Prevalence of Virulence Genes of Diarrheagenic &lt;i&gt;Escherichia coli&lt;/i&gt; in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh

Parvej, Md. Shafiullah; Alam, Ashraful; Shono, Mio; Zahan, Mst. Nusrat; Parvez, Misrat Masuma; Ansari, Wahedul Karim; Jowel, Sayduzzaman; Uddi̇n, Mai̇n; Kage-Nakadai, Eriko; Rahman, Md. Tanvir; Nishikawa, Yoshikazu

doi:10.7883/yoken.jjid.2019.016

Cited by 6 publications

(4 citation statements)

References 31 publications

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“…Studies from many countries have detected drug resistance determinants in APEC [ 27 , 28 ]. In Bangladesh, previous studies have identified antibiotic-resistant E. coli from poultry [ 29 , 30 , 31 , 32 ]. These studies, however, did not focus on APEC and the associated virulence genes.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

et al. 2020

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Avian pathogenic Escherichia coli (APEC) causes significant economic losses in poultry industries. Here, we determined for the first time in Bangladesh, the prevalence of APEC-associated virulence genes in E. coli isolated from layer farms and their antibiotic resistance patterns. A total of 99 samples comprising internal organs, feces, and air were collected from 32 layer farms. Isolation was performed by culturing samples on eosin–methylene blue agar plates, while the molecular detection of APEC was performed by PCR, and antibiograms were performed by disk diffusion. Among the samples, 36 were positive for the APEC-associated virulence genes fimC, iucD, and papC. Out of 36 isolates, 7, 18, and 11 were positive, respectively, for three virulence genes (papC, fimC, and iucD), two virulence genes, and a single virulence gene. Although the detection of virulence genes was significantly higher in the internal organs, the air and feces were also positive. The antibiograms revealed that all the isolates (100%) were resistant to ampicillin and tetracycline; 97.2%, to chloramphenicol and erythromycin; 55.5%, to enrofloxacin; 50.0%, to norfloxacin and ciprofloxacin; 19.4%, to streptomycin; 11.1%, to colistin; and 8.33%, to gentamicin. Interestingly, all the isolates were multidrug-resistant (MDR). Spearman’s rank correlation coefficient analysis revealed the strongest significant correlation between norfloxacin and ciprofloxacin resistance. This is the first study in Bangladesh describing the molecular detection of APEC in layer farms. Isolated APEC can now be used for detailed genetic characterization and assessing the impact on public health.

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Section: Introductionmentioning

confidence: 99%

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

et al. 2020

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“…It could due to the complex pathogenesis of E. coli, whose ability to exert virulence on the host depends on the balance between the state of the host and the presence and expression of E. coli virulence factors. Therefore, the in vitro results of virulence factors cannot reflect the in vivo conditions and the complex immune reactions after E. coli infection [35][36][37]. In addition, although O serotyping is important to determine the virulence of E. coli [38,39], the method has its own limitations: antisera produced against specific O groups may cross-react, and another being that some E. coli may lack O antigens and cannot be isolated or identified [40].…”

Section: Discussionmentioning

confidence: 99%

Immunological pathogenesis of Bovine E. coli infection in a model of C. elegans

et al. 2022

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Background Cattle industry is critical for China’s livestock industry, whereas E. coli infection and relevant diseases could lead huge economic loss. Traditional mammalian models would be costly, time consuming and complicated to study pathological changes of bovine E. coli. There is an urgent need for a simple but efficient animal model to quantitatively evaluate the pathological changes of bovine-derived E. coli in vivo. Caenorhabditis elegans (C. elegans) has a broad host range of diverse E. coli strains with advantages, including a short life cycle, a simple structure, a transparent body which is easily visualized, a well-studied genetic map, an intrinsic immune system which is conservable with more complicated mammalians. Results Here, we considered that O126 was the dominant serotype, and a total of 19 virulence factors were identified from 41 common E. coli virulence factors. Different E. coli strains with diverse pathogenicity strengths were tested in C. elegans in E. coli with higher pathogenicity (EC3/10), Nsy-1, Sek-1 and Pmk-1 of the p38 MAPK signaling pathway cascade and the expression of the antimicrobial peptides Abf-3 and Clec-60 were significantly up-regulated comparing with other groups. E. coli with lower pathogenicity (EC5/13) only activated the expression of Nsy-1 and Sek-1 genes in the p38 MAPK signaling pathway, Additionally, both groups of E. coli strains caused significant upregulation of the antimicrobial peptide Spp-1. Conclusion Thirteen E. coli strains showed diverse pathogenicity in nematodes and the detection rate of virulence factors did not corresponding to the virulence in nematodes, indicating complex pathogenicity mechanisms. We approved that C. elegans is a fast and convenient detection model for pathogenic bacteria virulence examinations.

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“…Diarrhoeagenic Escherichia coli (DEC) has been found in surface water used to irrigate food products (Canizalez-Roman et al, 2019) and from cow's milk, cheese and dairy cattle farm environments (Rios et al, 2019). It has also been found in pigs (Yang et al, 2019), cattle, poultry, diarrhoeic patients (Parvej et al, 2020) and so on. This means that DEC exists not only in the environment, but is also pathogenic for several animal species and for humans.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and virulence gene profiles of Escherichia coli from diarrhoeal rabbits in Sichuan Province, China

Yang

Wang²,

Geng³

et al. 2022

World Rabbit Sci.

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Escherichia coli (E. coli) can cause diarrhoea in a wide range of hosts. Moreover, some trains with high virulence and drug resistance pose a serious threat to public health and livestock products. Diarrhoea caused by E. coli outbreaks in rabbitries result in serious economic losses. The aim of this study was to investigate the distribution of virulence genes and molecular genetic characteristics of E. coli from diarrhoeal rabbits in the main rearing areas of Sichuan province, China in 2015-2017. In total, 39 E. coli isolates were identified and undivided divided into 17 sequence types by multilocus sequence typing (MLST) and grouped in 22 clusters by pulsed-field gel electrophoresis. Polymerase chain reaction tests detected 6 virulence genes: eae (41.0%), ler (41.0%), ral (33.3%), afr2 (10.3%), irp2 (15.4%) and astA (7.7%) of the tested 17 virulence genes identifying 16 enteropathogenic E. coli (EPEC) isolates. The main sequence types U328, ST328 and ST20 carried rabbit EPEC associated virulence genes (eae, ler, ral and afr2). The results showed that the distribution of virulence genes varied by year and area; genotype had major types in local rearing areas but was of high diversity in Sichuan province.

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Prevalence of Virulence Genes of Diarrheagenic <i>Escherichia coli</i> in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh

Cited by 6 publications

References 31 publications

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

Immunological pathogenesis of Bovine E. coli infection in a model of C. elegans

Genetic diversity and virulence gene profiles of Escherichia coli from diarrhoeal rabbits in Sichuan Province, China

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