Prevalence of resistance-associated mutations in Human Immunodeficiency Virus type 1-positive individuals failing HAART in Rio de Janeiro, Brazil

Varella, Rafael Brandão; Ferreira, Simone Bacellar Leal; Castro, Márcia Braga de; Tavares, Marisa Dias; Zalis, Mariano

doi:10.1590/s1413-86702008000500007

Cited by 6 publications

(2 citation statements)

References 35 publications

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“…The frequency of TAMs found for NNRTI and PI resistance mutations in the co-infected patients was lower than the frequency reported in the literature for mono-infected patients (Table 2 ) 27 , 28 . It has been shown that co-infection with parasites can alter the HIV dynamic 23 .…”

Section: Discussioncontrasting

confidence: 73%

HIV Reverse Transcriptase and Protease Genes Variability Can Be a Biomarker Associated with HIV and Hepatitis B or C Coinfection

Cantão

Almeida

Wolf

et al. 2018

Sci Rep

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Variability of the HIV reverse transcriptase (RT) and protease (PR) genes has been used as indicators of drug resistance and as a mean to evaluate phylogenetic relationships among circulating virus. However, these studies have been carried in HIV mono-infected populations. The goal of this study was to evaluate, for the first time, the HIV PR and RT sequences from HIV/HBV and HIV/HCV co-infected patients. HIV PR and RT genes were amplificated and sequenced to resistance analysis. The bioinformatics analysis was performed to infer about sequences clustering and molecular evolution. The results showed that the most frequent amino acid substitutions in RT were L214F (67.6%), I135T (55.9%), and in PR was V15I (41.2%). The molecular clock analysis showed that the HIV circulating in co-infected patients were separated in two clusters in the years 1999–2000. Some patients included as HIV mono-infected according patients’ medical records and inside the co-infected cluster were, in fact, co-infected by PCR analysis. Analysis of the decision trees showed susceptibility to lamivudine and emtricitabine were important attribute to characterize co-infected patients. In conclusion, the results obtained in this study suggest, for the first time, that HIV RT and PR genes variability could be a genetic biomarker to coinfection.

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Section: Discussioncontrasting

confidence: 73%

HIV Reverse Transcriptase and Protease Genes Variability Can Be a Biomarker Associated with HIV and Hepatitis B or C Coinfection

Cantão

Almeida

Wolf

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Several previous studies have shown a similar level of drug resistance in different Brazilian regions; however the sample size were usually smaller than ours, and most analyses were restricted to a specific setting. 8 , 9 , 10 , 11 , 12 , 13 Even in such populations we can notice different prevalence of mutations like 103N. Toledo et al evaluated a sample of 467 genotypic tests in Parana state, and detected a 46% prevalence of 103N mutation, while Westin et al, in Minas Gerais state found a lower prevalence value (32%).…”

Section: Discussionmentioning

confidence: 99%