Prevalence of WU and KI polyomaviruses in plasma, urine, and respiratory samples from renal transplant patients

Csoma, Eszter; Mészáros, Beáta; Asztalos, László; Kónya, József; Gergely, Lajos

doi:10.1002/jmv.22083

Cited by 19 publications

(25 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the exception of JCPyV we did not find any known or unknown HPyVs in the samples using the RCA method. These results are consistent with data from others, who either could not detect HPyV-6, -7, -9, KIPyV, WUPyV, TSPyV or MWPyV in urine samples using real-time PCR [44,45] or reported detection only rarely and at very low concentrations (HPyV-9, KIPyV and WUPyV) [3,9,46,47]. …”

Section: Discussionsupporting

confidence: 81%

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

Kluba

Linnenweber-Held²,

Heim³

et al. 2015

Intervirology

View full text Add to dashboard Cite

Objectives: Multiple novel human polyomaviruses (HPyVs) have been discovered in the last few years. These or other, unknown, nephrotropic HPyVs may potentially be shed in urine. Methods: To search for known and unknown HPyVs we investigated BKPyV-negative urine samples from 105 renal transplant recipients (RTR) by rolling circle amplification (RCA) analysis and quantitative JCPyV PCR. Clinical data was analysed to identify risk factors for urinary polyomavirus shedding. Results: In 10% (11/105) of the urine samples RCA with subsequent sequencing revealed JCPyV, but no other HPyV sequences. Using quantitative JCPyV PCR, 24% (25/105) of the samples tested positive. Overall sensitivities of RCA of 44% (11/25) in detecting JCPyV in JCPyV DNA-positive urine and 67% (10/15) for samples with JCPyV loads >10,000 copies/ml can be assumed. Despite frequent detectable urinary shedding of JCPyV in our cohort, this could not be correlated with clinical risk factors. Conclusion: Routine urinary JCPyV monitoring in BKPyV-negative RTR without suspected polyomavirus-associated nephropathy might be of limited diagnostic value. As RCA works in a sequence-independent manner, detection of novel and known polyomaviruses shed in sufficient quantities is feasible. High-level shedding of HPyVs other than BKPyV or JCPyV in the urine of RTR is unlikely to occur.

show abstract

Section: Discussionsupporting

confidence: 81%

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

Kluba

Linnenweber-Held²,

Heim³

et al. 2015

Intervirology

View full text Add to dashboard Cite

show abstract

“…In this study, we could not isolate WU, KI, MC and TS from any urine samples of pediat- ric patients with SLE. By contrast, WU and KI viruria were recently reported in kidney transplant recipients (Csoma et al 2011). Conditions specific to the transplant process such as ischemic kidney allograft injury may increase susceptibility of transplant recipients to WU and KI reactivation.…”

Section: Discussionmentioning

confidence: 92%

Polyomavirus Reactivation in Pediatric Patients with Systemic Lupus Erythematosus

Rianthavorn

Posuwan

Payungporn

et al. 2012

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Polyomavirus (PyV) infection usually persists without any symptoms in normal individuals. In immunocompromised patients including patients with systemic lupus erythematosus (SLE), PyV reactivation occurs with a high prevalence and can cause severe clinical complications. In this study, reactivation of six PyV [JC, BK, WU, KI, merkel cell (MC) and trichodysplasia spinulosa (TS)] was investigated in terms of prevalence, clinical implications and correlation with urine transforming growth factor (TGF)-β1 expression in 50 SLE patients aged less than 18 years. Clinical characteristics were obtained from medical record review. PyV viruria was assessed by nested polymerase chain reaction. Urine TGF-β1 was measured with ELISA. The mean age was 13 ± 2.8 years. The prevalence of JC and BK viruria was 16% and 32%, respectively. WU, KI, MC and TS were not isolated from any urine specimens. Co-reactivation of 2 PyV was not detected. Urine TGF-β1 levels in patients with JC viruria, with BK viruria and without PyV viruria were 0.27 ± 0.09, 0.10 ± 0.05 and 0.13 ± 0.09 ng/mg of urine creatinine, respectively. Cumulative doses of cyclophosphamide per body weight and urine TGF-β1 levels were higher in JC viruria than in other groups (p < 0.05). The prevalence of JC and BK reactivation was higher in pediatric patients with SLE than in the normal population. JC reactivation in pediatric patients with SLE was correlated with the administration of high-dose cyclophosphamide and increased urine TGF-β1 levels. Surveillance of JC reactivation is recommended in pediatric patients with chronic and severe SLE receiving high-dose cyclophosphamide.

show abstract

“…To detect WU and KI virus DNA, nested and duplex real-time PCRs were carried out with the primers [58,59] and protocols detailed previously [39,40]. Plasmids containing the KIPyV and WUPyV genomes were used as positive controls kindly provided by Tobias Allander from Karolinska Institute (Stockholm, Sweden) and David Wang from Washington University (St. Louis, Miss., USA) [21,60].…”

Section: Methodsmentioning

confidence: 99%

“…Their respiratory transmission is suggested based on prevalence data from numerous studies, as has been summarized in review articles [19,37,38]. Both KI and WU viruses were detected in urine samples, which suggests that these viruses may also infect the urinary tract [39,40,41]. …”

Section: Introductionmentioning

confidence: 99%

No Evidence of Human Polyomavirus 9, WU and KI DNA in Kidney and Urinary Bladder Tumour Tissue Samples

Csoma¹,

Bidiga

Méhes

et al. 2016

Pathobiology

Self Cite

View full text Add to dashboard Cite

Background/Aims: The oncogenic potential of human polyomaviruses (HPyVs) has been proposed, but so far only Merkel cell carcinoma polyomavirus seems to be associated with a human tumour. The role of BK polyomavirus (BKPyV) in human tumourigenesis remains controversial. BKPyV establishes persistent infection in the urinary tract, and renal and bladder neoplasms have been studied extensively, but conflicting prevalence data are reported. KI, WU and HPyV9 were detected in urine samples suggesting that these viruses may also infect the urinary tract, but their presence in urinary tract tumours has not been studied. The aim of this work was to examine the prevalence of KIPyV, WUPyV, HPyV9 and BKPyV by PCR in renal and bladder neoplasms. Methods: A total of 190 formalin-fixed paraffin-embedded renal neoplasms, bladder cancer and kidney biopsy samples were analysed for the presence of BKPyV, KIPyV, WUPyV and HPyV9 DNA by real-time and nested PCR. Results: Amplifiable DNA was extracted from all the samples, but none of the studied viruses were detected in benign renal neoplasia (0/23), malignant renal tumours (0/89) or bladder cancer (0/76). Conclusion: Our study did not find any evidence that BKPyV, KIPyV, WUPyV or HPyV9 are associated with bladder and renal tumours.

show abstract

Prevalence of WU and KI polyomaviruses in plasma, urine, and respiratory samples from renal transplant patients

Cited by 19 publications

References 17 publications

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding

Polyomavirus Reactivation in Pediatric Patients with Systemic Lupus Erythematosus

No Evidence of Human Polyomavirus 9, WU and KI DNA in Kidney and Urinary Bladder Tumour Tissue Samples

Contact Info

Product

Resources

About