Prevalence of puroindoline alleles in wheat varieties from eastern Asia including the discovery of a new SNP in puroindoline b

Tanaka, Hiroyuki; Morris, Craig F.; Haruna, Mika; Tsujimoto, Hisashi

doi:10.1017/s1479262108993151

Cited by 15 publications

(13 citation statements)

References 40 publications

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“…Tauschii hard durum wheat. Other reports of Pinb deletions (Ikeda et al 2005;Chang et al 2006;Tanaka et al 2007) also involve Pina and are mentioned earlier. There appears to be only one report of a deletion in Pinb alone: the variety Jinguangmai with Pina-D1a/Pinb null haplotype (Chang et al 2006).…”

Section: Puroindoline B Alleles In Common Wheatmentioning

confidence: 64%

“…This mutation has now been reported in numerous studies worldwide (Cane et al 2004;Xia et al 2005;Ikeda et al 2005;Chang et al 2006;Chen et al 2006;Lillemo et al 2006;Pickering and Bhave 2007;Tanaka et al 2007). …”

Section: Puroindoline B Alleles In Common Wheatmentioning

confidence: 80%

“…A more complete analysis of this deletion is required since it appears to contradict the results of Morris et al (2001a) which reported an accession of Red Egyptian to have a Pina-D1b/Pinb-D1b haplotype and a SKCS value in the range typical of hard common wheats. Ikeda et al (2005), Chang et al (2006) and Tanaka et al (2007) also reported wheat cultivars with a 'double-null' mutation of both Pina-D1 and Pinb-D1 genes, again possibly indicating a complete deletion of the Ha locus. It is unclear whether these reports identify the same large deletion as reported by Tranquilli et al (2002).…”

Section: Biochemical Properties Of Puroindoline Proteinsmentioning

confidence: 89%

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Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses

2007

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The hardness or texture of cereal grains is a primary determinant of their technological and processing quality. Among members of the Triticeae, most notably wheat, much of the variation in texture is controlled by a single locus comprised of the Puroindoline a, Puroindoline b and Grain Softness Protein-1 (Gsp-1) genes. Puroindolines confer the three major texture classes of soft and hard common wheat and the very hard durum wheat. The protein products of these genes interact with lipids and are associated with the surface of isolated starch (as a protein fraction known as 'friabilin'). During the past ten years a great diversity of alleles of both Puroindoline genes have been discovered and significant advances made in understanding the relationship between the gene presence/absence, sequence polymorphism and texture of cereal grains. Efforts have also focussed on Puroindoline and Gsp-1 genes in diploid progenitors, other Triticeae grasses and synthetic wheats in order to understand the evolution of this gene family and find potentially useful variants. The puroindoline homologues in other cereals such as rye and barley are also receiving attention. This work summarises new developments in molecular genetics of puroindolines in wheat and related Triticeae grasses, and the related genes in other cereals.

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Section: Puroindoline B Alleles In Common Wheatmentioning

confidence: 64%

Section: Puroindoline B Alleles In Common Wheatmentioning

confidence: 80%

Section: Biochemical Properties Of Puroindoline Proteinsmentioning

confidence: 89%

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Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses

2007

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“…Puroindoline allele frequencies were reported among wheat varieties from Europe, US, Canada and Latin American (Lillemo and Morris 2000), North America , India and Kansas (Ram et al 2002), Portugal (Bagulho et al 2003), southern Australia (Cane et al 2004), Europe (Huang and Röder 2005), Chinese breeding programs (Xia et al 2005), China (Chang et al 2006, 2010b, CIMMYT , Asia (Tanaka et al 2008), and various other variety collections (Ikeda et al 2005;Ravel et al 2006;Chen et al 2007;Pickering and Bhave 2007). In total, seven hardness alleles were found and PinbD1b,c,d,e,f,p).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Puroindoline D1 and Puroindoline b-2 variants in U.S. Pacific Northwest wheat breeding germplasm pools, and their association with kernel texture

Geng

Beecher

et al. 2012

Theor Appl Genet

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Kernel texture is a major factor influencing the classification and end use properties of wheat (Triticum aestivum L.), and is mainly controlled by the Puroindoline a (Pina) and Puroindoline b (Pinb) genes. Recently, a new puroindoline gene, Puroindoline b-2 (Pin b-2), was identified. In this study, 388 wheat cultivars and advanced breeding lines from the U.S. Pacific Northwest were investigated for frequencies of Puroindoline D1 alleles and Pinb-2 variants 2 and 3. Results indicated that Pinb-D1b (74.0%) was the predominant genotype among hard wheats (N = 196), the only other hard allele encountered was Pina-D1b (26.0%). Across all varieties, Pinb-2v3 was the predominant genotype (84.5%) compared with Pinb-2v2 (15.5%). However, among 240 winter wheat varieties (124 soft white, 15 club, 68 hard red and 33 hard white varieties), all carried Pinb-2v3. Among spring wheats, Pinb-2v2 and Pinb-2v3 frequencies were more variable (soft white 25.0:75.0, hard red 58.2:41.8 and hard white 40.0:60.0, respectively). Kernel texture variation was analyzed using 247 of the 388 wheat varieties grown in multi-location factorial trials in up to 7 crop years. The range of variety means among the four groups, soft winter, soft spring, hard winter and hard spring, was on the order of 15-25 single kernel characterization system (SKCS) Hardness Index. The least significant difference for each of these trials ranged from 2.8 to 5.6 SKCS Hardness Index. Observations lead to the conclusion that Pinb-2 variants do not exert a prominent effect on kernel texture, however, Pinb-2 variants do identify features of wheat germ plasm structure in the U.S. Pacific Northwest.

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“…To date, 9 and 17 alleles are identiWed at the Pina and Pinb loci, respectively Chen et al 2005Chen et al , 2006Chen et al , 2007aGazza et al 2005;Morris 1997, 1998;Ikeda et al 2005;Li et al 2007;Lillemo and Morris 2000;Morris et al 2001; Morris and Bhave 2008;Ram et al 2002Ram et al , 2005Tanaka et al 2007;Xia et al 2005). DiVerent alleles of Pina and Pinb generate diVerent grain textures, and accordingly have diVerent eVects on milling and food processing quality (Cane et al 2004;Eagles et al 2006;Giroux et al 2000;Ikeda et al 2005;Martin et al 2001;Nagamine et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Pina and Pinb allelic variations in Xinjiang landraces and commercial wheat cultivars

Wang

Xia

et al. 2008

Euphytica

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Grain hardness plays an important role in determining both milling performance and quality of the end-use products produced from common or bread wheat. The objective of this study was to characterize allelic variations at the Pina and Pinb loci in Xinjiang wheat germplasm for further understanding the mechanisms involved in endosperm texture formation, and the status of grain texture in Chinese bread wheat. A total of 291 wheat cultivars, including 56 landraces, and 95 introduced and 140 locally improved cultivars, grown in Xinjiang, were used for SKCS measurement and molecular characterization. Among the harvested grain samples, 185 (63.6%), 40 (13.7%), and 66 (22.7%) were classiWed as hard, mixed and soft, respectively. Eight diVerent genotypes for the Pina and Pinb loci were identiWed, including seven previously reported genotypes, viz., Pina-D1a/Pinb-/Pinb-D1ab, and a novel Pinb allele, PinbD1ac. This new allele, detected in Kashibaipi (local landrace) and Red Star (from Russia) has a double mutation at the 257th (G to A substitution) and 382nd (C to T substitution) nucleotide positions of the coding region. Pina-D1b, Pinb-D1b, and Pinb-D1p were the most common alleles in Xinjiang wheat germplasm, with frequencies of 14.3%, 38.1% and 28.6% in hard textured landraces, 25.5%, 56.9% and 11.8% in hard introduced cultivars, and 24.8%, 47.8% and 26.5% in hard locally improved cultivars, respectively. The restriction enzymes ApaI, SapI, BstXI and SfaNI were used to identify Pinb-D1ab or Pinb-D1ac, Pinb-D1b, Pinb-D1e and Pinb-Dg, respectively, by digesting PCR products of the Pinb gene. The unique grain hardness distribution in Xinjiang bread wheat, as well as the CAPs markers for identiWcation of the Pinb alleles provided useful information for breeding wheat cultivars with optimum grain textures.

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Prevalence of puroindoline alleles in wheat varieties from eastern Asia including the discovery of a new SNP in puroindoline b

Cited by 15 publications

References 40 publications

Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses

Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses

Prevalence of Puroindoline D1 and Puroindoline b-2 variants in U.S. Pacific Northwest wheat breeding germplasm pools, and their association with kernel texture

Molecular characterization of Pina and Pinb allelic variations in Xinjiang landraces and commercial wheat cultivars

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