Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of<i>Escherichia coli</i>Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire

Ollo, Kambiré; Adingra, A.A.; Yao, Konan Mathurin; Koffi-Névry, Rose

doi:10.1155/2017/9532170

Cited by 22 publications

(18 citation statements)

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“…Escherichia coli is a commensal bacteria found in the gastrointestinal tract of human and animals. Its ability to acquire virulence genes has originated strains that cause serious gastrointestinal infections in humans as well as animals (1). E. coli pathogenic strains are responsible for ∼56 million cases of diarrhea causing 0.2 million annual deaths worldwide, most of it in children between 2 and 5 years of age (2).…”

Section: Introductionmentioning

confidence: 99%

Captive Green Iguana Carries Diarrheagenic Escherichia coli Pathotypes

et al. 2020

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The green iguana appears to be a carrier for bacteria causing gastrointestinal infections in humans. The presence of diarrheagenic E. coli (DEC) pathotypes, however, has not been studied in this reptile. The aim of the current work was to investigate the prevalence of DEC in the intestines of 240 captive green iguanas, their phylogenetic groups, and the antibiotic susceptibility profile. E. coli strains were isolated from 41.7% (N = 100/240) of the intestinal content of green iguanas. DEC strains was identified in 25.9% of the screened population and were detected in the majority (62%, p = 0.009) of those reptiles carrying E. coli strains. Among DEC strains, STEC strains carrying the stx1 gene were the most prevalent pathotype isolated (38.7%), followed by EAEC and ETEC (27.4% each). Genetic markers of DEC strains belonging to the EHEC pathotype were not detected. More than a half of DEC strains were classified into the Clade I-II phylogroup (64.5%), followed by the phylogroup A (14.5%). The antibiotic susceptibility method demonstrated that a high proportion of DEC strains were resistance, or non-susceptible, to carbenicillin, amikacin, and ampicillin. We conclude that the green iguana kept in captivity is a carrier of DEC strains bearing resistance to first-line antibiotics, including penicillins. Given the increase presence of the green iguana in Latin American households, these reptiles represent a potential source of transmission to susceptible humans and therefore a potential source of gastrointestinal disease.

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Section: Introductionmentioning

confidence: 99%

Captive Green Iguana Carries Diarrheagenic Escherichia coli Pathotypes

et al. 2020

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“…DNA microarrays are collections of short oligonucleotide probes, representing thousands of genes, attached to a substrate (microarray) (Karakach et al 2010). Control samples and the samples to be evaluated are used.…”

Section: Microarray Platformmentioning

confidence: 99%

“…; Kambire et al . ; Kim and Lee ). Due to the importance of fish (as food) as carriers of some of these pathogens, the study of this food matrix is highlighted in a public health context.…”

Section: Introductionmentioning

confidence: 96%

“…In both marine and freshwater seafood, the intestinal microbiota comprises bacteria belonging to the Vibrio, Aeromonas, Pseudomonas, Plesiomonas, Acinetobacter, Clostridium, Bacteroides, Micrococcus, Flavobacterium genera and genera belonging to the Enterobacteriaceae family (Nayak 2010). Some of these bacteria, such as Vibrio, Aeromonas, Clostridium and enterobacteria, can cause diseases in humans, and are, therefore, considered pathogenic bacteria (Heitmann et al 2005;Iwamoto et al 2010;Kadariya et al 2014;Angelo et al 2015;Letchumanan et al 2015a;Stratev and Odeyemi 2016;Baron et al 2017;Elbashir et al 2017;Kambire et al 2017;. Due to the importance of fish (as food) as carriers of some of these pathogens, the study of this food matrix is highlighted in a public health context.…”

Section: Introductionmentioning

confidence: 99%

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Application of molecular tools to elucidate the microbiota of seafood

2018

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The aim of this review is to present the methodologies currently applied to identify microbiota and pathogens transmitted to humans through seafood consumption, focusing on molecular techniques and pointing out their importance, advantages, disadvantages and applicability. Knowledge of available techniques allows researchers to identify which technique best fits their expectations. With such discernment, it will be possible to infer which disadvantages will be present and, therefore, not interfering with the final result. Two methodologies can be employed for this purpose, dependent and independent cultures. However, the dependent culture has certain limitations that can be solved through the independent cultivation techniques, such as PCR, PFGE and NGS, especially through the sequencing of the 16S rRNA region, providing a complete view of microbial diversity. These have revolutionized microbiological knowledge, mainly because they allow for the identification of uncultivable micro-organisms, which represent a substantial portion of total micro-organisms, making it possible to elucidate not yet described taxa which may display pathogenic potential, besides quantifying microbial communities, microbiota genetics, translated proteins and produced metabolites. In addition, transcriptomic and metabolomic techniques also allow for the evaluation of possible impacts that microbial communities may create in their environment, as well as the determination of potential pathogenicity to humans.

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“…Escherichia coli is a commensal bacteria found in the gastrointestinal tract of human and mammals. Its ability to acquire virulence genes has originated strains that cause serious gastrointestinal infections in humans as well as animals (1). E. coli pathogenic strains are responsible for approximately 56 million cases of diarrhea causing 0.2 million annual deaths worldwide, most of it in children between 2 and 5 years of age (2).…”

Section: Introductionmentioning

confidence: 99%

Captive green iguana is a reservoir of diarrheogenicEscherichia colipathotypes

Bautista-Trujillo

Gutiérrez-Miceli

Mandujano-García

et al. 2019

Preprint

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20The green iguana appears to be a reservoir for bacteria causing gastrointestinal infections in 21 humans. The presence of diarrheogenic E. coli (DEC) pathotypes , however, has not been 22 studied in this reptile. The aim of the current work was to investigate the prevalence of 23 DEC in the intestines of 240 captive green iguanas, their phylogenetic groups, and the 24 antibiotic susceptibility profile. E. coli strains were isolated from 41.7% of the intestinal 25 content of green iguanas. DEC strains was identified in 25.9% of the screened population 26 and were detected in the majority (62%, p=0.009] of those reptiles carrying E. coli strains. 27Among DEC strains, STEC strains carrying the stx1 gene were the most prevalent 28 pathotype isolated (38.7%), followed by EAEC and ETEC (27.4% each). Genetic markers 29 of DEC strains belonging to the EHEC pathotype were not detected. More than a half of 30 DEC strains were classified into the Clade I-II phylogroup (64.5%), followed by the 31 phylogroup A (14.5%). The antibiotic susceptibility method demonstrated that a high 32 proportion of DEC strains were resistance, or non-susceptible, to carbenicillin, amikacin, 33 and ampicillin (85, 74, and 66%, respectively). We conclude that the green iguana kept in 34 captivity is a reservoir of DEC strains bearing resistance to first-line antibiotics, including 35 penicillins. Given the increase presence of the green iguana in Latin American households, 36 these reptiles represent a potential source of transmission to susceptible humans and 37 therefore a potential source of gastrointestinal disease. 38 Importance 39 Latin-American countries present a high burden of diarrheal disease. In this part of the 40 world, besides common pathogens, diarrheal diseases are also caused by pathogenic types 41 of E. coli referred as "diarrheagenic E. coli" (DEC). While inhabitants of Latin American 42 3countries suffer of self-limiting diarrhea when infected with DEC, a main target of these 43 strains are tourists from developed countries who are not exposed to DEC strains. Efforts 44 are in place to decrease the burden of DEC-associated diarrheal disease. As such, this study 45 investigated a potential reservoir of DEC strains that had been underestimated, the green 46 iguana. These reptiles are very common in Latin American households and are found 47 virtually everywhere in Mexico, Central and South America. We found that 25% of green 48 iguanas carried DEC strains in their intestines. We also demonstrated a high prevalence of 49 antibiotic resistance in these strains, posing a potential risk to humans. 50 51 Key words 52 Diarrheagenic Escherichia coli, prevalence, Iguana, Chiapas, antimicrobial resistance, 53 Zoonotic. 54 55 56 Escherichia coli is a commensal bacteria found in the gastrointestinal tract of 57 human and mammals. Its ability to acquire virulence genes has originated strains that cause 58 serious gastrointestinal infections in humans as well as animals (1). E. coli pathogenic 59 strains are responsible for approxim...

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Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes ofEscherichia coliIsolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire

Cited by 22 publications

References 36 publications

Captive Green Iguana Carries Diarrheagenic Escherichia coli Pathotypes

Captive Green Iguana Carries Diarrheagenic Escherichia coli Pathotypes

Application of molecular tools to elucidate the microbiota of seafood

Captive green iguana is a reservoir of diarrheogenicEscherichia colipathotypes

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