Prevalence of positivity of CBNAAT(cartridge based nucleic acid amplification test) in extra-pulmonary tuberculosis.

Abstract

Original Research Article Introduction: Tuberculosis (TB) remains a key challenge in the face of global public health and inadequate diagnostic assays have hampered our chances to tackle this disease effectively. The Gene X-pert MTB/RIF assay marks an important development in the field of rapid molecular TB diagnostics. This study was done to see the prevalence of gene Xpert MTB/RIF assay and drug resistant tuberculosis in extrapulmonary tuberculosis. Material and methods: The 300 samples of extra-pulmonary tuberculosis constituting pleural fluid, pus, CSF, lymph tissue, peritoneal fluid diagnosed on the basis of clinico radiological and/or histopathological findings were sent for gene Xpert MTB/RIF. Results: Maximum number of patients in our study are tubercular pleural effusion (67.7%) followed by tubercular lymphadenitis, empyema, Cerebrospinal fluid, ascitic fluid and pericardial fluid. Mtb had been detected in pleural effusion (13.8%), lymphadenitis (60.3%), empyema (44.4%) and ascitic fluid (11.1%). 16.7% cases were diagnosed as multi drug resistant-extrapulmonary tuberculosis (MDR-EPTB). Conclusion: Gene Xpert assays a more sensitive technique as compared to conventional methods. The Gene X-pert MTB/RIF assay marks an important development in the field of rapid molecular TB diagnostics.

Prevalence of Extrapulmonary Tuberculosis in Newly Registered HIV Patients

“…Another study conducted by Mahesh Gupta et al on prevalence of positivity of CBNAAT in EPTB patients included 300 patients (203 pleural fluid, 58 lymph node aspirate, 18 empyema, 9 ascitic fluid, 9 CSF, 3 pericardial fluid) and diagnosed EPTB on history, clinical and radiological basis. 15 Out of these 28 (13.8%) patients of pleural fluid, 35 (60.3%) patients of lymph node aspirate, 8 (44.4%) patients of empyema, 1 (11.1%) patient of ascitic fluid and none of the CSF and pericardial fluid were CBNAAT positive and out of 72 positive patients 12 (16.7%) were rifampicin resistant. This study concluded that Gene Xpert assay has the potential to significantly improve and escalate the diagnosis from smear negative body fluids in the regions with not only high TB burden but also with overlapping HIV.…”