Prevalence of thermotolerant Campylobacter spp. in broilers at retail in the Czech Republic and their antibiotic resistance

Bardoň, Jan; Kolář, Milan; Karpíšková, Renata; Hricová, Kristýna

doi:10.1016/j.foodcont.2010.08.001

Cited by 21 publications

(18 citation statements)

References 28 publications

(29 reference statements)

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“…Analysis of Campylobacter species identified in the present study revealed that most of them were C. coli (58.9%) while C. jejuni was detected in the remaining 41.1% poultry meat samples. These findings are in contrast to data obtained by other authors who in similar samples detected mostly C. jejuni (Sallam 2007;Rozynek et al 2008;Bardon et al 2011;Anonymous 2012a). However, there are also some reports were C. coli was more predominant than C. jejuni in poultry meat (Kurincic et al 2005;Lynch et al 2011;Mackiw et al 2012).…”

Section: Discussioncontrasting

confidence: 99%

“…On the other hand, some EU MSs reported a relatively high level of resistance to erythromycin, e.g., 4% and 18% in Belgium for C. jejuni and C. coli, respectively or 39% of the Campylobacter isolates in the Netherlands (Anonymous 2012b). Furthermore, a relatively low level of resistance of the strains tested to streptomycin (22.8%) obtained during our study is similar to other data where the percentage of such strains was higher among C. coli than C. jejuni (McGill et al 2006;Bardon et al 2011). In the present investigation none of the Campylobacter strains was resistant to another antimicrobial from the aminoglycoside group -gentamicin.…”

Section: Discussionsupporting

confidence: 91%

“…As described in the EFSA report (Anonymous 2012b) resistance to these groups of antimicrobials is predominant among Campylobacter isolates of poultry meat origin in many EU MSs. However, other studies, especially those conducted in the Nordic countries, showed low resistance rates among these microorganisms isolated from chicken meat (Frediani-Wolf and Stephan 2003;Andersen et al 2006;Bardon et al 2011). Our data on the susceptibility of Campylobacter isolates to ciprofloxacin are similar to the results obtained in some other European countries, e.g.…”

Section: Discussionsupporting

confidence: 89%

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Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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ABSTRACT:The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

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Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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“…In several studies, poultry has been identified as an important risk factor for Campylobacter infection in humans (3,8,10,15,16). In the present study, the prevalence of these bacteria in poultry carcasses (62.1%) was similar to that described recently in Belgium (51.9%) in broilers at slaughter (15).…”

Section: Discussionsupporting

confidence: 89%

“…As indicated in many studies, the use of quinolones in veterinary practice has been associated with the emergence of CIP resistance in Campylobacter isolates of poultry and human origin (l, 3,5,9,22,26). In contrast, in countries where quinolones have not been used in animal production, a low prevalence of CIP-resistant strains has been noted (1,3,22,26). The high prevalence of resistance to quinolones among the Campylo bacter isolates in the present study may be a result of the way these antimicrobials are used in Poland, i.e., as therapeutic treatments in veterinary medicine.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Campylobacter spp. Isolated from Poultry Carcasses in Poland

Wieczorek

Kania

Osek

2013

Journal of Food Protection

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The purpose of the present study was to determine the prevalence of Campylobacter in poultry carcasses at slaughter in Poland. For the isolated strains, resistance to selected antibiotics and the associated genetic determinants were identified. A total of 498 Campylobacter isolates were obtained from 802 poultry samples during the 2-year study period. Strains were identified to species with the PCR method; 53.6% of the strains were Campylobacter jejuni and 46.4% were Campylobacter coli. A high percentage of the tested Campylobacter strains were resistant to ciprofloxacin and nalidixic acid (74.1 and 73.5%, respectively) followed by tetracycline (47.4%) and streptomycin (20.5%). Only one C. jejuni and two C. coli isolates were resistant to gentamicin. Seventy-nine (15.9%) of the 498 strains were resistant to three or more classes of antibiotics examined. Higher levels of resistance, irrespective of the antimicrobial agent tested, were found within the C. coli group. Almost all strains resistant to quinolones (99.5%) and to tetracycline (99.6%) carried the Thr-86-to-Ile mutation in the gyrA gene and possessed the tet(O) marker, respectively. All isolates resistant to erythromycin had the A2075G mutation in the 23S rRNA gene. These results reveal that poultry carcasses in Poland are a reservoir of potentially pathogenic and antimicrobial-resistant Campylobacter strains for humans, which may pose a public health risk.

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Campylobacter

Kaakoush

Mitchell

Man

2015

Molecular Medical Microbiology

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Prevalence of thermotolerant Campylobacter spp. in broilers at retail in the Czech Republic and their antibiotic resistance

Cited by 21 publications

References 28 publications

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Prevalence and Antimicrobial Resistance of Campylobacter spp. Isolated from Poultry Carcasses in Poland

Campylobacter

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