Prevalence of Shiga toxin-producing Escherichia coli (STEC) O157:H7, Six non-O157 STECs, and Salmonella on beef carcasses in Provincially Licensed Abattoirs in Alberta, Canada

Essendoubi, Saida; Stashko, Natisha; So, Iyla; Gensler, Gary; Rolheiser, Deana; Mainali, Chunu

doi:10.1016/j.foodcont.2019.05.032

Cited by 16 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ruminants, particularly cattle, are reservoirs and asymptomatic carriers of Salmonella (Cummings et al 2010;Gutema et al 2019) and E. coli O157 (Gyles 2007). Studies reported the occurrence of these pathogens in the feces and on the hides of cattle on farms and in slaughterhouses in developed countries (Arthur et al 2010;Cobbaut et al 2008;Essendoubi et al 2019;Madoroba et al 2016). The presence of Salmonella and E. coli O157 in the feces and on the hides of cattle may lead to their transfer to carcasses during hide removal and evisceration (Croxen et al 2013;Cummings et al 2010;Gutema et al 2021a).…”

Section: Introductionmentioning

confidence: 99%

Assessment of beef carcass contamination with Salmonella and E. coli O 157 in slaughterhouses in Bishoftu, Ethiopia

et al. 2021

View full text Add to dashboard Cite

Background Salmonella and E. coli O157 are common causes of foodborne diseases. Evisceration and de-hiding steps can lead to carcass contamination during slaughter operation. In Ethiopia, information on the association between the presence of these pathogens in the rectal content and/or on the hide of cattle and their presence on the carcass is lacking. Methods The aim of this study was to assess the sources of beef carcass contamination with Salmonella and E. coli O157 during slaughter. Rectal contents and hide- and carcass-swabs (from three sites: foreleg, brisket and hind leg) were collected from 70 beef cattle at two small scale slaughterhouses. Isolates were genotyped by the Pulsed Field Gel Electrophoresis method and tested for resistance against 14 microbial drugs. Results Salmonella was detected at equal proportions (7.1%) in rectal content samples and hide swabs. E. coli O157 was detected in 8.6% of the rectal contents and 4.3% of the hide swabs. The proportion of contaminated carcasses was 8.6% for Salmonella and 7.1% for E. coli O157. Genetic linkage between the Salmonella and E. coli O157 isolates from the rectal contents and/or hides and carcasses were observed only in a few cases (2 and 1 carcasses, respectively) indicating the limited direct transfer of the pathogens from the feces and/or hide to the carcass during slaughter. Most carcasses became positive by cross contamination. All the S. Typhimurium isolates (n = 8) were multidrug resistant being resistant to ampicillin, chloramphenicol, sulfamethoxazole and tetracycline. The two S. Dublin isolates were resistant to colistin. All E. coli O157 isolates were susceptible to the antimicrobials tested. Conclusion The results indicated that cross contamination may be an important source for carcass contamination.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of beef carcass contamination with Salmonella and E. coli O 157 in slaughterhouses in Bishoftu, Ethiopia

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This important step is usually a Critical Control Point (CCP) within a risk-based food safety management system (Hazard Analysis and Critical Control Points/HACCP) and reduces both, the bacterial numbers and prevalence of pathogens (22). APC bacteria are generally used to assess the hygiene of meat processing (24) and EBC are also used as indicators of fecal contamination (25,26). The results of the present study showed that scalding is effective in reducing bacterial contamination on the carcass.…”

Section: Discussionmentioning

confidence: 78%

“…Only during winter was significantly lower prevalence (P ˂ 0.05) of this pathogen indicator (stx) found compared to other seasons. Essendoubi et al(26) also found higher prevalence of STEC on beef carcasses during warmer months (from June to November), while Dawson et al(39) reported higher E. coli O157:H7 colonization in cattle during warmer months compared to cooler times of the year in various cattle production systems. One possible explanation may be that animals are dirtier during summer months due to soil and fecal contamination(33,40,41).…”

mentioning

confidence: 97%

Seasonal prevalence and characterization of Shiga toxin-producing Escherichia coli on pork carcasses at three steps of the harvest process at two commercial processing plants in the US

Nastasijević¹,

Schmidt

Bošković

et al. 2020

Preprint

View full text Add to dashboard Cite

Shiga toxin (stx) -producing Escherichia coli (STEC) are foodborne pathogens that have a significant impact on public health, with those possessing the attachment factor intimin (eae) referred to as enterohemorrhagic E. coli (EHEC) associated with life threatening illnesses. Cattle and beef are considered typical sources of STEC, but their presence in pork products is a growing concern. Therefore, carcasses (n=1536) at two U.S. pork processors were sampled once per season at three stages of harvest (post-stunning skins; post-scald carcasses; chilled carcasses) then examined using PCR for stx and eae, aerobic plate count (APC) and Enterobacteriaceae counts (EBC). Skins, post-scald, and chilled carcasses had prevalence of stx (85.3, 17.5, and 5.4%, respectively), with 82.3, 7.8, and 1.7% respectively, having stx and eae present. All stx positive samples were subjected to culture isolation that resulted in 368 STEC and 46 EHEC isolates. The most frequently identified STEC were serogroup O121, O8, and O91(63, 6.7, and 6.0% of total STEC, respectively). The most frequently isolated EHEC was serotype O157:H7 (63% of total EHEC). Results showed that scalding significantly reduced (P < 0.05) carcass APC and EBC by 3.00 and 2.50 log10 CFU/100 cm2 respectively. A seasonal effect was observed with STEC prevalence lower (P < 0.05) in winter. The data from this study shows significant (P < 0.05) reduction in the incidence of STEC (stx) from 85.3% to 5.4% and of EHEC (stx+eae) from 82.3% to 1.7% within slaughter-to-chilling continuum, respectively, and that potential EHEC can be confirmed present throughout using culture isolation.IMPORTANCESeven serogroups of Shiga toxin-producing Escherichia coli (STEC) are responsible for most (>75%) cases of severe illnesses caused by STEC and are considered adulterants of beef. However, some STEC outbreaks have been attributed to pork products although the same E. coli are not considered adulterants in pork because little is known of their prevalence along the pork chain. The significance of the work presented here is that it identifies disease causing STEC, enterohemorrhagic E. coli (EHEC), demonstrating that these same organisms are a food safety hazard in pork as well as beef. The results show that most STEC isolated from pork are not likely to cause severe disease in humans and that processes used in pork harvest, such as scalding, offer a significant control point to reduce contamination. The results will assist the pork processing industry and regulatory agencies to optimize interventions to improve the safety of pork products.

show abstract

“…Cassette PCR is inexpensive and ready to use. We reported testing of 820 beef carcass swabs for the presence of presumptively pathogenic E. coli by cassette PCR, as compared to conventional liquid PCR [ 13 ] for carcass swabs that had been collected over a one-year period from provincially inspected processing plants in Alberta by Alberta Agriculture and Forestry (AAF) [ 18 ]. The same samples were also tested by AAF using BAX PCR.…”

Section: Introductionmentioning

confidence: 99%

Comparison of a Miniaturized Cassette PCR System with a Commercially Available Platform for Detecting Escherichia coli in Beef Carcass Swabs

et al. 2021

View full text Add to dashboard Cite

Detection sensitivity of cassette PCR was compared with a commercial BAX® PCR system for detection of eae and stx genes in Escherichia coli from 806 beef carcass swabs. Cassette PCR detects multiple genetic markers on multiple samples using PCR and melt curve analysis. Conventional PCR served as a gold standard. Overall, for positive and negative concordance, cassette PCR was 98.6% concordant with conventional PCR, and BAX PCR was 65.4% concordant. Of 806 beef carcass swabs, 339 by cassette PCR and 84 by BAX PCR harbored eae + stx+E. coli. For BAX PCR reactions, 84% of eae+ swabs, 79% of stx+ swabs, and 86% of eae + stx+ swabs were also detected by cassette PCR. For cassette PCR reactions, 457 swabs were eae+ with only 117 scored as eae+ using BAX PCR for 26% positive concordance. For stx primers, cassette PCR scored 480 samples as stx+ but only 215 samples were stx+ by BAX PCR, giving 45% positive concordance. Importantly, cassette PCR scored 339 swabs as harboring eae + stx+ E. coli, but BAX PCR detected only 71 positives giving only 21% positive concordance, with many false negatives. Cassette PCR is a highly sensitive method for detection of STEC genes in E. coli found in carcass swabs.

show abstract

Prevalence of Shiga toxin-producing Escherichia coli (STEC) O157:H7, Six non-O157 STECs, and Salmonella on beef carcasses in Provincially Licensed Abattoirs in Alberta, Canada

Cited by 16 publications

References 29 publications

Assessment of beef carcass contamination with Salmonella and E. coli O 157 in slaughterhouses in Bishoftu, Ethiopia

Assessment of beef carcass contamination with Salmonella and E. coli O 157 in slaughterhouses in Bishoftu, Ethiopia

Seasonal prevalence and characterization of Shiga toxin-producing Escherichia coli on pork carcasses at three steps of the harvest process at two commercial processing plants in the US

Comparison of a Miniaturized Cassette PCR System with a Commercially Available Platform for Detecting Escherichia coli in Beef Carcass Swabs

Contact Info

Product

Resources

About