Prevalence of reproductive tract infections among women preparing to conceive in Chongqing, China: trends and risk factors

Li, Jun; Zeng, Mengyao; Yang, Liu; Mao, Yanyan; He, Yang; Li, Min; Chen, Qing; Zhou, Weijin; Chen, Liang; Zhu, Qianxi

doi:10.1186/s12978-022-01502-x

Cited by 9 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most common pathogens are E. coli (31.5%), Enterobacter (18.4%), K. pneumoniae (12.5%), Enterococcus faecalis (11.6%), Staphylococcus aureus (10.2%), and Pseudomonas aeruginosa (8.4%). The study identified a significant association between health care-related female reproductive tract infections and a history of miscarriage (18). However, no association was found between HAIs and a history of preterm births (19).…”

Section: Discussionmentioning

confidence: 86%

See 1 more Smart Citation

Second-trimester miscarriage caused by recurrent Klebsiella pneumoniae infection: a case report

et al. 2022

View full text Add to dashboard Cite

Background: Klebsiella pneumoniae (K. pneumoniae) can cause hospital-and community-acquired pneumonia, and urinary tract, wound, and blood infections. As there are few reports on K. pneumoniae infections in pregnancy and no treatment guidelines, diagnosis and treatment are difficult. The diagnosis and treatment require a bacterial culture to confirm the diagnosis. Therefore, the condition is often exacerbated due to a lack of timely medication.Case Description: We report a case of a pregnant woman with recurrent K. pneumoniae infection during pregnancy. The 40-year-old woman was admitted to hospital at 14 weeks gestation due to fever of unknown origin. She was treated with empiric antibiotics, and her fever resolved within 1 day. A blood culture showed K. pneumoniae infection. She was discharged after 11 days of treatment. However, 10 days later, she was re-hospitalized due to fever, and treated with cefoperazone sodium and sulbactam sodium. Her fever resolved within 1 day. A blood culture again showed K. pneumoniae infection. On day 5, she experienced chills and a miscarriage. Cervical secretions showed K. pneumoniae, and a placental examination revealed chorioamnionitis. The treatment was changed to meropenem, and the patient recovered within 2 weeks.Conclusions: When a fever of unknown origin occurs during pregnancy, one should be wary of K. pneumoniae recurrence or secondary infection, and use sensitive antibiotics early. When K. pneumoniae is cultivated, the course of treatment must be sufficient, and the source of infection must be actively searched to prevent secondary infections, such as kidney cysts, liver cysts, lung cysts, and community infections. Finding the cause and taking appropriate treatment can prevent the occurrence of adverse pregnancy and childbirth history.

show abstract

Section: Discussionmentioning

confidence: 86%

“…Our literature search identified only 1 similar case reported in recent years (18) of AC, PPROM, and early second-trimester miscarriage caused by K. pneumoniae infection during pregnancy. In that case, a 36-year-old mother was admitted at 18 weeks + 1 day of gestation with threatened abortion.…”

Section: Discussionmentioning

confidence: 99%

Second-trimester miscarriage caused by recurrent Klebsiella pneumoniae infection: a case report

et al. 2022

View full text Add to dashboard Cite

show abstract

“…According to the results of a cross‐sectional study in Fujian and Sichuan Provinces, China, the prevalences of T. vaginalis were 3.2% and 0.7% (Chen et al, 2006; Yongjun et al, 2009), respectively. In addition, in a multicenter cross‐sectional study conducted in Chongqing, China, the prevalence of T. vaginalis was 0.49% among the 439,372 women of reproductive age (Liu et al, 2022). Therefore, efficient and accurate diagnostic tools are essential for mass population screening and control of T. vaginalis (Li et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene

Deng

Sheng

et al. 2023

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Trichomoniasis is a common and curable sexually transmitted disease worldwide. The rapid, convenient, and accurate diagnosis of trichomoniasis is an important link in the prevention and treatment of the disease. The current detection methods of Trichomonas vaginalis are mainly wet mount microscopy, culture, nested PCR, and loop‐mediated isothermal amplification. However, these detection methods have some shortcomings. In this study, a recombinant enzyme polymerase amplification (RPA) assay had been conducted to detect T. vaginalis. The target gene and the corresponding primers were screened, and the reaction system and conditions were optimized in the assay of RPA. The sensitivity and specificity of this detection method were analyzed. The detection efficiency of wet mount microscopy, culture, nested PCR, and RPA was compared by testing 53 clinical samples from vaginal secretions. By screening, the actin gene of T. vaginalis could be used as a target gene for RPA detection of T. vaginalis, and the optimum reaction condition to amplify the actin gene by RPA was at 39°C for 30 min. The detection limit of T. vaginalis DNA using RPA was 1 pg, corresponding to a sensitivity of approximately five trophozoites. The RPA assay demonstrated high specificity for T. vaginalis, and there was no cross‐reactivity with Giardia lamblia, Escherichia coli, Lactobacillus, Toxoplasma gondii, Staphylococcus aureus, and Candida albicans. Of the 53 clinical samples, the positive rates of T. vaginalis detected by wet mount microscopy, culture, nested PCR and RPA were 50.9 4% (27/53), 71.7% (38/53), 71.7% (38/53), and 69.81% (37/53), respectively. Compared with culture which was used as the gold standard for diagnosing trichomoniasis, testing clinical samples by wet mount microscopy showed 71.05% sensitivity, 100% specificity, and moderate diagnostic agreement with the culture (K = 0.581, Z = 4.661, p < 0.001). The nested PCR showed 100% sensitivity, 100% specificity, and excellent diagnostic agreement (K = 1, Z = 7.28, p < 0.001), while RPA displayed 97.37% sensitivity, 100% specificity, and excellent diagnostic agreement (K = 0.954, Z = 6.956, p < 0.001). At the present study, rapid amplification of actin gene by RPA could be used as a tool for detection of T. vaginalis. The detection method of RPA was more sensitive than wet mount microscopy and displayed excellent specificity. Moreover, RPA amplification of actin gene did not require a PCR instrument and the amplification time was shorter than that of ordinary PCR. Therefore, the RPA assay was proposed in this study as a point‐of‐care examination and a diagnostic method of T. vaginalis infection, which exhibited the potential value in the treatment and prevention of trichomoniasis.

show abstract

“…As the controls were recruited based on the absence of symptoms of vaginitis, it can be safely assumed that the isolation of Candida in this subset suggested colonization rather than true infection. Studies done elsewhere have reported different rates of infection ( 6 , 11 – 13 ). Multiple factors like geographical location, socioeconomic status of patients, underlying factors and methods of testing can affect the prevalence rates between studies.…”

Section: Discussionmentioning

confidence: 99%

Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India

Bashir

Altaf

Khurshid

et al. 2023

IJM

View full text Add to dashboard Cite

Background and Objectives: Vulvovaginal candidiasis (VVC) is one of the most frequent reasons for gynecological consul- tations. Candida albicans is responsible in the majority of cases. Lately, VVC caused by non-albicans Candida spp. (NAC), which are resistant to routinely used antifungals, is on the rise. This study was designed to determine the prevalence of Candida in patients suffering from vaginitis and to assess the predisposing factors along with identification of Candida species and evaluation of their susceptibility profile. Materials and Methods: High vaginal swabs were collected from 225 women. Sample processing consisted of Gram stain and culture onto Sabouraud’s dextrose agar and HiChrom Candida Differential agar. Isolates were identified and speciated using VITEK2 Compact System. Susceptibility testing was done using VITEK2 AST-Y S08 cards and disc diffusion. Results: Candida spp. were isolated from 94 (41.8%) of the cases. C. albicans was the predominant species (71.6%) fol- lowed by other NAC spp. (28.4%). Pregnancy and diabetes were the most frequently implicated risk factors (67.1% and 44.4%). High resistance was observed in NAC spp. as opposed to C. albicans to all antifungal agents tested. Conclusion: Empirical therapy with routinely used antifungals can be initiated for C. albicans. In the case of NAC spp., identification should be followed by susceptibility testing.

show abstract

Prevalence of reproductive tract infections among women preparing to conceive in Chongqing, China: trends and risk factors

Cited by 9 publications

References 23 publications

Second-trimester miscarriage caused by recurrent Klebsiella pneumoniae infection: a case report

Second-trimester miscarriage caused by recurrent Klebsiella pneumoniae infection: a case report

Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene

Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India

Contact Info

Product

Resources

About