Prevalence, phylogeny, and antimicrobial resistance of Escherichia coli pathotypes isolated from children less than 5 years old with community acquired- diarrhea in Upper Egypt

Khairy, Rasha; Fathy, Zahra Atef; Mahrous, Doaa; Mohamed, Ebtisam S.; Abdelrahim, Soha S.

doi:10.1186/s12879-020-05664-6

Cited by 29 publications

(31 citation statements)

References 41 publications

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“…These results are higher than reports of previous studies in Egypt, with ESBL prevalence range from 52–69.6%. 16 , 22 , 34 , 35 In the same context as our results, Valverdi et al 36 documented that acute diarrhoea is a frequent reason for intestinal colonization by ESBL producers that reached to 55.5% in acute diarrhoea. This can be explained by the change in the gut flora that occurs in acute diarrhoea, causing predominance of pathogenic bacterial strains.…”

Section: Discussionsupporting

confidence: 84%

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Comparative Study of CTX-M-15 Producing Escherichia coli ST131 Clone Isolated from Urinary Tract Infections and Acute Diarrhoea

Abdelrahim

Fouad

Abdallah

et al. 2021

IDR

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Background and Purpose The alarming increase in the prevalence of CTX-M-15 extended-spectrum β-lactamase (ESBL) producing E. coli has been significantly linked to the clonal expansion of emerging sequence type (ST131). This study aimed to screen for the O16/O25-ST131 clones among different phylogenetic types of E. coli strains isolated from urinary and diarrhoeal samples. Methods A total of 205 E. coli strains isolated from patients with UTI and acute diarrhoea were investigated by phenotypic and genotypic methods for ESBL identification. Molecular methods were used for identification of O25/O16-ST131 clone and phylogenetic typing of E. coli isolates. Results O25-ST131 clone was detected in 89/105 (84.8%) and 47/100 (47%) of urinary and intestinal E. coli isolates, respectively, with a significant difference ( P -value<0.001). There was a significant high rate of occurrence of ESBLs, MDR, and antibiotic resistance to most antibiotic classes among O25-ST131 than non-O25-ST131 isolates. CTX-M-15 gene was detected in 64/71 (90%) of ESBLs producing intestinal isolates and 54/79 (68.4%) of urinary ESBLs producing isolates. The O25-ST131 clone was reported among all phylogenetic groups. The O16-ST131 clone serotype was not detected in the study isolates. Conclusion High prevalence of the O25-ST131 clone was reported among extraintestinal and intestinal E. coli isolates. First detection of the O25-ST131 clone among phylogenetic groups other than group B2 draws attention of the ability of this clone to transfer among commensal groups. An increasing in the prevalence of CTX-M-15 among E. coli strains especially of intestinal origin is alarming as the intestine is the main reservoir for ExPEC strains causing UTI.

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Section: Discussionsupporting

confidence: 84%

“… 15 E. coli isolate from a previous study was used as quality control in antimicrobial susceptibility testing. 16 …”

Section: Methodsmentioning

confidence: 99%

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Comparative Study of CTX-M-15 Producing Escherichia coli ST131 Clone Isolated from Urinary Tract Infections and Acute Diarrhoea

Abdelrahim

Fouad

Abdallah

et al. 2021

IDR

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“…A Brazilian study reported higher rates of antibiotic resistance among typical EPEC strains than among the atypical ones [90]. A major concern nowadays is the emergence of multidrug-resistant strains, which are frequently reported around the globe [11,90,91].…”

Section: Clinical Aspectsmentioning

confidence: 99%

Enteropathogenic Escherichia coli—A Summary of the Literature

Mare

Ciurea

Man

et al. 2021

Gastroenterology Insights

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Diarrheal disease is still a major public health concern, as it is still considered an important cause of death in children under five years of age. A few decades ago, the detection of enteropathogenic E. coli was made by detecting the O, H, and K antigens, mostly by agglutination. The recent protocols recommend the molecular methods for diagnosing EPEC, as they can distinguish between typical and atypical EPEC by identifying the presence/absence of specific virulence factors. EPEC are defined as diarrheagenic strains of E. coli that can produce attaching and effacing lesions on the intestinal epithelium while being incapable of producing Shiga toxins and heat-labile or heat-stable enterotoxins. The ability of these strains to produce attaching and effacing lesions enable them to cause localized lesions by attaching tightly to the surface of the intestinal epithelial cells, disrupting the surfaces of the cells, thus leading to the effacement of the microvilli. EPEC are classified on typical and atypical isolates, based on the presence or absence of E. coli adherence factor plasmids. All the EPEC strains are eae positive; typical EPEC strains are eae+, bfpA+, while atypical strains are eae+, bfpA−. No vaccines are currently available to prevent EPEC infections.

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“…Interestingly, ESBL production was determined phenotypically in approximately thirty-seven percent of our isolates. In a similar study, 60.6% frequency of ESBL positive strains have been stated byKhairy et al (2020) in Egypt[16]. In view of the genes encoding ESBLs,Khoshvaght et al (2014) reported a high prevalence of bla TEM and bla CTX−M genes in EAEC isolates (78.9% and 63.1%, respectively)[43] Ali et al (2014).…”

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confidence: 85%

The Emergence of Extended-Spectrum β-Lactamase Producing and Hybrid Pathotypes of Escherichia Coli Isolates from Diarrheic Human Cases: A New Public Health Concern in Iran

Asadi¹,

Ghanbarpour²,

Kalantar-Neyestanaki³

et al. 2021

Preprint

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Background: Intra-intestinal pathogenic strains of Escherichia coli are responsible for mild to severe gastrointestinal lesions, which are mediated by various virulence factors. In this study, we have focused on a comprehensive set of E. coli pathotypes, including STEC, EPEC, EHEC, ETEC, and some hybrid pathotypes including, STEC/ ETEC, EHEC/ ETEC, and EPEC/ ETEC.Methods: Totally, 467 stool samples were obtained from gastrointestinal patients during four years (2016 to 2020). Four pathotypes of E. coli (EPEC, ETEC, EHEC, STEC) were screened due to six virulence genes, including eae, stx1, stx2, st, and lt using the conventional PCR method. Finally, detected E. coli pathotypes were subjected to determine phenotypic and genotypic β-lactam resistance properties.Result: In this study 59/467 (12.63%) strains belonged four pathotypes including STEC (20/59; 50.8%), EPEC (11/59; 18.6%), ETEC (8/59 13.5%), EHEC (5/59; 8.4%) and three novel hybrid pathotypes including STEC/ETEC (3/59; 5%), EHEC/ETEC (1/59; 1.6%), and EPEC/ETEC (1/59; 1.6%). Totally 23/59 (38.9%) isolates were identified as ESBL-producing. Conclusion: Transmission of virulence and antibiotic resistance genes among E. coli strains lead to the emergence of antimicrobial-resistant hybrid pathogenic strains, which is an important health concern. According to food-born and fecal-oral transmission of these E. coli strains, standard methods should be performed to eliminate the possible contamination of food, equipment, and living environment to manure and feces.

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Prevalence, phylogeny, and antimicrobial resistance of Escherichia coli pathotypes isolated from children less than 5 years old with community acquired- diarrhea in Upper Egypt

Cited by 29 publications

References 41 publications

Comparative Study of CTX-M-15 Producing Escherichia coli ST131 Clone Isolated from Urinary Tract Infections and Acute Diarrhoea

Comparative Study of CTX-M-15 Producing Escherichia coli ST131 Clone Isolated from Urinary Tract Infections and Acute Diarrhoea

Enteropathogenic Escherichia coli—A Summary of the Literature

The Emergence of Extended-Spectrum β-Lactamase Producing and Hybrid Pathotypes of Escherichia Coli Isolates from Diarrheic Human Cases: A New Public Health Concern in Iran

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