment and controlling nosocomial infections. Methodology. Bacterial species identification was carried out using matrix-assisted laser desorption/ionization time of flight MS (MALDI-TOF-MS). Antimicrobial susceptibility was determined using the VITEK 2 system and Kirby-Bauer (K-B) disc-diffusion method. Whole-genome sequencing (WGS) was conducted by the Illumina platform NovaSeq sequencer. Antibiotic resistance genes (ARGs) were identified using the NCBI database with Abricate. Plasmid replicon types were identified using PlasmidFinder, available at the Center for Genomic Epidemiology. Results. Five CR-PMI strains collected in our hospital from July 2019 to September 2021 were resistant to almost all antimicrobial agents except aztreonam (ATM), amikacin (AMK) and cefotetan (CTT). All CR-PMI strains contained the carbapenem resistance gene New Delhi metallo-β-lactamase 1 (bla NDM-1), and two strains harboured extended-spectrum β-lactamase (ESBL) genes bla PER-4 and bla CTX-M-65. The five CR-PMI strains contained 27, 18, 30, 25 and 24 drug-resistance genes, respectively. Most antimicrobial resistance genes were detected for aminoglycosides (n=14), followed by cephalosporins (n=7). The phylogenetic tree was divided into five evolutionary groups, and the five CR-PMI strains were in the four evolutionary groups B–E. Conclusion Overall, CR-PMI strains exhibited a high resistance rate to commonly used antimicrobial drugs, and contained the carbapenem resistance gene bla NDM-1. The CR-PMI strains showed a polyclonal trend in different wards at different times. Most importantly, all strains identified contained important antimicrobial resistance genes, which may lead to severe drug resistance transmission and fatal multiple resistant bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study of the molecular characteristics and homology of carbapenem-resistant Proteus mirabilis by whole genome sequencing

Liu

Wei

et al. 2023

Journal of Medical Microbiology

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“…using cephalosporin-additive or the specific-selective agars) to investigate the prevalence of ESC resistance in E. coli isolated from terrestrial wild animals worldwide, including macaque (0%) and wild boars (33.0%) in Algeria [19]; wild boars (4.5%) in Spain [20]; various wild mammals (1.5%) in France [21]; wild boars (1.2%) and ruminants (1.1%) in Germany [22]; wild boars (23.3%) in Italy [23]; badger (8.9%) and fox (11.53%) in Ireland [24]; red foxes (10.5%) and martens (7.5%) in Poland [25]; wild boars (14%) in Tunisia [26]; and raccoons (56.9%) in the United States [9]]. These results implied that the prevalence of ESC-resistant E.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have been conducted in a similar manner [i.e. using cephalosporin-additive or the specific-selective agars) to investigate the prevalence of ESC resistance in E. coli isolated from terrestrial wild animals worldwide, including macaque (0%) and wild boars (33.0%) in Algeria [19]; wild boars (4.5%) in Spain [20]; various wild mammals (1.5%) in France [21]; wild boars (1.2%) and ruminants (1.1%) in Germany [22]; wild boars (23.3%) in Italy [23]; badger (8.9%) and fox (11.53%) in Ireland [24]; red foxes (10.5%) and martens (7.5%) in Poland [25]; wild boars (14%) in Tunisia [26]; and raccoons (56.9%) in the United States [9]]. These results implied that the prevalence of ESC-resistant E. coli isolates from wild animals is greatly different by animal species, which is closely related to their habitat and/or feeding habit and country, and Japanese raccoon dogs are comparable to other terrestrial wild animals in terms of ESC-resistant E. coli reservoir.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in Escherichia coli isolates from Japanese raccoon dogs (Nyctereutes viverrinus) in Kanagawa Prefecture, Japan: Emergence of extended-spectrum cephalosporin-resistant human-related clones

Shimizu

Kido²,

Miyashita

et al. 2022

Journal of Medical Microbiology

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Introduction. Wild animals are one of the putative reservoirs of antimicrobial-resistant bacteria, but the significance of raccoon dogs remains to be investigated. Hypothesis. Raccoon dogs can be a reservoir of antimicrobial-resistant bacteria. Aim. This study aimed to explore the prevalence of antimicrobial resistance, mainly extended-spectrum cephalosporins resistance, in Escherichia coli isolates from faeces of 80 Japanese raccoon dogs in Kanagawa Prefecture, Japan. Methodology. All of the 80 faecal samples were streaked onto deoxycholate-hydrogen sulfate-lactose (DHL) and cefotaxime (CTX)-supplemented DHL (DHL-CTX) agars. Susceptibilities to ten antimicrobials were determined using the agar dilution method. Additionally, extended-spectrum β-lactamases (ESBLs) and AmpC-type β-lactamases (ABLs) were identified in addition to sequence types (STs), in ESC-resistant isolates by a polymerase chain reaction and sequencing. Results. Out of all the samples, 75 (93.8 %) and 20 (25.0 %) E. coli isolates were isolated by DHL and DHL-CTX agars, respectively. Significantly higher resistance rates to most of the drugs were found in DHL-CTX-derived isolates than DHL-derived isolates (P<0.01). Genetic analysis identified CTX-M-14 (n=6), CTX-M-2 (n=2), CTX-M-1 (n=1) and CTX-M-55 (n=1) as ESBLs, and CMY-2 (n=8) and DHA-1 (n=1) as ABLs in 20 DHL-CTX-derived isolates. Most of the detected STs were related to Japanese humans (i.e. ST10, ST58, ST69, ST131, ST357, ST648 and ST4038). Notably, this is the first report on ST69, ST131, ST155 and ST648, which are well-known international high-risk clones in Japanese raccoon dogs. Conclusion. Our findings underscore the need to understand the significance of raccoon dogs as an antimicrobial-resistant bacteria reservoir using one health approach.

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“…In addition, third-generation cephalosporin-resistant E. coli and K. pneumoniae would cause 50,000–100,000 deaths. Many antibiotic-resistance mechanisms have been identified among Enterobacterales and extended-spectrum β-lactamases (ESBLs), which remained one of the most common mechanisms ( Lai et al, 2014 ; Colmenarejo et al, 2020 ; Vink et al, 2020 ; Estaleva et al, 2021 ; Karlowsky et al, 2022 ; Sader et al, 2022 ; Selmi et al, 2022 ). ESBLs that mediate resistance to newer β-lactam antibiotics, including extended-spectrum cephalosporins and monobactams, are plasmid-mediated class A enzymes commonly found in the family Enterobacterales and frequently detected among E. coli and K. pneumoniae .…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of extended-spectrum β-lactamases in Enterobacterales in Taiwan for over two decades

Chao

Lai

2023

Front. Microbiol.

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The emergence of antimicrobial resistance among microorganisms is a serious public health concern, and extended-spectrum β-lactamases (ESBL)-producing Enterobacterales is one of the major concerns among antibiotic-resistant bacteria. Although the prevalence of ESBL in Enterobacterales has been increasing with time, the prevalence of ESBL could differ according to the species, hospital allocation, sources of infections, nosocomial or community acquisitions, and geographic regions. Therefore, we conducted a comprehensive review of the epidemiology of ESBL-producing Enterobacterales in Taiwan. Overall, the rates of ESBL producers are higher in northern regions than in other parts of Taiwan. In addition, the genotypes of ESBL vary according to different Enterobacterales. SHV-type ESBLs (SHV-5 and SHV-12) were the major types of Enterobacter cloacae complex, but Serratia marcescens, Proteus mirabilis, Escherichia coli, and Klebsiella pneumoniae were more likely to possess CTX-M-type ESBLs (CTX-M-3 and CTX-M-14). Moreover, a clonal sequence type of O25b-ST131 has been emerging among urinary or bloodstream E. coli isolates in the community in Taiwan, and this clone was potentially associated with virulence, ESBL (CTX-M-15) production, ciprofloxacin resistance, and mortality. Finally, the evolution of the genetic traits of the ESBL-producing Enterobacterales isolates helps us confirm the interhospital and intrahospital clonal dissemination in several regions of Taiwan. In conclusion, continuous surveillance in the investigation of ESBL production among Enterobacterales is needed to establish its long-term epidemiology.

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Prevalence, risk factors and emergence of extended-spectrum β-lactamase producing-, carbapenem- and colistin-resistant Enterobacterales isolated from wild boar (Sus scrofa) in Tunisia

Cited by 13 publications

References 50 publications

Study of the molecular characteristics and homology of carbapenem-resistant Proteus mirabilis by whole genome sequencing

Study of the molecular characteristics and homology of carbapenem-resistant Proteus mirabilis by whole genome sequencing

Antimicrobial resistance in Escherichia coli isolates from Japanese raccoon dogs (Nyctereutes viverrinus) in Kanagawa Prefecture, Japan: Emergence of extended-spectrum cephalosporin-resistant human-related clones

Epidemiology of extended-spectrum β-lactamases in Enterobacterales in Taiwan for over two decades

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