Prevalence of renal lesions in slaughtered cattle in Shiraz, Iran, and detection of Leptospira in them by nested PCR-RFLP

Taghadosi, Vahideh; Hosseinzadeh, Saeid; Shekarforoush, Seyed Shahram; Samiei, Azadeh

doi:10.1007/s11250-016-1145-z

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…As the technique uses two sets of primers instead of one, specificity increases by reducing background due to non-specific amplification of DNA. The methodology was also applied using other targets such as lipL32 (Bomfim et al, 2008;Fraune et al, 2013), 16S rRNA (Faber et al, 2000;Kim et al, 2006;Taghadosi et al, 2016) and flab (Koizumi et al, 2013;Gamage et al, 2014;Villanueva et al, 2016).…”

Section: Accepted Articlementioning

confidence: 99%

An overview on the molecular diagnosis of animal leptospirosis

Azevedo

Lilenbaum

2021

Lett Appl Microbiol

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The most common presentation of animal leptospirosis is the subclinical and silent chronic form, that can lead to important reproductive disorders. The diagnosis of this chronic form remains a challenge. The aim of the present study is to gather and critically analyse the current information about molecular tools applied to animal leptospirosis diagnosis, particularly the silent chronic presentation of the infection. Regarding clinical specimens, samples from urinary tract were the most used (69/102, 67·7%), while few studies (12/102, 11·8%) investigated samples from reproductive tract. Concerning the molecular methods applied, the most used is still the conventional polymerase chain reaction (PCR) (46/102, 45%), followed by real‐time PCR (38/102, 37·2%). The lipL32 gene is currently the most common target used for Leptospira detection, with 48% of studies applying this genetic marker. From all the studies, only few (21/102, 20·5%) performed gene sequencing. According to the majority of authors, current evidence suggests that lipL32‐PCR is useful for an initial screening for Leptospira DNA detection in animal clinical samples. Posteriorly, if DNA sequencing could be performed on positive lipL32‐PCR samples, we encourage the use of secY gene as a genetic marker. The molecular methods appear as the most important tools for the diagnosis of the chronic silent leptospirosis on domestic animals, reinforcing its evident impact not only on animal reproduction but also on a One Health context.

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Section: Accepted Articlementioning

confidence: 99%

An overview on the molecular diagnosis of animal leptospirosis

Azevedo

Lilenbaum

2021

Lett Appl Microbiol

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“…For instance, sick animals and animals that die on farms are not sent to abattoirs and abattoir populations generally consist of healthy animals which may be of uniform age. Recent examples in the literature demonstrate abattoir surveys used to derive an abattoir prevalence rather than a target population prevalence (Borji et al, 2012;Byrne et al, 2016;Taghadosi et al, 2016). However, abattoir populations may or may not include a representative sample of either the infected animals or the at risk population.…”

Section: Discussionmentioning

confidence: 99%

A protocol to identify and minimise selection and information bias in abattoir surveys estimating prevalence, using Fasciola hepatica as an example

Carroll

Forbes

Graham

et al. 2017

Preventive Veterinary Medicine

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Abattoir surveys and findings from post-mortem meat inspection are commonly used to estimate infection or disease prevalence in farm animal populations. However, the function of an abattoir is to slaughter animals for human consumption, and the collection of information on animal health for research purposes is a secondary objective. This can result in methodological shortcomings leading to biased prevalence estimates. Selection bias can occur when the study population as obtained from the abattoir is not an accurate representation of the target population. Virtually all of the tests used in abattoir surveys to detect infections or diseases that impact animal health are imperfect, leading to errors in identifying the outcome of interest and consequently, information bias. Examination of abattoir surveys estimating prevalence in the literature reveals shortcomings in the methods used in these studies. While the STROBE-Vet statement provides clear guidance on the reporting of observational research, we have not found any guidelines in the literature advising researchers on how to conduct abattoir surveys. This paper presents a protocol in two flowcharts to help researchers (regardless of their background in epidemiology) to first identify, and, where possible, minimise biases in abattoir surveys estimating prevalence. Flowchart 1 examines the identification of the target population and the appropriate study population while Flowchart 2 guides the researcher in identifying, and, where possible, correcting potential sources of outcome misclassification. Examples of simple sensitivity analyses are also presented which approximate the likely uncertainty in prevalence estimates due to systematic errors. Finally, the researcher is directed to outline any limitations of the study in the discussion section of the paper. This protocol makes it easier to conduct an abattoir survey using sound methods, identifying and, where possible, minimizing biases.

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“…Serological surveys in cattle at slaughter vary from 3% to 80% [ 241 , 242 , 243 , 244 , 245 , 246 ] among studies. Thus, seroprevalence of around 80% in cattle in which 20% of them were PCR positive in kidney samples [ 245 ].…”

Section: Surveillance Of Zoonotic Agents In the Slaughterhousementioning

confidence: 99%

The Importance of the Slaughterhouse in Surveilling Animal and Public Health: A Systematic Review

García‐Díez

Saraiva

Moura

et al. 2023

Veterinary Sciences

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From the point of public health, the objective of the slaughterhouse is to guarantee the safety of meat in which meat inspection represent an essential tool to control animal diseases and guarantee the public health. The slaughterhouse can be used as surveillance center for livestock diseases. However, other aspects related with animal and human health, such as epidemiology and disease control in primary production, control of animal welfare on the farm, surveillance of zoonotic agents responsible for food poisoning, as well as surveillance and control of antimicrobial resistance, can be monitored. These controls should not be seen as a last defensive barrier but rather as a complement to the controls carried out on the farm. Regarding the control of diseases in livestock, scientific research is scarce and outdated, not taking advantage of the potential for disease control. Animal welfare in primary production and during transport can be monitored throughout ante-mortem and post-mortem inspection at the slaughterhouse, providing valuable individual data on animal welfare. Surveillance and research regarding antimicrobial resistance (AMR) at slaughterhouses is scarce, mainly in cattle, sheep, and goats. However, most of the zoonotic pathogens are sensitive to the antibiotics studied. Moreover, the prevalence at the slaughterhouse of zoonotic and foodborne agents seems to be low, but a lack of harmonization in terms of control and communication may lead to underestimate its real prevalence.

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Prevalence of renal lesions in slaughtered cattle in Shiraz, Iran, and detection of Leptospira in them by nested PCR-RFLP

Cited by 7 publications

References 22 publications

An overview on the molecular diagnosis of animal leptospirosis

An overview on the molecular diagnosis of animal leptospirosis

A protocol to identify and minimise selection and information bias in abattoir surveys estimating prevalence, using Fasciola hepatica as an example

The Importance of the Slaughterhouse in Surveilling Animal and Public Health: A Systematic Review

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