Prevalence of Plasmid-Mediated Quinolone Resistance in &lt;i&gt;Escherichia coli&lt;/i&gt; Isolates in Wenzhou, Southern China, 2002-2008

Zhou, Tong; Chen, Xuejun; Zhou, Mingming; Zhao, Yujie; Luo, Xin; Bao, Qiyu

doi:10.7883/yoken.64.55

Cited by 23 publications

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“…The combined detection with total DNA and individual isolate plasmid DNA was an effective way to avoid missing the presence of the resistant genes in the DNA of anaerobic bacteria, autotrophic bacteria, and many uncultured bacteria. There was a higher detection frequency of quinolones-resistant genes (96.7 %) than in Escherichia coli isolates from river water (35.2 %) (Sun et al 2012), urban water (35 %) (Zhou et al 2011) and swine farm wastewater (58.3 %) (Liu et al 2008). It appears that there is a great potential risk of bacterial resistance to quinolones in pharmaceutical sewage.…”

Section: Discussionmentioning

confidence: 95%

Analysis of the structure of bacteria communities and detection of resistance genes of quinolones from pharmaceutical wastewater

Zhang

Meng

et al. 2013

Ann Microbiol

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The aim of the present study was to investigate the distribution of bacteria and detect the presence of quinolone resistance gene (qnrA) and integrons (intI1, intI2) in a habitat polluted by pharmaceutical sewage. The bacteria were isolated by nutrient agar and nutrient broth from waste water and sludge collected from the sewage outfall of a pharmaceutical factory. The bacteria were identified by Gram staining and biochemical tests, and the bacterial community diversity was analyzed by Shannon-Wiener diversity index (H), Pielou evenness index (J) and Simpson's diversity index (D). The occurrence of qnrA and integrons (intI1, intI2) were detected by Real-time PCR assays. The results showed that 90 strains were isolated from water samples and sludge samples including 22 genera and 26 species. Types of bacteria in water samples contained 18 genera and 20 species, while 13 genera and 14 species were detected in sludge samples. Fifty-five Enterobacteriaceae isolates (61.11 %, 55 of 90) were the predominant bacteria in water and sludge samples. Bacterial species richness and evenness in water samples were higher than in sludge samples. The resistance genes of qnrA and integrons (intI1, intI2) with the total DNA and single isolate plasmid DNA were detected. There were a variety of bacterial species and the presence of qnrA and integrons (intI1, intI2) genes in pharmaceutical wastewater habitats, in which Enterobacteriaceae strains were the dominant bacteria. These results suggested that pharmaceutical wastewater had potential risks to public health.

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Section: Discussionmentioning

confidence: 95%

Analysis of the structure of bacteria communities and detection of resistance genes of quinolones from pharmaceutical wastewater

Zhang

Meng

et al. 2013

Ann Microbiol

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“…Resistance to quinolones arises mainly through chromosomal mutations and plasmids. Topoisomerase IV and DNA gyrase enzyme Qnr proteins include qnrA, qnrB, qnrC, qnrD and qnrS and belong to the family of penteptide proteins that lead to resistance to quinolones ( 12 , 13 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of main quinolones and carbapenems resistance genes in clinical and veterinary Escherichia coli strains

Karshenas¹,

Salehi²,

Adabi³

et al. 2022

IJM

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Background and Objectives: Antibiotics-resistant Escherichia coli strains are considered one of the most important causes of human and animal infectionsworldwide. The aim of current study was to detect common resistance (carbapenems and quinolones) genes by PCR. Materials and Methods: A total of 100 E. coli strains isolated from human urinary tract infection and 20 isolated strains of aborted sheep embryos were collected. PCR was performed using specific primers to detect the resistance genes. Results: Overall, among the quinolones resistance genes, qnrS resistance gene had the highest frequency (48%) and among carbapenem resistance genes, impresistance gene had the highest frequency (45%). The frequency of resistance genes, IMP (28.45%), KPC (9.5%), VIM (9.15%), NDM (7.20%) were observedin clinical and veterinary strains, respectively. Accord- ing to the results, 38.6% of E. coli strains had at least one from five genes of resistance to quinolones.The lowest frequency of resistance gene was related to qnrA, which was observed in only 29 (24.2%) strains. Conclusion: Monitoring of carbapenem and quinolone resistance in pathogenic E. coli to humans and animals has an im- portant value in revising treatment guidelines and the national public health, and plays an important role in preventing the spread of resistant strains.

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Occurrence of virulence genes, 16S rRNA methylases, and plasmid-mediated quinolone resistance genes in CTX-M-producing Escherichia coli from Pakistan

Habeeb

Haque

Iversen

et al. 2013

Eur J Clin Microbiol Infect Dis

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The aim of the study was to conduct a comprehensive molecular characterization of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli collected from Pakistan. Genetic relatedness among 98 ESBL-producing E. coli was measured by pulsed-field gel electrophoresis (PFGE). The presence of genes encoding ESBLs, virulence factors (VFs), 16S rRNA methylases, plasmid-mediated quinolone resistance (PMQR) encoding elements, plasmid replicon types, phylogenetic groups of E. coli, prevalence of the worldwide disseminated clone E. coli ST131, and phylogrouping of CTX-M enzymes was investigated by polymerase chain reaction (PCR). All isolates carried bla CTX-M genes and, except for one isolate from CTX-M phylogroup 9, they all belonged to CTX-M phylogroup 1. The isolates were genetically diverse with PFGE. Phylogenetic group D (36 %) was most abundant in this collection of E. coli, whereas isolates belonging to B2 (22 %) had the highest content of virulence genes. PMQR genes were found in 84.6 % of the isolates; among them, 93 % isolates were positive for variants of acetyltransferases (aac(6')-lb-cr), whereas qnrB, qepA, and qnrS were present in 11 %, 5 %, and 4 % of the isolates, respectively. Only 3 % of the isolates contained genes encoding 16S rRNA methylases. The most abundant replicon type was IncF (96 %), and 18 % of the isolates belonged to the ST131 clone. Out of 34 investigated VFs, 24 genes encoding different types of adhesins, protectins, toxins, siderophores, and other VFs were found. Although the isolates in this collection were highly resistant to many antimicrobials, susceptibility to amikacin and meropenem was retained.

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Prevalence of Plasmid-Mediated Quinolone Resistance in <i>Escherichia coli</i> Isolates in Wenzhou, Southern China, 2002-2008

Cited by 23 publications

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Analysis of the structure of bacteria communities and detection of resistance genes of quinolones from pharmaceutical wastewater

Analysis of the structure of bacteria communities and detection of resistance genes of quinolones from pharmaceutical wastewater

Prevalence of main quinolones and carbapenems resistance genes in clinical and veterinary Escherichia coli strains

Occurrence of virulence genes, 16S rRNA methylases, and plasmid-mediated quinolone resistance genes in CTX-M-producing Escherichia coli from Pakistan

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