Prevalence of Root Canal Treatment in First Molars among Children in Chennai - An Institutional Retrospective Study

Raj, SS

doi:10.19070/2377-8075-si02-07002

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“…Odontogenic tumors and cysts are a heterogeneous group of diseases derived from the cells of the tooth forming apparatus and their remnants [1] [2]. Ameloblastoma (AM) is among the most common benign odontogenic tumors and is clinically characterized by features such as slow infiltrative growth mainly involving the mandible, an asymptomatic presentation, and high recurrence rate when treated with conservative surgery [2] [3].…”

Section: Introductionmentioning

confidence: 99%

RASSF1A Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

Udompatanakorn¹,

Yada²,

Matsuo³

2020

OJPathology

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Background: The etiology and pathogenesis of odontogenic lesions remain to be determined. Previous studies have identified epigenetic and genetic alterations that may be relevant to lesions progression and development. Hypermethylation of the Ras association domain family protein 1A (RASSF1A) has been observed in a variety of human cancers. However, the methylation status of RASSF1A in odontogenic lesions remains unknown. Thus, the aim of this study was to investigate the prevalence of RASSFA promoter hypermethylation and v-raf murine sarcoma viral oncogene homolog B V600E mutant (BRAF V600E) expression as well as the correlations between these alterations and clinicopathological features of patients with odontogenic lesions. Methods: We subjected 66 formalin-fixed, paraffin-embedded odontogenic lesions [ameloblastoma (AM), 21; ameloblastic carcinoma (AC), 6; odontogenic keratocyst (OKC), 19; and dentigerous cyst (DC), 20] to methylation-specific polymerase chain reaction to determine RASSF1A hypermethylation and immunohistochemistry to detect BRAF V600E protein expression. Results: We observed RASSF1A hypermethylation in 20% (4/20; methylation could not be detected in one lesion), 100% (6/6), 26.3% (5/19), and 5% (1/20) of AM, AC, OKC, and DC samples, respectively. RASSF1A methylation was significantly more frequently observed in AC relative to AM, OKC, and DC (p < 0.001). Moreover, 85.7% (18/21) and 83.3% (5/6) AM and AC samples, respectively, were BRAF V600E-positive, whereas all OKC and DC sample were BRAF V600E-negative. No correlations of RASSF1A methylation and BRAF V600E expression with clinicopathological features were observed. Conclusions: Concomitant RASSF1A methylation and positive BRAF V600E expression are commonly observed in AC, which may contribute to AC tumorigenesis.

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Section: Introductionmentioning

confidence: 99%

RASSF1A Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

Udompatanakorn¹,

Yada²,

Matsuo³

2020

OJPathology

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Prevalence of Root Canal Treatment in First Molars among Children in Chennai - An Institutional Retrospective Study

Cited by 1 publication

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<i>RASSF</i>1<i>A</i> Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

<i>RASSF</i>1<i>A</i> Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

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Prevalence of Root Canal Treatment in First Molars among Children in Chennai - An Institutional Retrospective Study

Cited by 1 publication

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&lt;i&gt;RASSF&lt;/i&gt;1&lt;i&gt;A&lt;/i&gt; Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

&lt;i&gt;RASSF&lt;/i&gt;1&lt;i&gt;A&lt;/i&gt; Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

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<i>RASSF</i>1<i>A</i> Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

<i>RASSF</i>1<i>A</i> Methylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions