Prevalence of Sarcocystis in slaughtered one-humped camels (Camelus dromedarius) in Iran

Shekarforoush, Seyed Shahram; Shakerian, Amir; Hasanpoor, M. M.

doi:10.1007/s11250-006-4362-z

Cited by 16 publications

(24 citation statements)

References 10 publications

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“…Lack of relationship between sex and infection rates has shown in similar studies on camels before (Woldemeskel and Gumi 2001;Shekarforoush et al 2006;Valinezhad et al 2008). In the present study, infection rates' risk in higher aged camels was observed.…”

Section: Resultssupporting

confidence: 53%

“…Different researches on camelids' sarcocystosis have reported dissimilar tissue patterns. Higher incidence in esophagus was reported previously by Woldemeskel and Gumi (2001) and Valinezhad et al (2008), while Shekarforoush et al (2006) found heart as the most infected organ. Fatani et al (1996) reported diaphragm of camels to be the most common site.…”

Section: Resultssupporting

confidence: 40%

“…Similarly high prevalence rates were reported in neighboring countries; Iraq (91.6 %; Latif et al 1999), Afghanistan (47.3-66.3 %;Kirmse and Mohanbabu 1986), United Arab Emirates (50 %; El-Afifi et al 1963) and Saudi Arabia (88.4 %; Fatani et al 1996). Former studies on Sarcocystis prevalence in other regions of Iran revealed 52.6, 52.3 and 83.6 % in Tehran, Esfahan and Mashhad, respectively (Rahbari et al 1981;Shekarforoush et al 2006;Valinezhad et al 2008). Differences between the previously reported infection rates may be due to different husbandry management systems in northern and eastern parts of Iran, as well as diagnostic methods.…”

Section: Resultsmentioning

confidence: 99%

“…Dogs can be infected with S. cameli after ingestion of camel meat, so are important in spreading the infection (Hilali et al 1995). Several authors from Iran have detected sarcocystosis in camels from different parts of the country by various diagnostic methods, and reported its prevalence to be up to 86.3 % (Rahbari et al 1981;Shekarforoush et al 2006;Valinezhad et al 2008;Motamedi et al 2011). Therefore, the aim of this study was to determine distribution patterns of camel Sarcocystis in different organs, as well as the influence of age on infection rate in Yazd province.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and distribution patterns of Sarcocystis in camels (Camelus dromedarius) in Yazd province, Iran

et al. 2012

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Sarcocystis spp., are zoonotic cyst-forming coccidian parasites that cause sarcocystosis. This study was conducted to investigate the prevalence as well as distribution patterns of Sarcocystis spp. infection in slaughtered one-humped camels of Yazd province, Iran. Muscles of 130 camels were investigated for either macroscopic or microscopic sarcocysts during summer 2009. No macroscopic cyst was observed in the animals at naked eye inspection. Of examined camels, 67 (51.5 %) were positive for bradyzoites of the parasite by pepsin-digestion method. The infection rates of infected animals were 55.22, 50.75, 38.81, 34.33, and 28.36 %, in esophagus, heart, masseter muscle, intercostal muscle and limb muscle, respectively. Esophagus was the most commonly infected organ. No significant difference in the rate of infection between male (52.08 %) and female (51.22 %) camels was observed. Logistic regression analyses showed that infection rates' risk increased with increment in age of camels. This considerable prevalence of microscopic Sarcocystis spp. in Yazd province camels reflects a significant role played by wild and domestic carnivores in the transmission of these parasites.

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Section: Resultssupporting

confidence: 53%

Section: Resultssupporting

confidence: 40%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and distribution patterns of Sarcocystis in camels (Camelus dromedarius) in Yazd province, Iran

et al. 2012

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“…(Hamidinejat et al, 2013). Other studies in different provinces of Iran showed that 97% of sheep had Sarcocystis infection (Shekarforoush et al, 2006). In previous studies throughout the world, species of Sacocystis were isolated from different animals (daSilva et al, 2009;Al-Hoot et al, 2009 The macrosarcocysts species are almost nonpathogenic but are responsible for economic losses because of the complete or partial rejection of the animal carcasses at slaughterhouses (Oryan et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of Microscopic Cysts of Sarcocystis in Sheep in Baghdad Province

ali¹,

Hasanhatthot²,

alilateef³

2016

Int. J. Adv. Res. Biol. Sci

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Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. This study was aimed to identify the macroscopic and microscopic cysts of Sarcocystis in sheep by using PCR. One hundred random samples taken from sheep at abattoir. The digestion method was used for bradyzoites observation in, esophagus and inter costal muscle samples. PCR analysis was conducted on microscopic cysts and the other samples. Sequencing was performed for ten PCR products. Genotypes were identified by BLAST search and homology analysis. Digestion method and PCR analysis revealed positive results in all samples taken from esophagus and inter costal muscle.

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Ultrastructural and molecular characterization of Sarcocystis isolated from camel (Camelus dromedarius) in Iran

et al. 2010

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Sarcocystis cameli was first described in one-humped camels (Camelus dromedarius), and it is the only species which have so far reported in camels. Although more than 150 species of Sarcocystis were described in various animals, only a few data on camel Sarcocystis ultrastructure were published, and this report is the first for molecular information (DNA sequence and RLFP digestion pattern). The main objective of the present work is to characterize Sarcocystis isolated from camels by electron microscopy and PCR-RFLP methods. Muscle samples were taken from the fresh esophagus, diaphragm, skeletal muscles, and heart of one-humped camels (C. dromedarius) slaughtered in abattoirs of Tehran and Ghazvin provinces, Iran. The dissection and trypsin digestion techniques were applied for the detection of the cysts. The infected samples were fixed in glutaraldehyde and/or frozen at -20°C until use for ultrastructural and molecular studies, respectively. The ultrastructural and molecular studies were carried out contemporaneously. The 18S rRNA gene of the parasites was amplified by PCR. The PCR products were cloned into a pTZ57R/T and sequenced. In addition, the PCR products were digested separately with each of the four restriction enzymes for RFLP. Our results indicated that only microcysts were observed in muscle samples. The microcysts were white, elongated, spindled, and a few spiral-shaped, with mean size 260 × 75 μm which are identical with S. cameli. The ultrastructure of microcyst wall had many non-branched finger-like protrusions irregularly folded. There was a 600-bp specific band amplified after PCR with specific primers. The molecular data for camel Sarcocystis is reported for the first time in Iran and the world.

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Prevalence of Sarcocystis in slaughtered one-humped camels (Camelus dromedarius) in Iran

Cited by 16 publications

References 10 publications

Prevalence and distribution patterns of Sarcocystis in camels (Camelus dromedarius) in Yazd province, Iran

Prevalence and distribution patterns of Sarcocystis in camels (Camelus dromedarius) in Yazd province, Iran

Molecular identification of Microscopic Cysts of Sarcocystis in Sheep in Baghdad Province

Ultrastructural and molecular characterization of Sarcocystis isolated from camel (Camelus dromedarius) in Iran

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