Prevalence of Subclinical Mastitis in Small Ruminants and Role of Staphylococcus Species in Such Infection

Haggag, Yasser N.; Nossair, Mohammad; Habib, Heba M.; Naggar, Ahmad L. El; Abdallah, Mona; Farag, Heba E.

doi:10.5455/ajvs.57952

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Moreover, our study found that S. aureus is the prominent organism isolated from sheep and goats suffering from mastitis, at 39.28% (11/28 isolates) in sheep and 43.75% (14/32 isolates) in goats. Similar studies supported this finding, i.e., Alemu and Abraha (2017); Abdalhamed et al (2018);and Haggag et al (2019), who revealed that S. aureus is the primary microorganism found in dairy goats because it is detected in ranges from 6% to 40% in all extracted microorganisms.…”

Section: Discussionsupporting

confidence: 64%

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

et al. 2021

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The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the greatest threats to both animal and human health. Our investigation was aimed to identify and differentiate between MRSA and methicillin-sensitive Staphylococcus aureus (MSSA) recovered from mastitic milk using MALDI-TOF mass spectrometry compared with phenotypic methods and studying their susceptibility to various antibiotics. Four hundred milk samples from mastitic animals (cows, sheep, goats, and dromedary camels) were investigated. Phenotypic identification of S. aureus was made through MASTASAPH Latex test, STAPH ID 32, and Vitek 2 system. The proteomic characterization of S. aureus was done by MBT. The Kirby Bauer method was accomplished to detect the resistance of S. aureus strains to antibiotics. The results of the MASTASAPH Latex test, revealed that 54 (46%) were recognized as S. aureus. All S. aureus isolates were identified by MBT with a score of more or equal 2.00. Several peaks were identified in the mass of 4590 Da, 4863 Da, and 4938 Da for MSSA and in the mass of 2636 Da and 3009 Da for MRSA. The MSP dendrogram demonstrated that the S. aureus isolates were classified into one group with a distance level of less or equal 400. The percentage of S. aureus resistance against carbenicillin, erythromycin and kanamycin was 94.4%, 38.88%, and 33.33%, respectively. In conclusion, S. aureus bacteria are among the key triggers for mastitis in Saudi Arabia. MBT is reported to be not only the rapid tool to identify S. aureus but also able to discriminate MRSA from MSSA.

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Section: Discussionsupporting

confidence: 64%

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

et al. 2021

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“…The susceptibility of udder pathogens, including eight CoNS strains of ovine origin, to antibiotics, essential oils, honey, and plant extracts was evaluated [100]. Finally, the role of Staphylococci in subclinical mastitis was examined in 455 sheep milk samples, with 18 isolates being coagulase-negative [101].…”

Section: Egyptmentioning

confidence: 99%

Coagulase-Negative Staphylococci as an Etiologic Agent of Ovine Mastitis, with a Focus on Subclinical Forms

Lysitsas,

Spyrou,

Billinis

et al. 2023

Antibiotics

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The objective of this review is to investigate the distribution and the characteristics of coagulase-negative Staphylococci (CoNS) implicated in ovine mastitis, and especially in subclinical cases, in order to provide a global perspective of the current research data and analyze specific critical aspects of the issue. PRISMA guidelines were implemented in the search of the last 20 years of the related literature in two databases. In total, 139 studies were included in this review. Relevant data were tracked down, assembled, and compared. Regarding the geographical distribution, most studies originated from Europe (68), followed by South America (33). Lacaune was the most examined breed, while S. epidermidis was the predominantly identified species, representing approximately 39% of the obtained isolates. Antibiotic resistance in the relevant bacteria was documented mostly for Penicillin (32.8%) and Amoxicillin (32.1%), while biofilm- and toxin-associated genes were encountered in variable rates because significant inequalities were observed between different articles. Significantly higher rates of antimicrobial resistance were detected in Asia and South America compared to Europe. Finally, the diagnostic procedures carried out in the respective studies were evaluated. Conventional culture and biochemical tests were mostly performed for simple strain identification; therefore, further molecular investigation of isolates should be pursued in future studies, as this will provide important data regarding specific aspects of the implication of CoNS in ovine mastitis.

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Prevalence of Subclinical Mastitis in Small Ruminants and Role of Staphylococcus Species in Such Infection

Cited by 2 publications

References 20 publications

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

Coagulase-Negative Staphylococci as an Etiologic Agent of Ovine Mastitis, with a Focus on Subclinical Forms

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