Prevalence, Phylogroups and Antimicrobial Susceptibility of Escherichia coli Isolates from Food Products

Abstract

The emergence of multi-drug resistant E. coli is an important matter of increasing considerable concern to global public health. The aim of this study was to investigate the incidence, antibiotic resistance pattern and phylogroups of E. coli isolates obtained from raw milk, vegetable salad and ground meat samples collected from Qazvin Province (Iran). Culture-based techniques, Kirby-Bauer disk diffusion susceptibility testing and PCR assays were used to determine the incidence rate, antimicrobial resistance pattern and phylogenetic groups of the E. coli isolates. The E. coli isolates were highly resistant to amoxicillin (79.1%), trimethoprim-sulfamethoxazole (70.8%), amoxicillin-clavulanic acid (62.5%), tetracycline (54.1%), chloramphenicol (54.1%), nitrofurantoin (54.1%), ampicillin (45.8%), streptomycin (45.8%), and kanamycin (33.3%); and completely susceptible to norfloxacin and azithromycin and 70.8% of the isolates were multi-drug resistant. Most E. coli isolates (46%) belonged to phylogroup A. Novel, practical, efficient food safety control and surveillance systems of multi-drug resistant foodborne pathogens are required to control the foodborne pathogen contamination.

Prevalence of virulence genes among Escherichia coli strains isolated from food and carcass swabs of different animal origins in Croatia

“…In the 100 E.coli strains analysed in this study, most were assigned to the A and B1 phylogenetic groups, including virulence gene-positive strains. These data are in agreement with the prevalence of the A and B1 groups in commensal strains isolated from different food and animal species (4,9,38) and the occurrence of enteric E. coli within these groups (8). The results of the study also indicate the phylogenetic group tendency toward a specific host (P = 0.039), with group A more frequently distributed in strains of pig origin and B1 more prevalent in game and cattle strains.…”

Multiple-Drug Resistant Shiga Toxin-Producing E. coli in Raw Milk of Dairy Bovine

“…Antimicrobial-resistant bacteria are one of the most serious public health issues and are predicted to cause the death of millions of people annually beginning in 2050 [ 33 ]. MDR strains have also been reported in China and Iran [ 34 ]. The prevalence of ESBL genes, with bla CTX-M being the most predominant, emphasizes the importance of monitoring and addressing antibiotic resistance in STEC strains to address significant challenges to food safety [ 35 ].…”

Evaluation of Polyphenolic Composition and Antimicrobial Properties of Sanguisorba officinalis L. and Sanguisorba minor Scop.

“…A variation of the Kirby-Bauer diffusion method, a simple and rapid technique that determines the spectrum of sensitivity/resistance to antibiotics of microorganisms, was used for quantificative evaluation of antimicrobial activity, a variant in which the free discs were loaded with 50 µL of the extracts [79]. The test was applied for all extracts obtained from plant organs of S. officinalis L. and S. minor Scop.…”

“…Using a Drigalski rod, 500 µL of the 1/10 dilution of the 0.5 McFarland suspension was dispersed on top of the agarized Muller-Hinton medium. As soon as the plates were sowered, they were left for 3-5 min to absorb the inoculum before applying SOR, SOL, SOF, SMR, SML, and SMF extracts on a 50 µL space on disk [79]. After the spots were placed and the volume was placed in each well, the plates were thermostated at 35 ± 2 • C, under aerobic conditions, for 16-18 h.…”