Prevalence of Vibrio parahaemolyticus in oyster and clam culturing environments in Taiwan

Yu, Wei-Ting; Jong, Koa-Jen; Lin, Yu‐Liang; Tsai, Shing-en; Tey, Yao Hsien; Wong, Hin-Chung

doi:10.1016/j.ijfoodmicro.2012.11.002

Cited by 72 publications

(68 citation statements)

References 42 publications

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“…is one of the major factors limiting the development of the marine economy. Among them, Vibrio parahaemolyticus has become a primary causative factor of food-borne disease outbreaks in coastal countries worldwide (Zarei et al 2012), including China, Japan, India, Thailand, Australia, and the United States (Wu et al 2014;Raghunath et al 2008;Iwamoto et al 2010;WHO/FAO 2011;Yu et al 2013): this affects the safety of seafood and the health of consumers. Vibrio parahaemolyticus is a natural bacterium in marine and estuarine environments, the animals carrying V. parahaemolyticus could be the principal vehicle transmitting the pathogenic bacterium to humans, including aquatic farmed animals (Griffitt et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Liu

Wang

Lǐ

et al. 2015

World J Microbiol Biotechnol

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Acute gastroenteritis caused by pathogenic Vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. Using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. In this study, a total of 249 isolates were isolated from four types of seafood (Litopenaeus vannamei, Oratosquilla oratoria, Mactra veneriformis and Portunus trituberculatus) and coastal sediment from Liaodong Bay in the Bohai Sea, China with five different separation agars. The most isolates came from the sample of coastal sediment and on agar of 2216E, which accounted for 36.14 and 54.62 % respectively. Twenty-four among 249 isolates displayed direct antimicrobial activity to V. parahaemolyticus with spot inoculation. Sixteen active isolates were selected for extracellular antimicrobial activity using the Oxford cup method. Only strains of B16 and J7 showed extracellular antimicrobial activity and were identified as Bacillus pumilus and Bacillus mojavensis respectively based on the physiological identification and 16S rRNA sequence analysis. Both of the strains B16 and J7 exhibited extracellular hydrolytic enzyme activity and antagonism against more than one indicator bacteria in vitro, which indicates that the two strains have broad potential application as suitable probiotic candidates in aquaculture while B. mojavensis was first reported to inhibit pathogenic Vibrio spp. in vitro. There is no particular trait as to antagonism of B. pumilus B16 or B. mojavensis J7 to Gram-positive or Gram-negative indicator bacteria.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Liu

Wang

Lǐ

et al. 2015

World J Microbiol Biotechnol

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“…Various studies have been carried out on seafood and findings concerning the distribution of pathogenic Vibrio in shellfish and fish are well documented (Gopal et al, 2005;Roque et al, 2009;Terzi et al, 2009;Collin and Rehnstam-Holm, 2011;Yu et al, 2013). In China, freshwater catfish, such as yellow catfish (Pelteobagrus fulvidraco); southern catfish (Silurus soldatovi meridionalis, Chen); Zhengchuan catfish (Silurus soldatovi meridionalis, Chen ♂ × Silurus asotus ♀); brown bullhead catfish (Ictalurus nebulosus); and channel catfish (Ictalurus punctatus), are the most widely cultured catfish.…”

Section: Introductionmentioning

confidence: 99%

Isolation and antibiotic susceptibility profile of Vibrio cholerae isolated from catfish (Pangasius hypophthalmus)

Norshafawati¹,

Noorlis²,

Kuan³

et al. 2017

Food Res.

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To date, cholera has cycle the world seven times through the seven pandemic cycles that has affected tens of millions of people. The objective of this study was to determine the presence and density as well as the antibiotic resistance profile of Vibrio cholerae isolated from catfish (Pangasius hypohthalamus). From the combination of the Most Probable Number-Polymerase Chain Reaction-plating on TCBS agar methods, V. cholerae was detected in 32 samples and V. cholerae O139 was detected in 7 samples, with a density ranging between <3.0 to 75.0 MPN/g and <3.0 to 9.3 MPN/g respectively. The results obtained in this study indicate that V. cholerae will continue to be a major healthcare burden, as the pathogen can be transferred from the aquatic environment to the catfish and the consumption of catfish by humans will present a route of exposure to V. cholerae. Proliferation of antibiotic and multiantibiotic resistant bacteria is a public health threat worldwide. Results of antimicrobial susceptibility investigation of V. cholerae isolates collected from catfish reported in this study will establish an important baseline data. All the V. cholerae isolates were multiantibiotic resistant towards the ten antibiotics tested, including 6 isolates that were resistant to all ten antibiotics and 38 antibiotic resistance patterns. The MAR index values of 0.2 to 1.0 indicate that the isolates were exposed to high risk sources in the environment. Taken together, the information on the prevalence and antibiotic resistance of V. cholerae indicate that catfish consumption presents a potential risk to human health and highlight the need for ongoing epidemiological and antimicrobial resistance surveillance.

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“…In the coastal cities of China, more than 60% of seafood tested positive for bacterial pathogens [7,8]. The fact that pathogens could be maintained in oysters for at least one month [9] imposes a huge risk for human health [3,4].…”

Section: Introductionmentioning

confidence: 99%

http://www.omicsonline.org/open-access/overcoming-pseudomonas-aeruginosa-resistance-caused-by-glycocalyx-with-tobracef-1948-5948.1000145.php?aid=25925

Dai¹

2014

J Microb Biochem Technol

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Oysters are filter feeders that bioaccumulate bacteria in water while feeding. To evaluate the bacterial genomic DNA extracted from retail oyster tissues, including the gills and digestive glands, four isolation methods were used. Genomic DNA extraction was performed using the Allmag™ Blood Genomic DNA (Allrun, Shanghai, China), the MiniBEST Bacterial Genomic DNA Extraction kits (Takara, Dalian, China), and the phenol-chloroform and boiling lysis methods. The concentration of the genomic DNA was measured using a spectrophotometer. The purity of the genomic DNA was evaluated by PCR amplification of 16S rDNA followed by determining the cloning efficiency of the amplicon into the pMD19-T vector. Furthermore, the bacterial DNA quality was also evaluated by PCR assays using a pair of species-specific primers for Vibrio parahaemolyticus. Our results showed that the two commercial kits produced the highest purity of DNA, but with the lowest yields. The phenol-chloroform method produced the highest yield although it was time-consuming. The boiling lysis method was simple and cost effective; however, it was only suitable to isolate genomic DNA from bacteria present in retail samples following an enrichment step. The two commercial kits were good candidates for genomic DNA extraction from retail oyster tissues without enrichment.

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Prevalence of Vibrio parahaemolyticus in oyster and clam culturing environments in Taiwan

Cited by 72 publications

References 42 publications

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Isolation and antibiotic susceptibility profile of Vibrio cholerae isolated from catfish (Pangasius hypophthalmus)

http://www.omicsonline.org/open-access/overcoming-pseudomonas-aeruginosa-resistance-caused-by-glycocalyx-with-tobracef-1948-5948.1000145.php?aid=25925

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