Prevalence of Shiga toxin-producing Escherichia coli in food products of animal origin as determined by molecular methods

Rantsiou, Kalliopi; Alessandria, Valentina; Cocolin, Luca Simone

doi:10.1016/j.ijfoodmicro.2011.12.010

Cited by 21 publications

(20 citation statements)

References 30 publications

(34 reference statements)

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“…Rantsiou, Alessandria and Cocolin (2012) assessed the genetic diversity of Shiga toxin-producing E. coli from meat and dairy products using ERIC-PCR. Wenz et al (2006) described genetic variability among E. coli isolates from dairy cattle with mastitis using ERIC-PCR.…”

Section: Introductionmentioning

confidence: 99%

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

Oltramari

Cardoso

Patussi

et al. 2014

Braz. J. Pharm. Sci.

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Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance was observed for cephalothin (55.78%). ERIC-PCR revealed high genetic diversity, clustering the 95 bacterial isolates into 90 different genotypic patterns. These results showed a heterogeneous population of E. coli in milk samples produced in the northwestern region of Paraná and the need for good manufacturing practices throughout the processing of pasteurized milk to reduce the risk of foodborne illnesses. Uniterms

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Section: Introductionmentioning

confidence: 99%

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

Oltramari

Cardoso

Patussi

et al. 2014

Braz. J. Pharm. Sci.

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“…The presumptive E. coli isolates in BHI broth were vortexed at 20 Hertz (Velp Scientifica, Usmate, Italy) for approximately 5 s, then 5 µL of each isolate was dispensed onto eosin methylene blue (EMB) agar (CM 69; Oxoid Ltd.) and streaked gently using an inoculation loop. Plates were incubated in an incubator (Infors AG), at 37 o C for 24 h. For molecular confirmation of E. coli, PCR confirmation of the rpoB gene region was performed (11). Suspected isolates were cultured on BHI agar (Lab M, Lancashire, UK) at 37 o C for 24 h, after which a sterile inoculating loop was used to obtain a single colony of each E. coli isolate.…”

Section: Methodsmentioning

confidence: 99%

Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey

Kyere

Bulut

Avsaroglu

et al. 2015

Food Sci Biotechnol

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Some strains of Escherichia coli can be important food borne pathogens. Characterization and antimicrobial resistance testing of 28 E. coli isolates from random food samples obtained in Van, Turkey were performed. Primers for 6 indicator genes (fliC, stx1, stx2, eae, hlyA, and rfbE) for shiga toxin-producing E. coli and 5 indicator genes for each pathogroup (bfpA, aggR, ipaH, daaD, st, and lt) were used. E. coli isolates were also typed using pulsed field gel electrophoresis with the XbaI restriction enzyme. Antimicrobial susceptibility of E. coli isolates was determined using the disk diffusion method for 17 antimicrobials. E. coli isolates were non-pathogenic strains represented by 25 distinguishable PFGE patterns. Antimicrobial susceptibility testing revealed that more than 40% of the E. coli isolates showed resistance to ampicillin, sulphafurazole, and tetracycline. Antimicrobial susceptibility of commensal E. coli should be monitored because these bacteria are becoming reservoirs of antimicrobial resistance genes.

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“…Therefore, development and modifying a general technique of PCR -methods for detection of Shiga toxin-producing strain of E. coli are the focus of attention of scientists in different countries. Often, for rapid detection of the presence of several virulence factors of STEC in the samples, polymerase chain reaction (PCR) in multiplex version is used (Puttalingamma et al, 2012;Rantsiou et al, 2012;Haugum et al, 2014;Hara-Kudo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Shiga toxin-producing strains of Escherichia coli from beef and swine carcasses and the characterization of their genes

Berhilevych

Касянчук

Deriabin

et al. 2018

Regul. Mech. Biosyst.

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Escherichia coli is part of the normal microflora of the intestinal tract of humans and warm-blooded animals, but its presence in raw material and food of animal origin is considered as fecal contamination and can be very dangerous for consumers. The determination of the number of E. coli in raw material and food is important because among them can be pathogenic strains. The most dangerous strains are considered enterohemorrhagic E. coli as a causative agent of severe bloody diarrhea and hemorrhagic uremic syndrome in humans through the production of Shiga-toxin, which is the main virulence factor, responsible for disease. The aim of this study was to identify the prevalence of Shiga toxin-producing strains of E. coli (STEC) from swabs of beef and swine carcass in slaughterhouses in Ukraine and characterize their genes, which are responsible for pathogenic properties. A total of 230 samples of swabs from beef (130) and swine (100) carcasses were obtained from 5 slaughterhouses in Ukraine between 2012 and 2015. Samples of swabs from carcasses were randomly selected at the final point of the process after the final washing of the carcass from the following areas: distal hind limb, abdomen (lateral and medial) from swine carcasses, brisket, flank and flank groin areas from beef carcasses. All samples were examined by culture-dependent method, after that each positive isolate of STEC was analyzed by multiplex PCR to detect the stx1, stx2, and eae genes. Out of 230 collected samples, seven (7.2%) were contaminated with STEC. The highest prevalence of STEC was found in swabs from beef carcasses (8.1%) in comparison to swabs from swine carcasses (5.7%). The stx1 gene was the predominant gene detected in all STEC positive samples. The eae gene was found in one of the examined isolates from beef carcass. Three isolates from swabs of beef carcass carried both stx1 and stx2 genes, one isolate showed association between stx1 and eae genes, one isolate was positive for stx1 gene only. In swabs from swine carcasses (2 isolates) stx1 and stx2 genes were presented simultaneously. The results of this study suggested that fresh raw meat could be a potential vehicle for transmission of the Shiga toxin-producing strain of E. coli to humans. This is the first report of STEC prevalence in beef and swine carcasses in Ukraine and these data will be valuable for microbiological risk assessment and help the appropriate services to develop strategies to mitigate health risk.

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Prevalence of Shiga toxin-producing Escherichia coli in food products of animal origin as determined by molecular methods

Cited by 21 publications

References 30 publications

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey

Isolation of Shiga toxin-producing strains of Escherichia coli from beef and swine carcasses and the characterization of their genes

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