Prevalence of parasites in amphipods Diporeia spp. from Lakes Michigan and Huron, USA

Messick, Gretchen A.; Overstreet, Robin M.; Nalepa, Thomas F.; Tyler, SJ

doi:10.3354/dao059159

Cited by 27 publications

(23 citation statements)

References 49 publications

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“…amphipods collected from Lakes Michigan and Huron, USA (Messick et al 2004). Presumed developmental stages and morphology including lid and spore ornamentation was observed using either transmission electron microscopy (TEM) or light microscopy.…”

Section: Introductionmentioning

confidence: 99%

Haplosporidian parasite in Diporeia spp. amphipods from the Great Lakes region, USA

Messick

2009

Dis. Aquat. Org.

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Light microscope observations of a haplosporidian in Diporeia spp. amphipods from Lakes Michigan and Huron, USA, found that the parasite spore is operculate and measures 8.1 µm in length and 6.1 µm in width. Round to amorphous sporocysts averaging 23.6 µm were present throughout hemal sinuses of infected amphipods. The number of developing spores within a sporocyst was highly variable and spores were observed in various stages of development. Electron microscopy revealed a lid over the operculum of developing spores and documented spores with fairly large and extensive ornamentation. This is the first haplosporidian reported in Diporeia spp. amphipods and only the second haplosporidian from freshwater species.KEY WORDS: Parasite · Haplosporidian · Amphipod · Diporeia spp. · Great Lakes · Microscopy Resale or republication not permitted without written consent of the publisherInter-Research 2009 · www.int-res.com Dis Aquat Org 83: [153][154][155][156][157] 2009 schizogony and maturation of spores were observed throughout the hemal sinuses of the host. Individual spores measured 8.1 µm in length (SE = 0.23) and 6.1 µm in width (SE = 0.16, n = 21). A hinged operculum was evident on individual, mature spores. A small projection was apparent on the posterior or abopercular end of several mature spores and thin filaments or extensions appeared to be present on a few mature spores.In what was presumed to be early stages of parasite development, sporonts contained numerous nuclei with small, dense endosomes (nucleoli). Parasite cytoplasm was granular and somewhat basophilic, and there was no obvious separation between nuclei in some early stage sporonts. It appears that sporoblasts developed with larger nuclei, some with acentric endosomes adjacent to the nuclear wall. Occasionally, small sporoblasts were seen individually or as sporonts with single nuclei (Fig. 2b). Sporonts were amorphous, oval or rounded in shape. Sporonts with developing sporoblasts had clear, semi-granular or granular cytoplasm.In sporoblasts that appeared more mature, the endosporoplasm was defined, dense and basophilic. In some, a translucent area was present between the endosporoplasm and spore wall (Fig. 2c). The endosporoplasm of some sporoblasts contained a secondary basophilic body. Some sporoblasts were ellipsoidal with a limiting membrane apparent and some had dense basophilic structures in the apex of the elliptical developing spore (Fig. 2d). Sporoblasts differentiated and matured into spores; a few maturing spores had what appeared to be a sculptured cell wall with a developing operculum.Some AFB-stained sporocysts had numerous round sporoblasts with knob-like projections; others had a prominent bar-shaped structure that extended 1.3 µm (SE = 0.10, n = 21) beyond the sporoplasm (Fig. 2e). The endosporoplasm of some younger spores (those that took up more AFB staining) appeared to be separating, whereas more mature spores (less staining) had a smaller AFB-positive body and faint internal structures or debris; mature spores w...

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Section: Introductionmentioning

confidence: 99%

Haplosporidian parasite in Diporeia spp. amphipods from the Great Lakes region, USA

Messick

2009

Dis. Aquat. Org.

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“…Ideally, then, such a study should be conducted in a single host-parasite system; a single system is advantageous in that it allows the researcher to assess whether all infected hosts express the same combination of modified traits at the same intensity. However, the potentially large number of PIPAs Messick et al, 2004). However, there is evidence that such parasitic organisms can affect the phenotype of their hosts and eventually interact with the effects of macroparasites (Haine et al, 2005).…”

Section: Multidimensionality Of Phenotypic Alterations In Infected Hostsmentioning

confidence: 99%

Multidimensionality in parasite-induced phenotypic alterations: ultimateversusproximate aspects

Cézilly

Favrat

Perrot‐Minnot

2013

Journal of Experimental Biology

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SummaryIn most cases, parasites alter more than one dimension in their host phenotype. Although multidimensionality in parasite-induced phenotypic alterations (PIPAs) seems to be the rule, it has started to be addressed only recently. Here, we critically review some of the problems associated with the definition, quantification and interpretation of multidimensionality in PIPAs. In particular, we confront ultimate and proximate accounts, and evaluate their own limitations. We end up by introducing several suggestions for the development of future research, including some practical guidelines for the quantitative analysis of multidimensionality in PIPAs.

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“…Lastly, it is also possible that the budding structure found in the tissue's of Diporeia may not be pathogenic and/or may be present as a commensal (Messick et al, 2004). As a consequence, more studies are needed to confirm any speculation.…”

Section: Discussmentioning

confidence: 99%

“…Rickettsia-like infection, i.e. Haplosporidia and Microsporidia, have all been observed in Diporeia tissues (Messick et al, 2004). The origin of these pathogens is not known.…”

Section: Introductionmentioning

confidence: 99%

Decline of Diporeia in Lake Michigan: Was Disease Associated With Invasive Species the Primary Factor?

Cave¹,

Strychar²

2014

IJB

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Populations of the freshwater amphipod Diporeia spp. have steadily declined in Lake Michigan since the late 1980's. Prior studies have provided inconclusive data on possible reasons for their decline. However, some authors suggest that food competition and/or diseases associated with aquatic invasive species (AIS), such as zebra mussels (Dreissena polymorpha), may have caused the collapse of Diporeia. In this project, the possibility of pathogens as the cause of the collapse of Diporeia has been examined. Linear regression modeling show a significant positive linear association between percent of Diporeia exhibiting a pathogenic infection and year (r=0.7202264, p≤0.0124). Chi-square testing for independence was also used to test if there was an association between year and percent infection (X 2 = 50, df = 10, p≤0.0001), implying significant association between year and infection. Hence, the introduction of zebra mussels and the diseases they carry may have been the root cause for the decline of Diporeia. Future research is needed to examine other invasive species for similar pathogens, including live studies showing direct causality between zebra mussels and the decline in Diporeia.

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Prevalence of parasites in amphipods Diporeia spp. from Lakes Michigan and Huron, USA

Cited by 27 publications

References 49 publications

Haplosporidian parasite in Diporeia spp. amphipods from the Great Lakes region, USA

Haplosporidian parasite in Diporeia spp. amphipods from the Great Lakes region, USA

Multidimensionality in parasite-induced phenotypic alterations: ultimateversusproximate aspects

Decline of Diporeia in Lake Michigan: Was Disease Associated With Invasive Species the Primary Factor?

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