Prevalence of tetracycline resistance genes in Greek seawater habitats

Nikolakopoulou, Theodora L.; Giannoutsou, Eleni; Karabatsou, Adamandia A.; Karagouni, Amalia D.

doi:10.1007/s12275-008-0080-8

Cited by 18 publications

(16 citation statements)

References 42 publications

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“…Our findings are consistent with the results of published studies [29]. The results of our previous study [30] and other authors' findings [31,32] testify to the high drug resistance of TET R and OTC R bacteria. According to Hall et al [33], resistance to a single antibiotic could lead to selective resistance to other drugs and, ultimately, multiple-drug resistance.…”

Section: Discussionsupporting

confidence: 93%

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Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

et al. 2017

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Abstract. The objective of this study was to monitor changes (amplification or attenuation) in antibiotic resistance during wastewater treatment based on the ecology of tetracycline-resistant bacteria. The untreated and treated wastewater were collected in four seasons. Number of tetracycline-(TET R ) and oxytetracycline-resistant (OTC R ) bacteria, their qualitative composition, minimum inhibitory concentrations (MICs), sensitivity to other antibiotics, and the presence of tet(A, B, C, D, E) resistance genes were determined. TET R and OTC R counts in untreated wastewater were 100 to 1000 higher than in treated effluent. OTC R bacterial counts were higher than TET R populations in both untreated and treated wastewater. TET R isolates were not dominated by a single bacterial genus or species, whereas Aeromonas hydrophila and Aeromonas sobria were the most common in OTC R isolates. The treatment process attenuated the drug resistance of TET R bacteria and amplified the resistance of OTC R bacteria. In both microbial groups, the frequency of tet(A) gene increased in effluent in comparison with untreated wastewater. Our results also indicate that treated wastewater is a reservoir of multiple drug-resistant bacteria as well as resistance determinants which may pose a health hazard for humans and animals when released to the natural environment.

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Section: Discussionsupporting

confidence: 93%

“…According to Tao et al [19], around 30% bacteria have at least two genes encoding tetracycline resistance. Similar findings were reported in studies of other bacterial species by Akinbowale et al [15], Henriques et al [31] and Nikolakopoulou et al [32].…”

Section: Discussionsupporting

confidence: 90%

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

et al. 2017

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“…Dang et al (2007) concluded that there is risk of multidrug-resistant bacteria contamination in mariculture environments, and marine Vibrio and Pseudoalteromonas species can serve as reservoirs of resistance determinants. Nikolakopoulou et al (2008) studied the presence of selected tet genes in different Greek seawater habitats, originated from wastewater treatment facilities, fish farm, and coastal environments. The molecular methods employed included assessment of the presence of twelve tet gene clusters by PCR, followed by hybridization with specific probes, in total DNA extracted from the habitats.…”

Section: Frequently Detected Tet(o)mentioning

confidence: 99%

“…Isolated plasmids were shown to carry tet(A), tet(C), tet(E), and tet(K) genes. Nikolakopoulou et al (2008) concluded that tet genes are widespread in the seawater habitats studied and are often encoded on conjugative broad-host-range plasmids.…”

Section: Frequently Detected Tet(o)mentioning

confidence: 99%

Molecular Detection of Resistance and Transfer Genes in Environmental Samples

Grohmann

Arends

2011

Environmental Protection Strategies for Sustainable Development

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“…Antibiotics used in therapy and agriculture accumulate in the environment and contaminate aquatic habitats where they can exert their selective pressure on the native flora (2, 4, 7). Importantly, oxytetracyclines are frequently used in aquaculture (21), and tetracycline resistance genes, including tet(L), have been described in many aquatic environments (1,15,18).…”

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confidence: 99%

Tetracycline Resistance-Encoding Plasmid from Bacillus sp. Strain #24, Isolated from the Marine Sponge Haliclona simulans

Phelan

Clarke

Morrissey

et al. 2011

Appl Environ Microbiol

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Knowledge of the nature of resistance determinants in natural habitats is fundamental to increasing our understanding of the development of antibiotic resistance in clinical settings. Here we provide the first report of a tetracycline resistance-encoding plasmid, pBHS24, from a marine sponge-associated bacterium, Bacillus sp. strain #24, isolated from Haliclona simulans.The increased selection pressure created by the overuse and misuse of antibiotics in clinical and agricultural settings has undoubtedly driven the evolution and spread of resistance determinants (8, 11). While many resistance genes are believed to have their origin in natural ecosystems, their abundance, nature, and ecological role in such settings remain relatively obscure (2). It is nevertheless accepted that this knowledge is fundamental to tackle the ingenious ways that bacteria curtail the effectiveness of antimicrobial agents.Research on marine sponge-associated bacteria has increased exponentially in recent years, but this has focused predominantly on phylogeny and on the search for novel bioactive compounds (13,22). While much data are available on the microbial ecology of marine sponges (12, 22), comparatively little is known about the incidence and diversity of antibiotic resistance determinants and the often associated mobile elements among these bacteria.We have recently characterized the spore-forming population of the marine sponge Haliclona simulans, collected from Gurraig Sound, Kilkieran Bay, Galway, Ireland. One of the isolates was identified by partial 16S rRNA gene sequencing as a Bacillus sp., with 96% sequence identity to its closest relative, Bacillus lehensis. Bacillus sp. strain #24 was unable to grow in SYP (12) and LB media unless supplemented with either 50% artificial seawater (12) or 2% NaCl and was resistant to tetracycline and erythromycin (MICs of 75 g ml Ϫ1 and 3 mg ml Ϫ1 , respectively. MIC values reported in this study are defined as the minimal concentration of antibiotic able to inhibit the growth of the strains when spotted on Muller-Hinton medium plates containing increasing concentrations of the antibiotic). Screening of isolate #24 for plasmid DNA (6) was positive, with multiple bands visible on agarose gels. In order to ascertain its role in antibiotic resistance, total plasmid DNA purified from isolate #24 (QIAprep Spin miniprep kit optimized for Bacillus; Qiagen GmbH) was used to transform Bacillus subtilis 168 competent cells. This strain is sensitive to erythromycin and displays only a very low level of resistance to tetracycline (2 g ml Ϫ1 ) associated with the presence of a chromosomally carried tet(L) gene (9, 20). B. subtilis 168 is easily differentiated from isolate #24 by colony morphology (Fig. 1). A large number of transformants were obtained on LB medium-tetracycline plates, while no colonies grew on those containing erythromycin (both at 5 g ml Ϫ1 ). This implied that tetracycline resistance was associated with plasmid DNA, while erythromycin resistance was probably chromosomally encoded. A...

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Prevalence of tetracycline resistance genes in Greek seawater habitats

Cited by 18 publications

References 42 publications

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

Molecular Detection of Resistance and Transfer Genes in Environmental Samples

Tetracycline Resistance-Encoding Plasmid from Bacillus sp. Strain #24, Isolated from the Marine Sponge Haliclona simulans

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