Prevalence of pathogenicity island markers genes in uropathogenic Escherichia coli isolated from patients with urinary tract infectious

Tangi, Sima Chahar; Tajbakhsh, Elahe; Soleimani, Nazila Arbab; Shahraki, Manochehr Momeni

doi:10.1016/s2222-1808(15)60909-4

Cited by 9 publications

(9 citation statements)

References 24 publications

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“…Of the two PAIs detected, PAI IV536 also known as high pathogenicity Island (HPI) was substantially dominant with a genotypic frequency of 71.7% and PAI IICFT073 had a frequency of 28.3%. This is in agreement with previous studies [24,60] which reported PAI IV536 as the most prevalent PAI. The main virulence genes residing in the PAI IV536 and PAI IICFT073 are yersiniabactin siderophore iron-uptake system and P. mbriae as well as iron regulated proteins respectively [61,63,64].…”

Section: Discussionsupporting

confidence: 94%

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Carbapenem resistance profiles of Pathogenic Escherichia coli in Uganda

Ssekatawa

Byarugaba

Nakavuma

et al. 2020

Preprint

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Background: Escherichia coli has been implicated as one of the main etiological agents of diarrhea, urinary tract infections, meningitis and septicemia worldwide. The ability to cause diseases is potentiated by presence of virulence factors. The virulence factors influence the capacity of E. coli to infect and colonize different body systems. Thus, pathogenic E. coli are grouped into DEC strains that are mainly clustered in phylogenetic group B1 and A; ExPEC belonging to A, B2 and D. Coexistence of virulence and beta-lactamase encoding genes complicates treatment outcomes. Therefore, this study aimed at presenting the CR profiles among pathogenic E. coli. Methods: This was a retrospective cross-sectional study involving use of archived E. coli clinical isolates collected in 2019 from four Ugandan tertiary hospitals. The isolates were subjected to antibiotics sensitivity assays to determine phenotypic resistance. Four sets of multiplex PCR were performed to detect CR genes, DEC pathotypes virulent genes, ExPEC PAI and the E. coli phylogenetic groups. Results:Antibiotic susceptibility revealed that all the 421 E. coli isolates used were MDR as they exhibited 100% resistance to more than one of the first-line antibiotics. The study registered phenotypic and genotypic CR prevalence of 22.8% and 33.0% respectively. The most predominant gene was blaOXA-48 with genotypic frequency of 33.0%, then blaVIM(21.0%), blaIMP(16.5%), blaKPC(14.8%) and blaNDM(14.8%). Spearman’s correlation revealed that presence of CR genes was highly associated with phenotypic resistance. Furthermore, of 421 MDR E. coli isolates, 19.7% harboured DEC virulent genes, where EPEC recorded significantly higher prevalence (10.8%) followed by S-ETEC(3.1%), STEC(2.9%), EIEC (2.0%) and L-ETEC(2.0%). Genetic analysis characterized 46.1% of the isolates as ExPEC and only PAI IV536(33.0%) and PAI IICFT073(13.1%) were detected. Phylogenetic group B2 was predominantly detected (41.1%), followed by A(30.2%), B1(21.6%), and D(7.1%). Furthermore, 38.6% and 23.1% of the DEC and ExPEC respectively expressed phenotypic resistance.Conclusion: Our results exhibited significant level of CR carriage among the MDR DEC and ExPEC clinical isolates belonging to phylogenetic groups B1 and B2 respectively. Virulence and CR genetic factors are mainly located on mobile elements. Thus, constitutes a great threat to the healthcare system as it promotes horizontal gene transfer.

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Section: Discussionsupporting

confidence: 94%

“…In this study, multiplex PCR was used to target Pathogenicity Islands (PAI). PAIs harbour virulent genes in ExPEC that are responsible for pathogenicity [60][61][62].…”

Section: Discussionmentioning

confidence: 99%

Carbapenem resistance profiles of Pathogenic Escherichia coli in Uganda

Ssekatawa

Byarugaba

Nakavuma

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…PAIs structurally consist of a sequence of DNA (< 10 kb) in size. They encode mobility genes (e.g., integrases and insertion sequences), and are generally associated with tRNA genes, flanked by short repeated sequences that have different guanine and cytosine content from the bacterial core genome [9,10]. PAIs were reported in UPEC strain 536 for the first time [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Firoozeh

Soleimani-Moorchekhorti

Zibaei

2017

J Infect Dev Ctries

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Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs) .The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536 were not detected in the UPEC strains. Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.

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“…In this study, multiplex PCR was used to target Pathogenicity Islands (PAI). PAIs harbour virulent genes in ExPEC that are responsible for pathogenicity [60][61][62]. The overall prevalence of ExPEC as revealed by molecular typing of PAI in our study was 46.1% (194/421).…”

Section: Discussionmentioning

confidence: 52%

Carbapenem Resistance Profiles of Pathogenic Escherichia coli in Uganda

Ssekatawa

Byarugaba

Nakavuma

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Escherichia coli has been implicated as one of the main etiological agents of diarrhea, urinary tract infections, meningitis and septicemia worldwide. The ability to cause diseases is potentiated by presence of virulence factors. The virulence factors influence the capacity of E. coli to infect and colonize different body systems. Thus, pathogenic E. coli are grouped into DEC strains that are mainly clustered in phylogenetic group B1 and A; ExPEC belonging to A, B2 and D. Coexistence of virulence and beta-lactamase encoding genes complicates treatment outcomes. Therefore, this study aimed at presenting the CR profiles among pathogenic E. coli. Methods: This was a retrospective cross-sectional study involving use of archived E. coli clinical isolates collected in 2019 from four Ugandan tertiary hospitals. The isolates were subjected to antibiotics sensitivity assays to determine phenotypic resistance. Four sets of multiplex PCR were performed to detect CR genes, DEC pathotypes virulent genes, ExPEC PAI and the E. coli phylogenetic groups. Results: Antibiotic susceptibility revealed that all the 421 E. coli isolates used were MDR as they exhibited 100% resistance to more than one of the first-line antibiotics. The study registered phenotypic and genotypic CR prevalence of 22.8% and 33.0% respectively. The most predominant gene was blaOXA-48 with genotypic frequency of 33.0%, then blaVIM(21.0%), blaIMP(16.5%), blaKPC(14.8%) and blaNDM(14.8%). Spearman’s correlation revealed that presence of CR genes was highly associated with phenotypic resistance. Furthermore, of 421 MDR E. coli isolates, 19.7% harboured DEC virulent genes, where EPEC recorded significantly higher prevalence (10.8%) followed by S-ETEC(3.1%), STEC(2.9%), EIEC (2.0%) and L-ETEC(2.0%). Genetic analysis characterized 46.1% of the isolates as ExPEC and only PAI IV536(33.0%) and PAI IICFT073(13.1%) were detected. Phylogenetic group B2 was predominantly detected (41.1%), followed by A(30.2%), B1(21.6%), and D(7.1%). Furthermore, 38.6% and 23.1% of the DEC and ExPEC respectively expressed phenotypic resistance.Conclusion: Our results exhibited significant level of CR carriage among the MDR DEC and ExPEC clinical isolates belonging to phylogenetic groups B1 and B2 respectively. Virulence and CR genetic factors are mainly located on mobile elements. Thus, constitutes a great threat to the healthcare system as it promotes horizontal gene transfer.

show abstract

Prevalence of pathogenicity island markers genes in uropathogenic Escherichia coli isolated from patients with urinary tract infectious

Cited by 9 publications

References 24 publications

Carbapenem resistance profiles of Pathogenic Escherichia coli in Uganda

Carbapenem resistance profiles of Pathogenic Escherichia coli in Uganda

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Carbapenem Resistance Profiles of Pathogenic Escherichia coli in Uganda

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