Prevalence of Yersinia enterocolitica in milk and dairy products and the effects of storage temperatures on survival and virulence gene expression

Ahmed, Heba A.; Tahoun, Asmaa B.M.B.; Elez, Rasha M.M. Abou; El-Hamid, Marwa I. Abd; Ellatif, Samah S. Abd

doi:10.1016/j.idairyj.2019.02.010

Cited by 28 publications

(19 citation statements)

References 51 publications

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“…enterocolitica in raw bulk-tank milk from different species (P = 23%) agrees with results reported by Ahmed et al . [ 13 ] (P = 22%) and Hadef et al . [ 67 ] (P = 17%) while it differs from other authors’ reports.…”

Section: Discussionmentioning

confidence: 94%

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Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

et al. 2022

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Yersiniosis is the third most reported food-borne zoonosis in Europe. The aim of the present study was to perform the search for Yersinia enterocolitica in food samples collected from Apulia and Basilicata regions (Southern Italy) and to characterize any isolates by classical and modern analytical methods. A total of 130 samples were analyzed between July 2018 and July 2019: most of them were raw milk and dairy products made from it. Furthermore, 8 out of 130 samples were individual milk samples collected from bovines reared in a Brucella-free farm which showed false positive serological reaction for brucellosis due to the presence of pathogenic Y. enterocolitica O:9 biotype 2 in faeces. The Real Time PCR targeting the ail gene and the culture method were performed to detect pathogenic Y. enterocolitica. Isolates were subjected to API 20E (Biomerieux) and MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization Time-of-Flight) for species identification. All samples were negative for the ail gene. The culture method allowed to isolate suspicious colonies from 28 samples. The API 20E system and the MALDI-TOF MS technique identified 20 Y. enterocolitica and 1 Y. intermedia in a concordant way. The remaining 7 strains were all identified as Y. enterocolitica by the API 20E system, while the MALDI-TOF MS recognized 4 Y. intermedia, 1 Y. bercovieri and 2 Y. massiliensis. Genotypic characterization of the discordant strains was performed by rMLST and it confirmed the MALDI-TOF MS’ results. Only non-pathogenic Y. enterocolitica biotype 1A strains were found, although with a non-negligible prevalence (P = 0.15 with CI 95% = ± 0.06). This study indicates a poor circulation of pathogenic Y. enterocolitica in food products made and marketed in the investigated areas. However, the small number of samples, insufficient for some food categories such as meat and vegetable, does not allow to exclude the presence of pathogenic strains at all.

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Section: Discussionmentioning

confidence: 94%

“…On the contrary, a lot of studies report the presence of pathogenic Y . enterocolitica in samples of raw milk collected from various animal species, as consequence of faecal contamination during milking [ 13 , 14 , 39 – 43 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

et al. 2022

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“…Biotyping of Y. enterocolitica isolates was determined according to the scheme of Wauters (1981) as shown in Table (1).…”

Section: Biotyping Of Y Enterocolitica Isolatesmentioning

confidence: 99%

Phenotypic and genotypic studies on antibiotic resistant Yersinia enterocolitica isolated from milk and milk products in Kaliobia, Egypt

Abdel‐Wahab

El-Tawab

Abdallah

et al. 2021

Benha Veterinary Medical Journal

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The study was applied on 175 random samples of milk and milk products (white cheese, kareish cheese, yoghurt and ice cream) collected from different shops (35 of each), at Kaliobia governorate, Egypt, for detection of Y. enterocolitica strains, beside the phenotypic characterization and detection of some antibiotic resistant virulence genes in the examined samples. The results revealed that 9(10.9%) Y. enterocolitica isolates, bio types 1A and 1B only were isolated from milk and Kareish cheese samples (5/14.3% for each), (4/11.4%) white cheese, (3/8.6%) ice cream, and (2/5.7%) from yoghurt samples. The antibiotic sensitivity profile showed that, the isolated Y. enterocolitica isolates were very high resistant for Penicillin-G followed by methicillin, ampicillin, oxytetracycline, amoxicillin, ampicillin, streptomycin and erythromycin. Meanwhile, they were highly sensitive to meropenem and norfloxacin followed by gentamycin, ciprofloxacin, cefotaxime and fluorophenol. PCR declared that blaTEM and tetA genes were detected in all eight studied Y. enterocolitica isolates. So, it was concluded that, the presence of antimicrobial resistant Y. enterocolitica strains in dairy products could be a public health concern for the consumers.

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“…The molecular methods are more specific, accurate for the identification of Y. enterocolitica than that based on biochemical and morphological characteristics. Five isolates were confirmed as Y. enterocolitica using gene targeting 16 srRNA which is used in a wide range for specificity of Yersinia species as reported by [29,48,49].…”

Section: Disscusionmentioning

confidence: 97%

Incidence of Yersinia enterocolitica in raw farm and market milk sample

EL-Toukhy

El-Sayed

Shoeib

2021

Mansoura Veterinary Medical Journal

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Prevalence of Yersinia enterocolitica in milk and dairy products and the effects of storage temperatures on survival and virulence gene expression

Cited by 28 publications

References 51 publications

Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

Phenotypic and genotypic studies on antibiotic resistant Yersinia enterocolitica isolated from milk and milk products in Kaliobia, Egypt

Incidence of Yersinia enterocolitica in raw farm and market milk sample

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