Prevalence of Salmonella enteritidis in Spent Hens

Waltman, W. D.; Horne, April; Pirkle, Carl I.; Johnson, D C

doi:10.2307/1591498

Cited by 23 publications

(7 citation statements)

References 4 publications

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“…Although prevalence of Salmonella in pooled cecal contents in spent hens has been reported [29], none has been reported on the prevalence of Salmonella in cecal contents of chickens in Japan. Since Salmonella detection of 14.3% in cecal contents in the present study was lower than 65.4% of cecal pools from spent laying hens reported in the U.S.A. [29], 25% of cloacal swab collected from broilers in Thailand [26], and 60 to 80% of poultry carcasses examined in Spain [7], actual infection rate of Japanese chickens with Salmonella might be lower than those of foreign countries. This lower infection rate might be due to the difference in incubation temperature, types of enrichment media and selecting plating media used for isolation of Salmonella in our study.…”

Section: Discussionmentioning

confidence: 98%

Prevalence and Persistence of Salmonella in Broiler Chicken Flocks.

et al. 1999

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ABSTRACT. Cecal contents of 2,345 broiler chickens consisting of 28 flocks originated from 12 farms were examined for the prevalence of Salmonella to know the actual status of infection with Salmonella in the chicken flocks. Salmonella was isolated from 336 (14.3%) samples. From these isolates, eight serovars were identified. Of the 336 Salmonella isolates, 242 (72.0%) were serotyped as S. Blockley, 60 (17.9%) S. Hadar, 15 (4.5%) S. Bredeney, nine (2.7%) S. Schwarzengrund, four (1.2%) S. Anatum, three (0.9%) S. Enteritidis, two (0.6%) S. Ohio, and one (0.3%) S. Livingstone. The same serovars of Salmonella were repeatedly found in the chickens from the same farms. S. Typhimurium and S. Enteritidis were detected in pooled broken eggshell samples collected from the hatchery. Analysis of plasmid profiles revealed 11 patterns of S. Blockley and seven patterns of S. Hadar. Strains of the same plasmid profiles of S. Blockley were isolated repeatedly from the same farm over one year after the first isolation.-KEY WORDS: broiler, hatchery, poultry farm, prevalence, Salmonella.J. Vet. Med. Sci. 61(3): 255-259, 1999 cooled in an icebox and immediately transported to our laboratory. Isolation and identification of Salmonella : To each bag of cecal contents, added was 9 volumes of selenite cystine broth (SCB, Nissui). These samples were incubated at 37°C for 48 hr, then each sample was inoculated onto a brilliant green agar (BBL) plate supplemented with 20 µg novobiocin/ml (BGN) and a desoxycholate hydrogen sulfide lactose (DHL) agar (Nissui) plate. The plates were incubated at 37°C for 24 hr.

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Section: Discussionmentioning

confidence: 98%

Prevalence and Persistence of Salmonella in Broiler Chicken Flocks.

et al. 1999

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“…characters of the isolated Salmonellae (Waltman et al, 1998) The cultural characters of the isolated Salmonellae appeared on XLD agar as smooth pink colonies with black center (H2S production) while H-E agar it appeared as deep blue colored colonies. On MacConkey's agar the colonies were pale, colorless smooth, transparent (non lactose fermenter), however, on S-S agar it appeared as pale colored colonies with or without black centers.…”

Section: Cultural Staining and Biochemicalmentioning

confidence: 99%

Studies on different salmonella serotypes isolated from poultry in different governorates in Egypt

Tawab

Hofy

Ammar

et al. 2015

Benha Veterinary Medical Journal

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“…In order to assess the ability of the assay to detect serovar Enteritidis among a mixed population of bacteria, six species were selected. S. enterica serovar Heidelberg has been frequently isolated from poultry and egg shells (3,16,23,29), while Escherichia coli, Proteus vulgaris, Citrobacter freundii (ATCC 8090), Alcanigenes faecalis, and Pseudomonas fluorescens are common spoilage organisms associated with eggs (5). Samples were inoculated with 33, 50, 67, 100, or 133 CFU of each bacterial species per ml.…”

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confidence: 99%

Application of Rapid Dot Blot Immunoassay for Detection of Salmonella enterica Serovar Enteritidis in Eggs, Poultry, and Other Foods

Yoshimasu

Zawistowski

2001

Appl Environ Microbiol

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Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre-or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.Conventional methods for detection of Salmonella in foods are labor-intensive, time-consuming, and expensive. It has also been found that some of the routinely used selective enrichment broths are inhibitory towards Salmonella enterica serovar Enteritidis (28). Rapid methods based on principles such as membrane technology (11), latex agglutination (27), immunoassays (6,18,30), and immunomagnetic separation (8,9,19) have been developed. Methods employing PCR in combination with preenrichment broths (24,31,32), immunomagnetic separation (25, 26), or centrifugation (21, 22) are currently being developed.Immunologically based methods specific for serovar Enteritidis suffer from the same drawbacks as the above methods: one or more enrichment broths, or in some cases, postenrichment broths, are required. Cross-reactivity has also been observed with most monoclonal antibodies produced against serovar Enteritidis (17,18,27). This report describes the development of a rapid dot blot immunoassay for the detection of serovar Enteritidis in eggs, poultry, and other products.Large grade A eggs were scrubbed with 70% ethanol and opened aseptically. Eggs were mixed for 30 s using a stomacher lab blender 400 (Seward Laboratory, London, England) and were inoculated with either serovar Enteritidis phage type 1, 4, 8, 13, or 13a. After incubation, 25-ml portions were placed into 50-ml polypropylene tubes, and 0.1 volume of 15% sodium cholate in phosphate-buffered saline (PBS) (pH 7.2) was added. After being mixed, tubes were placed in boiling water for 10 min and cooled for 30 min at 4°C. Through heating, the egg mixture was solidified, forming a solid egg gel. After cooling, the gel was removed from the tube, and a sterile core borer (10-mm diameter) was used to create small cylindrical gels. The cylindrical gels were then cut into disks of 2 mm in thickness.Nitrocellulose strips (8.5 by 2.5 cm) were prewetted with PBS prior to use. Egg disks were placed on the membrane for 5 min, removed, and washed for 2 min in PBS. Strips were blocked for 45 min in 5% skim milk powder in Tris-buffered saline (pH 7.5) and incubated with a murine monoclonal antibody solution (tissue culture supernatant) for 45 min, followed by 1 h of incubation in biotinylated goat anti-mouse immunoglobulin G. Strips were incubated with streptavidinalkaline phosphatase for 1 h and developed with a BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium chloride substrate solution. Membranes were washed twice with Tris-buffered saline containing 0.05% Tween 20 for 2 min between each step. All steps were performed at room temperature.Bacterial cultures were...

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Prevalence of Salmonella enteritidis in Spent Hens

Cited by 23 publications

References 4 publications

Prevalence and Persistence of Salmonella in Broiler Chicken Flocks.

Prevalence and Persistence of Salmonella in Broiler Chicken Flocks.

Studies on different salmonella serotypes isolated from poultry in different governorates in Egypt

Application of Rapid Dot Blot Immunoassay for Detection of Salmonella enterica Serovar Enteritidis in Eggs, Poultry, and Other Foods

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