Prevalence of Staphylococcus aureus and Pasteurella multocida incriminated in calf pneumonia and their treatment

Abstract

B ovine respiratory disease (BRD) is considered as one of the key health issues and is answerable for serious economic losses all over the world. This study aimed to determine a prevalence and proper treatment of Staphylococcus aureus and Pasteurella multocida isolated from calves had respiratory manifestations. Bacteriological examination of 45 nasal swab samples collected from respiratory manifested calves, revealed that 19(42.2%) and 13(28.9%) were S.aureus and P. multocida respectively. S. aureus and P. multocida displayed good sensitivity for gentamicin, ciprofloxacin and chloramphenicol, according to in-vitro antimicrobial susceptibility results.Twenty calves, (3-5 month) were divided into 2 groups. Gr1: contain five of clinically healthy calves kept as a negative control group. Gr2: fifteen calves had respiratory manifestations and positive for S. aureus and P. multocida isolation (kept as positive control group), blood samples for hematological and biochemical studies were taken before treatment. Gr2 (diseased calves) sub divided into three equal groups (Gr3, Gr4 and Gr5) (five calves each). Gr3: were treated with Gentaprima® 1ml /25kg b.wt. I/M for 3 days. Gr4: were treated with Gentaprima® with the same dose+ MENTO-Z ® (1 ml/8 litre drinking water for 5 successive days) and Gr5: were treated with Gentaprima® with the same dose + Levamizole (single S/C injection at dose of 1 mg/50kg bwt).From a bacteriological aspect, all swabs collected in 10 th day after treatment from groups (Gr3, G4 and Gr5) were negative for S.aureus and P. multocida isolation.Hematological study concerning to RBCs count, Hb concentration and PCV% revealed significant decreases in diseased, 3 rd and 10 th days after