Prevalence of Ureaplasma Urealyticum, Mycoplasma Hominis, Chlamydia Trachomatis Infections, and Semen Quality in Infertile and Fertile Men in China

Liu, Junjie; Wang, Quanxian; Ji, Xiaofei; Guo, Shang; Dai, Yanpeng; Zhang, Zhan; Jia, Longfei; Shi, Ying; Tai, Shuhong; Lee, Yushan

doi:10.1016/j.urology.2013.11.009

Cited by 63 publications

(60 citation statements)

References 18 publications

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“…The role of C. trachomatis infection on semen parameters in male infertility is controversial. In fact, a large number of studies have suggested that positive markers for Chlamydia infection are not associated with altered sperm parameters [18], [19], [46], [54]–[55]. Others, however, have found that Chlamydia infection correlates with reduced sperm motility [34]–[56], increased proportion of sperm abnormalities [57], significant reductions in semen density, sperm morphology, and viability [58] and increased likelihood of leukocytospermia [34].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Chlamydia trachomatis and Other Sexually Transmitted Bacteria in Semen of Male Partners of Infertile Couples in Tunisia: The Effect on Semen Parameters and Spermatozoa Apoptosis Markers

et al. 2014

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This study was undertaken to determine the prevalence of Chlamydia trachomatis, Mycoplasmas, and Ureaplasmas in semen samples of the male partners of infertile couples and to investigate whether Chlamydia trachomatis could initiate apoptosis in human spermatozoa. A total of 85 males partners of infertile couples undergoing routine semen analysis according to World Health Organization guidelines were included. Specimens were examined for the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvum by Real time PCR (qPCR). Semen specimens were analysed for the appearance of apoptotic markers (sperm DNA fragmentation, activated caspase 3 levels, mitochondrial membrane potential (ΔΨm)) using flow cytometry. C. trachomatis, N. gonorrhoeae, U. urealyticum, M genitalium were detected in semen samples of 13 (15.2%), 5 (5.8%), 5 (5.8%) and 3 (3.5%) male partners of infertile couples, respectively. M. hominis and U. parvum were detected in semen sample of only one patient (1.1%). The semen of infertile men positive for C. trachomatis showed lower mean of semen count and lower rapid progressive motility (category [a]) of spermatozoa compared to uninfected men with statistically significances (p = 0.02 and p = 0.04, respectively). Flow cytometry analyses demonstrated a significant increase of the mean rate of semen with low ΔΨm and caspase 3 activation of infertile men positive for C. trachomatis compared to uninfected men (p = 0.006 and p = 0.001, respectively). DNA fragmentation was also increased in sperm of infertile men positive for C. trachomatis compared to uninfected men but without statistical significances (p = 0.62). Chlamydial infection was associated to loss of ΔΨm and caspase 3activation. Thus, C. trachomatis infection could be incriminated in apoptosis induction of spermatozoa. These effects may explain the negative direct impact of C. trachomatis infection on sperm fertilizing ability.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Chlamydia trachomatis and Other Sexually Transmitted Bacteria in Semen of Male Partners of Infertile Couples in Tunisia: The Effect on Semen Parameters and Spermatozoa Apoptosis Markers

et al. 2014

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“…trachomatis is one of the most common pathogens that cause sexually transmitted infections (STIs). 1 Although as much as two-thirds of women and 50% of men who are infected with this organism develop asymptomatic infections, 2 in the remaining population, this bacterium can cause a broad spectrum of genitourinary infections, such as non-and post-gonococcal urethritis, epididymitis and prostatitis in males 3 and cervicitis, salpingitis and pelvic inflammatory disease (PID) in females, which particularly in the chronic form may lead to ectopic pregnancy or exert negative effects on fertility. Furthermore, in vitro co-incubation of C. trachomatis with human spermatozoa can impair sperm motility and kill spermatozoa likely because of the bacterium lipopolysaccaride, 4 although the role of this pathogen in male infertility remains controversial.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of genitalChlamydia trachomatisin Iran: a systematic review and meta-analysis

Ahmadi

Mirsalehian

Bahador

2015

Pathogens and Global Health

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Objective: To determine the overall prevalence of Chlamydia trachomatis in Iranian males and females and to find out the effect of this bacterium on fertility potential and its association with urogenital symptoms. Methods: We searched both English and Persian electronic databases using keywords 'Chlamydia', 'Chlamydia trachomatis', 'prevalence', 'incidence', 'frequency', 'epidemiology' and 'Iran'. Finally, after some exclusion, 34 studies from different regions of Iran were included in our study, and a meta-analysis was performed to determine pooled prevalence estimates for each group. Results: C. trachomatis prevalence for women and men was high and ranged from 0 to 32.7% and 0 to 23.3%, respectively (95% CI). The pooled prevalence of the bacterium in the female population was 12.3% (95% CI: 10.6-14.2%) and in men was 10.9% (95% CI: 7.6-15.4%). A high level of heterogeneity was seen for both men (I 2 5 77.4%; P v 0.001) and women (I 2 5 77.5%; P v 0.001); but in men and not in women, some evidence for publication bias was observed [Egger's test (twotailed P 5 0.013); Begg's test (two-tailed P 5 0.025)]. In females analysis of symptomatic/infertile group with asymptomatic/fertile group in females, the overall OR was above 1 and the overall P-value was below zero. Conclusions: This bacterium may play a role in female infertility or be associated with clinical manifestations; thus, planning national programmes for adequate diagnosis of genital infections caused by this pathogen is necessary. Furthermore, screening strategies, particularly for asymptomatic individuals, and treatment of infected people can reduce consequent complications.

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“…Fakat hedeflenen hastalarda çalışılabileceğini belirtilmiştir [22]. Çin ve Kuveyt toplumlarında yapılan çalışmalarda enfekte semenle enfekte olmayan semen arasında infertilite açısından anlamlı fark bulunmamıştır [24,25]. Çalışmamızda ise herhangi bir etyolojisi bulunmayan Van yöresindeki hastalarımızın sonuçla-rı anlamlı olmasına rağmen (p< 0,05), çalışma gurubundaki olgu sayısının azlığı, infertil olgularda antibiyotik tedavisinin etkinliği hakkında, bilimsel yargıda bulunulmasını engeller niteliktedir.…”

Section: Discussionunclassified

Prevalence of Ureaplasma and Mycoplasma in Infertile Men in Van Region and Effects to Semen Parameters

Taken¹

2015

Ann Clin Anal Med

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ÖzetGiriş: Van yöresinde nedeni belirlenemeyen primer infertilite olgularının semen kültürlerinde Ureaplasma urealyticum(UU) ve Mycoplasma hominis(MH) sıklığı ve tedavinin sperm parametrelerine etkisinin araştırılması amaçlandı. Gereç ve Yöntem: 106 olgunun dahil edildiği bu çalışmada, infertil gurup (41 olgu), alt üriner sistem semptomları olan gurup (33 olgu) ve kontrol gurubu (32 olgu) olmak üzere 3 gurup oluşturuldu. Çalışma gurubundaki infertil olguların özgeçmişlerinde varikosel, testis torsiyonu, hidrosel, inmemiş testis öyküsü ve hormonal nedenler yoktu. Kontrol gurubundaki olgular, infertil ve alt üriner sistem semptomu olmayan olgulardan oluşturuldu. İnfertil gurupta, kültür pozitif olguların semen parametreleri tedavi öncesi ve sonrası incelendi. Mycoplasma türlerinin ayırımı Biomerieux® Mycoplasma IST 2 (RCS Lyon-France) kitleriyle yapıldı. Sperm sayımı Makler sayım kamarası ile yapıldı (Self Medical Industries, Haifa, Israel). Bulgular: İnfertil guruptaki 17 olguda (% 41,5) UU, 3 olguda (% 7,5) MH izole edildi. AÜSS'lu gurupta UU izole edilen olgu sayısı 15 (%45,5), MH izole edilen olgu sayısı 3 (% 9,1) idi. Kontrol gurubundaki olgularda MH izole edilmezken, 6 olguda (% 18,8) UU izole edildi . İnfertil guruptaki kültür pozitif 3 (% 15) olgunun sperm sayısı < 15 milyon iken kültür negatif olgularda ise 10 olgunun (%50) sperm sayısı 15 milyonun altındaydı ( p< 0,025). Tedavi öncesi kültür pozitif 14 olgunun (% 70) sperm motilitesi % 40'nin altındaydı. Tedavi sonrasında ise 5 olgunun (% 25) sperm motilitesi < % 40 idi. Motilitede anlamlı artış olduğu izlendi. Tedavi sonrası, infertil guruptaki kültür pozitif 20 olgunun (% 49) 4'ünün (% 20) eşinde gebelik sağlandığı görüldü (p<0,025) .Tartışma: Antibiyotik tedavisinin nedeni belirlenmemiş kültür pozitif infertil olgularda sperm motilitesine olumlu etki yaparak tedaviye olumlu katkı sağladığı izlenmiştir. Anahtar Kelimelerİnfertilite/Erkek; U.Urealyticum; M.Hominis; Semen Parametreleri Abstract Aim: The purpose of this study was to assess the prevalence of Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) in semen cultures of cases with primary infertility in the Van Province, and also to determine the effect of therapy on sperm parameters. Material and Method: The study included 106 individuals divided into three groups: The infertile group (41 cases), the group with lower urinary tract symptoms (33 cases), and the control group (32 cases). The patients in the infertile group had no history of varicocele, testicular torsion, hydrocele, undescended testis, and hormonal disorders. The control group included cases without infertility and lower urinary tract symptoms. The parameters of culture-positive cases in the infertile group were determined before and after therapy. The identification of Mycoplasma species was made using the Biomerieux® Mycoplasma IST 2 (RCS LyonFrance) kit. The sperm count was carried out with the Makler counting chamber (Self Medical Industries, Haifa, Israel). Results: In the infertile group, UU was isolated from 17 and MH wa...

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Prevalence of Ureaplasma Urealyticum, Mycoplasma Hominis, Chlamydia Trachomatis Infections, and Semen Quality in Infertile and Fertile Men in China

Cited by 63 publications

References 18 publications

Molecular Detection of Chlamydia trachomatis and Other Sexually Transmitted Bacteria in Semen of Male Partners of Infertile Couples in Tunisia: The Effect on Semen Parameters and Spermatozoa Apoptosis Markers

Molecular Detection of Chlamydia trachomatis and Other Sexually Transmitted Bacteria in Semen of Male Partners of Infertile Couples in Tunisia: The Effect on Semen Parameters and Spermatozoa Apoptosis Markers

Prevalence of genitalChlamydia trachomatisin Iran: a systematic review and meta-analysis

Prevalence of Ureaplasma and Mycoplasma in Infertile Men in Van Region and Effects to Semen Parameters

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