Prevalence, populations and pheno- and genotypic characteristics of Listeria monocytogenes isolated from ready-to-eat vegetables marketed in São Paulo, Brazil

Sant’Ana, Anderson S.; Igarashi, Maria Crystina; Landgraf, Mariza; Destro, Maria Teresa; Franco, Bernadette D.G.M.

doi:10.1016/j.ijfoodmicro.2011.12.036

Cited by 102 publications

(66 citation statements)

References 73 publications

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“…Similar results were observed in a posterior study with human clinical specimens [18]. Recent studies also described the high occurence of 4b serotype in dairy products [17,30], ready-to-eat vegetables [31] and meat products retailed [6] in Brazil. Although the wide distribution of serotype 4b has been described in Brazil, details about the occurrence of ECs and your association with sporadic cases or outbreaks is ignored.…”

Section: Discussionsupporting

confidence: 86%

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Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Barbosa

Cerqueira

Rusak

et al. 2015

J Infect Dev Ctries

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Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECIIpositive; and two ECI strains from a human case (1982) and from bovine meat (2009). Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.

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Section: Discussionsupporting

confidence: 86%

“…A previous study evaluated the virulence potential of 4b and 1/2b serotypes isolated from vegetable salad samples in São Paulo (Brazil), by searching for the inlA, inlC and inlJ genes, finding similar results [31].…”

Section: Discussionmentioning

confidence: 94%

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Barbosa

Cerqueira

Rusak

et al. 2015

J Infect Dev Ctries

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“…18 In addition, in a research report on storage temperatures, L. monocytogenes growth in fresh produce at 7 °C and 15 °C for 6 days was compared. 19 The result showed that the population of L. monocyogenes increased more at 15 °C over the storage period. Similar findings have been reported on the effect of storage conditions on L. monocytogenes growth at 3 different temperatures (4 °C, 12 °C and 22 °C) for comparison.…”

Section: Discussionmentioning

confidence: 94%

Detection of Listeria Species in Fresh Produce Samples from Different Retail Shops in Canterbury, New Zealand

Zhu¹,

Gooneratne²,

Hussain³

2016

Adv Food Technol Nutr Sci Open J

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“…The higher global resistance of E. coli O157:H7 to sanitizers widely used by the fresh-produce industry, such as SH and CD, can provide further insights on the reasons why this pathogen is commonly involved in fresh-produce disease outbreaks (68). Despite the fact that L. monocytogenes presented similar resistances to almost all the sanitizers deemed also ineffective against E. coli O157:H7 (Table 2), it is known that the former pathogen is more susceptible to inhibition in vegetables (69,70). Salmonella was found to be more resistant than E. coli O157:H7 and L. monocytogenes only when water was assessed as a washing treatment (Table 2).…”

Section: Figmentioning

confidence: 99%

Meta-analysis of the Effects of Sanitizing Treatments on Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes Inactivation in Fresh Produce

Prado-Silva

Cadavez

Gonzales-Barrón

et al. 2015

Appl Environ Microbiol

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The aim of this study was to perform a meta-analysis of the effects of sanitizing treatments of fresh produce on Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. From 55 primary studies found to report on such effects, 40 were selected based on specific criteria, leading to more than 1,000 data on mean log reductions of these three bacterial pathogens impairing the safety of fresh produce. Data were partitioned to build three meta-analytical models that could allow the assessment of differences in mean log reductions among pathogens, fresh produce, and sanitizers. Moderating variables assessed in the meta-analytical models included type of fresh produce, type of sanitizer, concentration, and treatment time and temperature. Further, a proposal was done to classify the sanitizers according to bactericidal efficacy by means of a meta-analytical dendrogram. The results indicated that both time and temperature significantly affected the mean log reductions of the sanitizing treatment (P < 0.0001). In general, sanitizer treatments led to lower mean log reductions when applied to leafy greens (for example, 0.68 log reductions [0.00 to 1.37] achieved in lettuce) compared to other, nonleafy vegetables (for example, 3.04 mean log reductions [2.32 to 3.76] obtained for carrots). Among the pathogens, E. coli O157:H7 was more resistant to ozone (1.6 mean log reductions), while L. monocytogenes and Salmonella presented high resistance to organic acids, such as citric acid, acetic acid, and lactic acid (ϳ3.0 mean log reductions). With regard to the sanitizers, it has been found that slightly acidic electrolyzed water, acidified sodium chlorite, and the gaseous chlorine dioxide clustered together, indicating that they possessed the strongest bactericidal effect. The results reported seem to be an important achievement for advancing the global understanding of the effectiveness of sanitizers for microbial safety of fresh produce.T he consumption of fresh fruits and vegetables comprises an essential element of a healthy diet and a protective factor against several chronic diseases (1, 2). Even though the ingestion of these products is highly recommended by health authorities, guaranteeing fresh, safe, and high-quality fruits and vegetables remains an enormous challenge for fresh-produce industries.In order to deliver the health benefits (2), fruits and vegetables must be safe. One of the chief concerns related to the safety of these products is their recurrent and increased association with disease outbreaks (3-6). Epidemiological investigations indicate that Salmonella, pathogenic Escherichia coli, and Listeria monocytogenes stand out as the most important bacterial agents linked to fresh-produce disease outbreaks (3, 5-7). Recent studies have reported the occurrence and high diversity of these microorganisms in the environment or in close areas of produce farming areas (8-13).Given the above, fresh-produce industries have been implementing measures at pre-and postharvest steps to reduce or avoid the contam...

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Prevalence, populations and pheno- and genotypic characteristics of Listeria monocytogenes isolated from ready-to-eat vegetables marketed in São Paulo, Brazil

Cited by 102 publications

References 73 publications

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Detection of Listeria Species in Fresh Produce Samples from Different Retail Shops in Canterbury, New Zealand

Meta-analysis of the Effects of Sanitizing Treatments on Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes Inactivation in Fresh Produce

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