Prevalence, quantification, and typing of human adenoviruses detected in river water in Taiwan

Huang, Zhon-Min; Hsu, Bing-Mu; Kao, Po-Min; Chang, Tien-Yu; Hsu, Tsui‐Kang; Ho, Ying-Ning; Yang, Yichun; Huang, Yatao

doi:10.1007/s11356-014-4000-7

Cited by 11 publications

(13 citation statements)

References 44 publications

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“…To our knowledge this constitutes the first report of NoV GII.P17 identification in water environments in Mexico. And, although HAdV 41 is reported to be the most prevalent serotype in other locales (Huang et al 2015;Tao et al 2016), the results reported here showed that serotype 40 was most prevalent in the YP karst aquifer, even though serotypes 40 and 41 were detected among the water samples tested.…”

Section: Sadv -18contrasting

confidence: 75%

Norovirus and human adenovirus occurrence and diversity in recreational water in a karst aquifer in the Yucatan Peninsula, Mexico

Rosiles‐González¹,

Ávila‐Torres²,

Moreno‐Valenzuela

et al. 2019

J Appl Microbiol

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Aims To determine the seasonal occurrence and diversity of norovirus (NoV) and human adenovirus (HAdV) in groundwater from sinkholes, and brackish water used for recreational activities in the karst aquifer of the Yucatan Peninsula, Mexico. Methods and results Hollow fibre ultrafiltration was used to concentrate viruses and standard plaque assay methods were used to enumerate somatic and F+ specific coliphages as viral indicators. Real‐time quantitative polymerase chain reaction assays were used to estimate the number of genome copies for NoV strains GI, and GII, and HAdVs. The predominant NoV genotypes and HAdV serotypes were identified by comparative sequence analysis. Somatic and male F+ specific coliphages were detected at concentrations up to 94 and 60 plaque‐forming units per 100 ml respectively. The NoV genogroup I (GI) was associated with 50% of the sampled sites during the rainy season only, at concentrations ranging from 120 to 1600 genome copies per litre (GC l−1). The NoV genogroup II (GII) was detected in 30 and 40% of the sampled sites during the rainy and dry seasons, respectively, at concentrations ranging from 10 to 290 GC l−1. During the rainy and dry seasons, HAdVs were detected in 20% of the sites, at concentrations ranging from 24 to 690 GC l−1. Identification of viral types revealed the presence of NoV GI.2, GII.Pe, GII.P16 and GII.P17, and HAdV F serotypes 40 and 41. Conclusions These findings demonstrate that NoVs and HAdVs are prevalent as virus contaminants in the karst aquifer, representing potential health risks particularly during the rainy season, in one of the most important areas used for tourism in Mexico. Significance and Impact of the Study This is one of the few studies conducted in karst aquifers that provide a foundational baseline of the distribution, concentrations and diversity of NoVs and HadVs in these particular environments.

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Section: Sadv -18contrasting

confidence: 75%

Norovirus and human adenovirus occurrence and diversity in recreational water in a karst aquifer in the Yucatan Peninsula, Mexico

Rosiles‐González¹,

Ávila‐Torres²,

Moreno‐Valenzuela

et al. 2019

J Appl Microbiol

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“…A previous study reported that the average viral loads of HuNoV was 2.30 log 10 genome copies/L in a river in the Netherlands [ 59 ]. Similarly, the viral load of AdenoV ranged from 2.79 to 2.93 log 10 genome copies/L in a river in Taiwan [ 60 ]. Furthermore, RotaV concentrations were as high as 4.14 log 10 genome copies/L in surface river water in Germany [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Weather and Environmental Factors on the Seasonal Prevalence of Foodborne Viruses in Irrigation Waters in Gyeonggi Province, Korea

et al. 2020

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This study aimed to investigate the prevalence of foodborne viruses in reservoirs (an important resource of irrigation water) and its correlation with environmental and weather factors. From May 2017 to November 2018, we visited ten reservoirs and a river in the Anseong region of South Korea and collected a total of 192 samples in accordance with the environment protection agency guidelines. We recorded the weather factors (temperature, humidity, and accumulated precipitation) and investigated the surrounding environment factors (livestock, fishing site, the catchment area of reservoirs, etc.). Our research results show that from the river and reservoirs, the detection rates of human norovirus GII, adenovirus, rotavirus, human norovirus GI, and astrovirus were 27.1, 10.4, 10.4, 4.16, and 3.1%, respectively. Their viral load ranged from −1.48 to 1.55 log10 genome copies/l. However, hepatitis A virus was not detected in any irrigation water sample. Although no sampling was performed in winter, foodborne viruses and male-specific coliphages were frequently found during spring (40.78%) and autumn (39.47%). Interestingly, the significant correlation between the accumulative precipitation and the number of detected norovirus and adenovirus was confirmed by linear regression analysis. Furthermore, when the accumulative precipitation ranged from 20 to 60 mm, it significantly affected the viral load and prevalence. Among the environmental factors, recreational facilities such as fishing sites and bungalow fishing spots were identified as contamination sources by correlation analysis. Our research results confirmed the correlations between environmental contamination factors in the reservoir and weather factors with the prevalence of foodborne viruses in the reservoir. These facilitates the assessment of potential foodborne virus contamination during crop irrigation. In addition, predictive models including environmental and weather factors should be developed for monitoring and controlling the safety of irrigation waters in reservoirs.

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“…To enhance virus recovery from the samples, the water samples were concentrated before analysis. Water samples (1 L) were vacuum‐filtered through a 42 mm GN‐6 membrane with a pore size of 0.45 μm (GN‐Metricel PALL) (Huang et al., 2015 ). Next, the filter paper was placed in 45 mL of phosphate‐buffered saline (PBS, pH 7.2) and kneaded for 5 min with sterile gloves.…”

Section: Methodsmentioning

confidence: 99%

Prevalence, Distribution, and Genotypes of Adenovirus and Norovirus in the Puzi River and Its Tributaries and the Surrounding Areas in Taiwan

et al. 2021

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This study investigated the prevalence, distribution, and genotypes of adenoviruses (AdVs) and noroviruses (NoVs) in the Puzi River and surrounding areas in Taiwan. The viruses in the water samples were isolated using the membrane filtration method and the viral nucleic acids were extracted. The RNA of NoVs was reverse‐transcribed into complementary DNA using reverse transcriptase‐polymerase chain reaction. AdVs and NoVs were detected using nested PCR. Genotyping and phylogenetic analyses were performed to identify the various viral genotypes in the water samples. Human adenovirus (HAdVs) and porcine adenovirus (PAdVs) were the predominant genotypes in the water samples. The prevalence of F species HAdVs serotype 41 (79.2%) and C species PAdVs serotype 5 (18.1%) was higher than that of other serotypes. Among NoVs, genogroup GII was more prevalent than GI. In particular, GII.4 (21.2%) and GII.17 (18.2%) were the predominant genotypes, which was consistent with the clinical findings. The prevalence of both AdVs and NoVs was higher in the winter than spring, summer and autumn seasons. AdVs and NoVs detection results were statistically analyzed by investigating their association with water quality indicators. The results revealed that the presence of AdVs was significantly correlated with the heterotrophic bacterial count, total coliform Escherichia coli , turbidity, salinity, and dissolved oxygen. Meanwhile, the presence of NoVs was only significantly correlated with temperature, pH, and dissolved oxygen. Microbial pollution sources may include urban runoff and discharge of water from livestock farms situated near the river and tributaries within this region of Taiwan. Future studies should include comparisons of the presence of AdVs and NoVs in these known pollution sources and water quality monitoring of these watersheds, as this will allow potential identification of pollution sources. Additionally, remediation strategies must be developed to minimize viral contamination in the river ecosystem.

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Prevalence, quantification, and typing of human adenoviruses detected in river water in Taiwan

Cited by 11 publications

References 44 publications

Norovirus and human adenovirus occurrence and diversity in recreational water in a karst aquifer in the Yucatan Peninsula, Mexico

Norovirus and human adenovirus occurrence and diversity in recreational water in a karst aquifer in the Yucatan Peninsula, Mexico

Effects of Weather and Environmental Factors on the Seasonal Prevalence of Foodborne Viruses in Irrigation Waters in Gyeonggi Province, Korea

Prevalence, Distribution, and Genotypes of Adenovirus and Norovirus in the Puzi River and Its Tributaries and the Surrounding Areas in Taiwan

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