Prevalence of tet gene and complete genome sequencing of tet gene-encoded plasmid (pAHH01) isolated from Aeromonas species in South Korea

Abstract

Aims: To investigate the tetracycline resistance related to tet genes in Aeromonas isolates collected from water and diseased fish in South Korea. Methods and Results: A total of 34 Aeromonas strains were examined for their susceptibility to tetracycline using the minimum inhibitory concentration (MIC) assay, and the genetic determinants (tetA to E) were analysed. Among these strains, the tetA and tetE genes were predominant (tetA was found in six strains, and tetE was found in nine strains), and 15 strains were tetracycline‐resistant by the MIC assay. Additionally, the 8979‐bp plasmid that contains the tetE gene was fully sequenced. Conclusions: These data may be important with regard to the spread and persistence of tetracycline resistance genes in the bacterial populations that are present in aquaculture systems. Significance and Impact of the Study: Interestingly, no isolate has previously been shown to harbour three tet genes that are mediated by efflux systems, but the tetA, tetD and tetE genes were all isolated from one strain, which had the highest MIC value for tetracycline among the strains analysed in this study. We also investigated the full‐length plasmid that encoded the tetE gene from a tetracycline‐resistant strain.

Antibiotic resistance and virulence factors in mesophilic Aeromonas spp. from Czech carp fisheries

“…Our study showed that tetracycline resistance of Aeromonas spp. isolates from common carp was encoded mostly by the tet(E) gene which has been previously shown to be a dominant tetracycline resistance gene in aeromonads from fresh fish aquaculture (Nawaz et al 2006;Cizek et al 2010;Han et al 2012). Two isolates resistant to tetracycline that were negative for tet genes likely carry one of the less frequent tetracycline resistance determinants that were not tested in our study (Akinbowale et al 2006).…”

“…; Han et al . ). Two isolates resistant to tetracycline that were negative for tet genes likely carry one of the less frequent tetracycline resistance determinants that were not tested in our study (Akinbowale et al .…”

Insight into the mobilome of Aeromonas strains

“…In addition to the frequently occurring BHR plasmids that were discussed earlier (IncA/C, IncU, IncQ), plasmids belonging to IncFrepB, IncFIB, IncFIC and IncI groups were also observed in the genus Aeromona s (Han et al, 2012b ; Moura et al, 2012 ; Maravić et al, 2013 ). The study of Maravić et al ( 2013 ) found 40-kb conjugative plasmids described as narrow host range IncFIB group in 11 A. caviae strains isolated from Croatian mussels.…”

Genomics of high molecular weight plasmids isolated from an on-farm biopurification system

“…These results are in concordance with the taxonomic assignments of the plasmid-containing host bacteria from the BPS as determined by 16S rRNA gene sequencing 14 . Some of the reference plasmids encoding the identified Rep proteins, such as pCAR1 19 , pND6-1 20 , pSTY 21 , and pDK1 22 contain degradative genes, and others such as pMET-1 23 , RP4 24 , pAHH01 25 , pIE321 26 , and pTB11 27 contain antibiotic resistance genes. Identity values between the identified Rep proteins and the best BLASTp hit in the NCBI database ranged from 46% to 100% ( Table 1 ).…”