Prevalence of the infectious hypodermal and hematopoietic necrosis virus in shrimp (Penaeus vannamei) broodstock in northwestern Mexico

Mendoza–Cano, Fernando; Enríquez-Espinoza, Tania; Encinas‐García, Trinidad; Sánchez‐Paz, Arturo

doi:10.1016/j.prevetmed.2014.09.006

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…This was due to the limited number of specific pathogen-free (WSSV and PstDV-1) shrimp from Sonora. Epidemiologic studies have demonstrated a high estimated prevalence for PstDV-1 in broodstock of P. vannamei from hatcheries on the northwest of Mexico (up to 63%) (Mendoza-Cano et al 2016). Yet, we found significant differences among the properties of the proteasome of P. vannamei and C. crangon.…”

Section: Discussionmentioning

confidence: 99%

Proteasome properties of hemocytes differ between the whiteleg shrimp Penaeus vannamei and the brown shrimp Crangon crangon (Crustacea, Decapoda)

Götze

Saborowski

Martínez‐Cruz

et al. 2017

Cell Stress and Chaperones

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Crustaceans are intensively farmed in aquaculture facilities where they are vulnerable to parasites, bacteria, or viruses, often severely compromising the rearing success. The ubiquitin-proteasome system (UPS) is crucial for the maintenance of cellular integrity. Analogous to higher vertebrates, the UPS of crustaceans may also play an important role in stress resistance and pathogen defense. We studied the general properties of the proteasome system in the hemocytes of the whiteleg shrimp, Penaeus vannamei, and the European brown shrimp Crangon crangon. The 20S proteasome was the predominant proteasome population in the hemocytes of both species. The specific activities of the trypsin-like (Try-like), chymotrypsinlike (Chy-like), and caspase-like (Cas-like) enzymes of the shrimp proteasome differed between species. P. vannamei exhibited a higher ratio of Try-like to Chy-like activities and Caslike to Chy-like activities than C. crangon. Notably, the Chy-like activity of P. vannamei showed substrate or product inhibition at concentrations of more than 25 mmol L −1 . The K M values ranged from 0.072 mmol L −1 for the Try-like activity of P. vannamei to 0.309 mmol L −1 for the Cas-like activity of C. crangon. Inhibition of the proteasome of P. vannamei by proteasome inhibitors was stronger than in C. crangon. The pH profiles were similar in both species. The Try-like, Chy-like, and Cas-like sites showed the highest activities between pH 7.5 and 8.5. The proteasomes of both species were sensitive against repeated freezing and thawing losing~80-90% of activity. This study forms the basis for future investigations on the shrimp response against infectious diseases, and the role of the UPS therein.

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Section: Discussionmentioning

confidence: 99%

Proteasome properties of hemocytes differ between the whiteleg shrimp Penaeus vannamei and the brown shrimp Crangon crangon (Crustacea, Decapoda)

Götze

Saborowski

Martínez‐Cruz

et al. 2017

Cell Stress and Chaperones

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“…Of these 16/30 had problems while 14/30 did not, indicating that IHHNV was not significantly associated with problem ponds and that other causes should have been sought but were not. In addition, publications from the interval 2010 to 2020 that describe lack of significant problems linked to IHHNV were not included in the AAHC review (e.g., Hernández-Ruíz et al, 2020; Mendoza-Cano et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

No single PCR test is sufficient to determine parvovirus IHHNV presence in or impact on farmed shrimp production

Sritunyalucksana,

Sanguanrut,

Srisala

et al. 2023

Preprint

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The purpose of this study was to confirm evidence that the shrimp parvovirus IHHNV no longer has a significant negative economic impact on farmed shrimp production, and that it should be delisted as a pathogen by the World Organization for Animal Health (WOAH). Current published evidence for delisting is presented in the introduction, and experimental evidence is presented from 11 commercial ponds cultivating the giant tiger shrimpPenaeus monodonin Thailand. All 11 ponds gave positive PCR test results for IHHNV using 2 methods recommended for IHHNV diagnosis by WOAH. However, when an additional in-house “IHHNV Long-Amp method” (IHHNV-LA) was also used to amplify 90% of the 4 kb IHHNV genome sequence, only 4 of the 11 ponds gave positive PCR test results. Further tests using normal histopathological analysis for the presence of pathognomonic Cowdry A type inclusions (CAI),in situhybridization (ISH) and immunohistochemistry (IHC) could confirm IHHNV infections in only 2 of those 4 ponds. In addition, a positive detection of CAI alone was equivalent to ISH or IHC in confirming IHHNV infection after a positive test with any of the PCR methods used. In summary, the recommended WOAH PCR methods gave false positive test results for IHHNV infection with 9/11 ponds (82%). All 11 ponds gave profitable harvests despite the confirmation of IHHNV in 2 ponds, where it was accompanied by various additional pathogens, as were all 11 ponds. Unfortunately, according to current practice, positive PCR test results with the WOAH methods can lead to rejection of traded shrimp products without assurance that they are not false positive results arising from EVE. Our results, results from others and our analysis of a recent decision of WOAH to maintain IHHNV as a listed disease suggest that WOAH must reconsider IHHNV for de-listing.

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“…In Mexico, shrimp postlarvae with fast-growing and high-resistance characteristics are produced for the national aquaculture (Castillo-Juárez et al, 2015;Mendoza-Cano et al, 2014). Fishmeal and fish oil are the main ingredients for the formulated foods intended for aquaculture; however, these depend upon limited resources while posing a risk of overexploitation by fisheries (FAO, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary protein and genetic line of Litopenaeus vannamei on its hepatopancreatic microbiota

Martínez‐Porchas

Vargas‐Albores

Casillas-Hernández

et al. 2021

Sci. agric. (Piracicaba, Braz.)

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Host genetics and diet can exert an influence on microbiota and, therefore, on feeding efficiency. This study evaluated the effect of genetic line (fast-growth and high-resistance) in Pacific white shrimp (Litopenaeus vannamei) on the hepatopancreatic microbiota and its association with the feeding efficiency in shrimp fed with diets containing different protein sources. Shrimp (2.08 ± 0.06 g) from each genetic line were fed for 36 days with two dietary treatments (animal and vegetable protein). Each of the four groups was sampled, and the hepatopancreatic metagenome was amplified using specific primers for the variable V4 region of the 16S rRNA gene. The PCR product was sequenced on the MiSeq platform. Nineteen bacterial phyla were detected, of which Proteobacteria was the most abundant (51.0-72.5 %), Bacteroidetes (3.6-23.3 %), Firmicutes (4.2-13.7 %), Actinobacteria (1.9-12.1 %), and Planctomycetes (1.3-9.5 %). Diet was the most influential factor in the taxonomic composition of the microbiota, while genetic line was not a strong influential factor. The results suggest that the taxonomic profile of the bacteria colonizing shrimp hepatopancreas was determined by the diet consumed, similar to what occurs in the intestine. Shrimp in the fast-growth line had greater feeding efficiency regardless of the diet supplied. Finally, the results suggest that Proteobacteria influenced (p < 0.05) the feeding efficiency of shrimp fed with a vegetable diet. Nevertheless, further studies are required to explore how shrimp genetic line-diet interaction influences microbiota for probiotic development and functional food formulation for farmed shrimp according to the genetic line.

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Prevalence of the infectious hypodermal and hematopoietic necrosis virus in shrimp (Penaeus vannamei) broodstock in northwestern Mexico

Cited by 10 publications

References 19 publications

Proteasome properties of hemocytes differ between the whiteleg shrimp Penaeus vannamei and the brown shrimp Crangon crangon (Crustacea, Decapoda)

Proteasome properties of hemocytes differ between the whiteleg shrimp Penaeus vannamei and the brown shrimp Crangon crangon (Crustacea, Decapoda)

No single PCR test is sufficient to determine parvovirus IHHNV presence in or impact on farmed shrimp production

Effect of dietary protein and genetic line of Litopenaeus vannamei on its hepatopancreatic microbiota

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