Prevalence of Rotavirus in shellfish from Southern Kerala

Mohan, Vysakh; Rawat, Shashi; Lokesh, K.; Mohan, Hari; Reddy, D. Avinash; Kumar, Ashok; Bhilegaonkar, K.N.

doi:10.14202/vetworld.2014.821-824

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…These findings are consistent with those of a previous study conducted by different authors that evaluated the use of mussels in biomonitoring marine environments. We proved the presence of rotavirus in Mytilus galloprovincialis and found the viral contamination rate of the mussel samples to be relatively high (37.5%), even though the observed rate of prevalence was not as high as that reported by a follow-up of mussels in France (21). Congruent results have been reported by a variety of similar studies (16,27).…”

Section: Discussionsupporting

confidence: 53%

“…Reverse transcription polymerase chain reaction (RT-PCR) was used to detect enteric viruses in the Mytilus galloprovincialis mussel samples ( 7 ), because it is a successful method for detecting viruses in shellfish ( 21 ). The mussels, collected from each sampling location in spring and autumn of each year, had lengths ranging between 50 and 80 mm in spring and 40–70 mm in autumn.…”

Section: Methodsmentioning

confidence: 99%

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Possibilities of using mussels (Mytilus galloprovincialis) to predict rotavirus contamination in Albania

Lugaj,

Korro,

Tolomeo

et al. 2023

Journal of Veterinary Research

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Introduction Rotaviruses are non-enveloped viruses that each consist of 11 double-stranded RNA molecules. These viruses are able to persist in the environment, and therefore play a fundamental role in the epidemiology of gastroenteritis and severe diarrhoea in children worldwide. While mussels have been primarily used as indicators of chemical pollution, they can also be used to monitor viral contamination. The purpose of this study was to demonstrate that the Mytilus galloprovincialis mussel can also be used to detect microbial contamination, owing to its tendency to naturally concentrate viruses and other pathogens. Material and Methods A total of 102 Mytilus galloprovincialis mussel samples from Albania were collected over a three-year period: 37 samples off the Cape of Stillo in 2015, 39 samples from Butrinti Lake in 2019 and 26 samples from Butrinti Lake in 2021. Results The presence of rotavirus in the Cape of Stillo samples in 2015 was noted in 47% of samples from site 1, 33% from site 2, and 52% from site 3. In Butrinti Lake the percentage of infected individuals in 2019 was 33% from site 1, 41% from site 2, and 33% from site 3, whereas in 2021, it was 50% from site 1, 19% from site 2, and 0% from site 3. In total the percentage of infected individuals off the Cape of Stillo in 2015 was 44%, in Butrinti Lake in 2019 it was 36%, and in Butrinti Lake in 2021 it was 23 %. Conclusion These results indicate the presence of rotavirus in the shellfish specimens tested, and further analysis is needed to assess the potential health risks associated with consuming these shellfish. This study also indicates that mussels can be used in marine virological surveillance programmes.

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Section: Discussionsupporting

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Possibilities of using mussels (Mytilus galloprovincialis) to predict rotavirus contamination in Albania

Lugaj,

Korro,

Tolomeo

et al. 2023

Journal of Veterinary Research

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“…The total incidence of RoV amongst shellfish samples was 0.66%. Italy (0.50%) (24) contributed to a lower incidence rate of RoV in shellfish samples, whereas India (2.50%) (25) and France (27%) (26) formed higher incidence rates. The findings of the present survey revealed a higher incidence of NoV GII amongst shellfish samples.…”

Section: Discussionmentioning

confidence: 99%

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf

2023

ARI

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Shellfishes are significant economic and nutritious seafood amongst people of different countries. Seafood products, particularly shellfish samples, are potential reservoirs of enteric viruses. This research investigated the incidence of RoV, NoV GI and GII, HAV, and HEV in shellfish samples from the Persian Gulf, Iran. One hundred and fifty shellfish samples were collected. RNA extraction and cDNA synthesis was performed using commercial kits. The real-time PCR assessed the presence of enteric viruses in extracted cDNA samples. Thirty-two out of 150 (21.33%) shellfish samples were contaminated with enteric viruses. Prevalence of NoV GI, NoV GII, HAV, and RoV amongst shellfish samples were 8.00%, 11.33%, 1.33%, and 0.66%, respectively. There were no contaminated shellfish samples with HEV. Simultaneous prevalence of HAV and NoV GI and HAV and NoV GII viruses were 0.66% and 0.66%, respectively. Examined viruses had a higher prevalence in shellfish samples collected through the winter season (P < 0.05). Prevalence of HAV, RoV, NoV GI, and NoV GII amongst shellfish samples collected through the winter season was 2.85%, 9.09%, 11.90%, and 20%, respectively. To the best of our knowledge, this is the first report of the incidence of enteric viruses, particularly HAV, NoV GI, NoV GII, and RoV, in shellfish samples from the Persian Gulf, Iran. Shellfish samples may serve as a potential source of enteric viruses for the human population. Therefore, routine viral assessments should be conducted. Completely cooking shellfish samples before consumption can significantly reduce the risk of HAV, RoV, NoV GI, and NoV GII infections. Furthermore, given the export value and importance of shellfish samples, their microbial quality and safety should be routinely monitored.

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“…The high prevalence of EV in bivalves such as clams and oysters can be attributed to their filter feeding habit which enables them to accumulate enteric viruses from the surrounding water [ 13 ]. The presence of enteric viruses in bivalves collected from fish landing centers and retail supermarkets indicates that they are marketed without adequate depuration process to remove these pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…In general, enteric viral infections are mild and self-limiting. However, infections can occasionally lead to complications involving hepatitis, conjunctivitis, allergies, encephalitis, myocarditis, pericarditis, and foot and mouth disease [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of human enterovirus in tropical fish and shellfish and their relationship with fecal indicator bacteria

et al. 2018

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Aim:Human enteroviruses in fish and shellfish are a health concern worldwide. Human infections occur due to the consumption of raw or insufficiently cooked fish or shellfish. The objective of this study was to determine the occurrence of human enteric viruses belonging to Enterovirus (EV) group in seafood in Mumbai and to correlate their occurrence with the bacterial indicators of fecal contamination.Materials and Methods:Samples of fresh fish and shellfish collected from fish landing centers and retail fish markets were analyzed by virus concentration, nucleic acid extraction, and reverse transcription-polymerase chain reaction (RT-PCR). Bacterial indicators of fecal contamination were estimated by the most probable number technique. The relationship between the presence of virus and fecal indicators was determined by statistical analysis.Results:A total of 89 samples comprising of fish, shrimps, oysters, clams, and mussels were screened in this study. EV was detected in 32 (35.95%) samples, and all the virus-positive samples belonged to bivalve molluscan group. None of the finfish and crustacean shellfish samples was positive for the enteric viruses. Clams were found to be the most contaminated with 48.4% of the samples being positive for EV. The prevalence of enteric viruses in seafood samples showed a strong positive correlation with the bacteriological indicators of fecal contamination, suggesting that fecal coliform bacteria are good indicators of EVs in tropical seafood.Conclusion:The presence of EVs in seafood is a public health hazard. Increasing level of coastal water contamination from anthropogenic sources is the primary reason for the contamination of seafood with EVs. Continuous monitoring of coastal waters and seafood for enteric viruses will help to ensure the safety of fish and shellfish for human consumption.

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Prevalence of Rotavirus in shellfish from Southern Kerala

Cited by 7 publications

References 18 publications

Possibilities of using mussels (Mytilus galloprovincialis) to predict rotavirus contamination in Albania

Possibilities of using mussels (Mytilus galloprovincialis) to predict rotavirus contamination in Albania

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf

Occurrence of human enterovirus in tropical fish and shellfish and their relationship with fecal indicator bacteria

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