Prevalence of specific IgG-antibodies against Toxoplasma gondii in domestic turkeys determined by kinetic ELISA based on recombinant GRA7 and GRA8

Koethe, Martin; Pott, Susan; Ludewig, Martina; Bangoura, Berit; Zöller, Birte; Daugschies, Arwid; Tenter, Astrid M.; Spekker, Katrin; Bittame, Amina; Mercier, Corinne; Fehlhaber, K.; Straubinger, Reinhard K.

doi:10.1016/j.vetpar.2011.03.036

Cited by 26 publications

(20 citation statements)

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“…Comparing the rate of Toxoplasma infection in this study with currently available reports from different geographical areas, higher prevalence of Toxoplasma in turkeys can be seen in this area (10, 11, 22, 23). In Germany, seroprevalence of T. gondii in 1913 turkeys from 14 turkey farms from different areas of the country was 20.2% (ranging from zero to 77.1%) (10). An antibody prevalence of 10% has been reported in turkeys of the southeastern United States (22).…”

Section: Discussionsupporting

confidence: 66%

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Molecular and Serological Evaluation of Toxoplasma gondii Infection in Reared Turkeys in Fars Province, Iran

Sarkari

Asgari

Bagherian

et al. 2014

Jundishapur J Microbiol

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Background:Toxoplasmosis is a parasitic disease caused by the protozoan Toxoplasma gondii. This parasite infects most of warm-blooded animals, including birds. Turkeys are one of these animals which might be infected by this parasite. Little is known about the prevalence of T. gondii in turkeys in Iran.Objectives:The current study aimed to evaluate the rate of Toxoplasma infection in turkeys in Fars Province, Southern Iran.Materials and Methods:Sera and tissues (brain, neck and tongue) of 54 turkeys were collected from Shiraz slaughterhouse in Fars province. Anti-Toxoplasma antibodies were assessed in the collected sera using modified agglutination test (MAT), while tissues were evaluated by polymerase chain reaction (PCR) and bioassay methods.Results:T. gondii antibodies (MAT titer: ≥ 1:40) were found in 89.8% of turkeys. T. gondii DNA was detected in 61.6% of turkey tissues and brain had the highest rate of infection. Brain tissues from each animal were bioassayed and Toxoplasma tissue cysts were found in 11.5% and Toxoplasma DNA in 62% of inoculated mice.Conclusions:Results of this study validated a relatively high level of Toxoplasma infection in reared turkeys and turkey meat might be considered as an infection sources for human.

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Section: Discussionsupporting

confidence: 66%

“…Meat of these birds might be infected by Toxoplasma and transfer the infection to humans. Prevalence of Toxoplasma infection in turkeys varies in different parts of the world, ranging from ≤ 1% to 80% (10-12). …”

Section: Introductionmentioning

confidence: 99%

Molecular and Serological Evaluation of Toxoplasma gondii Infection in Reared Turkeys in Fars Province, Iran

Sarkari

Asgari

Bagherian

et al. 2014

Jundishapur J Microbiol

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“…GRA8 is a conserved gene of T. gondii. Previous studies introduced GRA8 as a marker of acute infection and showed that IgG and IgM ELISA with recombinant GRA8 as antigen was able to differentiate acute from chronic infection [28, 29] and GRA8 ELISA could be used to detect almost all the common strains of T. gondii [30, 31] . In this study, the truncated GRA8 ELISA method showed that the sGRA8 ELISA could detect specific antibody in all of 20 chickens infected with T. gondii 45 DPI, and 95% 60 DPI.…”

Section: Discussionmentioning

confidence: 99%

Detection of Toxoplasma gondii in chicken and soil of chicken farms in Nanjing region, China

Liu

Han

et al. 2017

Infect Dis Poverty

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BackgroundSoil is increasingly recognized as an important source in the transmission of Toxoplasma gondii (T. gondii). The aim of this study was to investigate the presence of T. gondii in the soil and to grasp the relationships between the contamination of soil and chicken infections.MethodsPCR method based on T. gondii-conserved gene internal transcribed spacer 1 (ITS-1) as target gene and ELISA method (sGRA8-ELISA) using the recombinant protein of shortened GRA8 gene of T. gondii as antigen were developed and applied. From April 2013 to March 2014, a total of 700 soil samples were collected at various sites located in thirty farms categorized as free range farm and scale farm in Nanjing, Jiangsu, China, in different seasons. Additionally, a total of 350 sera of chickens were collected from free range farms to determine the presence of antibodies against T. gondii using sGRA8-ELISA.ResultsThe serological results showed that, antibodies were found in 194 of 250 (67.14%) samples from farms with T. gondii positive in soil and 41 of 100 samples from farms with T. gondii negative in soil (41.00%) (P < 0.01). The PCR detection of soil samples showed that, 7 (2.0%) of 350 samples collected from feeding zone in free range farms were found positive of T. gondii, whereas no sample was positive in scale farms. In the seasonal detections, T. gondii was found in 6 (3.33%) samples collected in autumn and 1 (0.56%) collected in winter.ConclusionsThe results indicated that the contamination of T. gondii in soil in the free range farms was higher than that in the scale farms and seroprevalence of T. gondii in chickens in the farm with soil contamination was higher than that with no soil contamination. The soil contamination might be an effective indicator of T. gondii infection in chickens.Electronic supplementary materialThe online version of this article (doi:10.1186/s40249-017-0277-3) contains supplementary material, which is available to authorized users.

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“…In addition, in a study in pregnant women in the urban capital Durango City, consumption of turkey meat was associated with T. gondii infection too [22]. Turkey meat is a potential source for infection with T. gondii [23]. Experimental T. gondii oocyst infections in turkeys have shown the parasite spreading over the whole organism as determined by polymerase chain reaction [24].…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii infection and associated risk factors in Huicholes in Mexico

Alvarado‐Esquivel

Pacheco-Vega

Hernández‐Tinoco³

et al. 2014

Parasites & Vectors

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BackgroundVery little is known about the seroepidemiology of Toxoplasma gondii infection in ethnic groups in Mexico. Huicholes are an indigenous ethnic group living in a remote mountainous region in Mexico. We sought to determine the prevalence of anti-Toxoplasma IgG and IgM antibodies in Huicholes; and to determine the association of Toxoplasma seropositivity with socio-demographic, behavioral, and clinical characteristics of Huicholes.MethodsWe performed a cross sectional survey in Huicholes from September 2013 to January 2014. A convenience sampling method was used. We investigated the prevalence of anti-Toxoplasma IgG and IgM antibodies in 214 Huicholes using enzyme-linked immunoassays. A standardized questionnaire was used to obtain the characteristics of the Huicholes. Bivariate and multivariate analyses were used to assess the association of Toxoplasma exposure and Huicholes’ characteristics.ResultsOf the 214 Huicholes studied (mean age: 37.98 ± 15.80 years), 71 (33.2%) were positive for anti-T. gondii IgG antibodies and 47 (66.2%) of them were also positive for anti-T. gondii IgM antibodies. Seroprevalence of T. gondii infection did not vary with age, sex, or occupation. However, seroprevalence of anti-T. gondii IgM antibodies was significantly higher in female than in male Huicholes. Multivariate analysis of socio-demographic and behavioral characteristics showed that T. gondii exposure was associated with consumption of turkey meat (OR = 2.28; 95% CI: 1.16-4.46; P = 0.01). In addition, seroprevalence of T. gondii infection was significantly higher in Huicholes suffering from dizziness and memory impairment than those without such clinical characteristics.ConclusionsOur results demonstrate serological evidence of T. gondii exposure among Huicholes which may be impacting their health. Results of this first study of T. gondii infection in Huicholes may be useful for the design of optimal preventive measures against infection with T. gondii.

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Prevalence of specific IgG-antibodies against Toxoplasma gondii in domestic turkeys determined by kinetic ELISA based on recombinant GRA7 and GRA8

Cited by 26 publications

References 53 publications

Molecular and Serological Evaluation of Toxoplasma gondii Infection in Reared Turkeys in Fars Province, Iran

Molecular and Serological Evaluation of Toxoplasma gondii Infection in Reared Turkeys in Fars Province, Iran

Detection of Toxoplasma gondii in chicken and soil of chicken farms in Nanjing region, China

Seroprevalence of Toxoplasma gondii infection and associated risk factors in Huicholes in Mexico

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