Prevalence of ovine footrot in the tropical climate of southern India and isolation and characterisation of Dichelobacter nodosus

Sreenivasulu, D.; Vijayalakshmi, S.; Raniprameela, D.; Karthik, A.; Wani, S.A.; Hussain, I.

doi:10.20506/rst.32.2.2209

Cited by 18 publications

(14 citation statements)

References 16 publications

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“…At this time of year, the animals start migrating back towards the plains, passing through the areas where hard rough rocks predispose them to foot injuries and the sub-temperate and rainy climate of the region plays an additional role in establishing foot lesions. Similar observations have been made from the south Indian states of Andhra Pradesh and Tamil Nadu, where an increased incidence of footrot was reported during monsoon, with a complete lack of footrot during the dry periods (22). In the flocks under investigation, limping and associated symptoms of lameness were noted by the owners during the months of monsoon when the animals were on the upper elevations of the areas.…”

Section: Discussionsupporting

confidence: 80%

“…In the state of J&K, losses in sheep production as a result of footrot were estimated around Rs 36.79 million (US$0.56 million) (20) and Rs 15.82 million (US$0.25 million) (21) per annum in south Kashmir and central Kashmir, respectively. Footrot has also been reported from the south Indian states of Andhra Pradesh (22) and Kerala (23). The climatic conditions and animal husbandry practices in HP and Uttrakhand, another northern state, are very similar to those in J&K. With the expanding tracts of migration and mixing of sheep and goat flocks in common grazing pastures at the borders, the sheep and goat-rearing communities of HP remain highly vulnerable to severe economic losses due to infectious lameness.…”

Section: Introductionmentioning

confidence: 94%

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Infectious lameness among migratory sheep and goats in north-west India, with particular focus on anaerobes

Wani¹,

Verma²,

Sharma³

et al. 2015

Rev. Sci. Tech. OIE

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Various bacterial species, particularly Dichelobacter nodosus, Fusobacterium necrophorum, Trueperella pyogenes (previously Arcanobacterium pyogenes) and Treponema spp., have been implicated in infectious conditions manifesting in lameness in sheep and goat populations. The current study reports the causes of infectious lameness in the north-western Himalayan region, particularly Himachal Pradesh (HP), where no such study has been conducted in the past. Among a total surveyed population of 27,586 animals, comprised of 15,006 sheep and 12,580 goats, 216 samples were collected from foot lesions. A total of 6.48% (14/216) samples were positive for D. nodosus, 20.83% (45/216) for F. necrophorum and 20.37% (44/216) for T. pyogenes. In three instances, all of the three aforementioned bacteria were present in a single foot lesion. Most of the positive samples were from areas in the state of HP adjoining the state of Jammu and Kashmir (J&K), where footrot is endemic. The confirmation of footrot in HP and the fact that F. necrophorum and T. pyogenes were detected from cases of foot/hoof infection in high numbers emphasises that these organisms play an important role in inducing morbidity in migratory sheep and goats. The present investigation also confirms the detection of D. nodosus from cases of footrot for the first time from HP. With new reports identifying F. necrophorum as a pathogen with a potential role in aggravating infection caused by footrot, the development of a combined vaccine to prevent lameness in sheep and goats in the north-western Himalayan region has been suggested.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 94%

Infectious lameness among migratory sheep and goats in north-west India, with particular focus on anaerobes

Wani¹,

Verma²,

Sharma³

et al. 2015

Rev. Sci. Tech. OIE

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“…The serogroup specific DNA procured from Sher-e-Kashmir University of Agricultural Sciences and Technology University, Srinagar, India was used as positive control. The serogroup M specific primer was not included in the study as the serogroup was not reported to be prevalent in the state [ 10 ]. The DNA was extracted by boiling method as described earlier by picking up 3-4 individual colonies from each isolate.…”

Section: Methodsmentioning

confidence: 99%

“…Similarly, outbreaks of footrot being reported regularly every year in Andhra Pradesh causing losses to the farming community. Prevalence of serogroups A, B, C, E, F and I were reported in Andhra Pradesh [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of Dichelobacter nodosus serogroup-specific fimA gene from ovine footrot in Andhra Pradesh

et al. 2015

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Aim:Identification of different serogroups of Dichelobacter nodosus prevailing in the region and to understand the degree of genetic heterogeneities among the different isolates of D. nodosus.Materials and Methods:A total of 150 exudate samples of footrot lesions with a lesion score of 2-4 were collected from naturally infected sheep. The samples were screened by polymerase chain reaction (PCR) targeting D. nodosus specific 16srRNA. Of 150 samples screened, 70 samples were found to be positive. The positive samples were attempted for isolation of D. nodosus, out of which 16 isolates were recovered. All the isolates were subjected to serogrouping by multiplex PCR targeting fimA gene using A-I serogroup specific primers.Results:Of 16 isolates, 7 (43.75%) isolates were serogroup B, 4 (25.00%) isolates were serogroup A, 3 isolates (18.75%) were serogroup I and 2 (12.5%) isolates yielded both serogroup A and B. phylogenetic analysis was performed using neighbor-joining algorithm of the ClustelX2 software in order to study whether the serogroups isolated in the present investigation differed genetically from other published serogroups. The fimA gene sequence of present isolates of serogroups A, B, and I were segregated into three distinct groups with high bootstrap values. The serogroup B clustered with Australian isolate of serotype B1 suggesting high genetic similarity of the present isolate with serotype B1.Conclusions:The clinical samples were collected from suspected outbreaks of footrot and identified the prevalence of D. nodosus by PCR targeting 16srRNA gene. Identified serogroups A, B, and I from different districts of Andhra Pradesh. The phylogenetic analysis will help for the tentative identification of serotypes present in the serogroup and to understand the degree of genetic heterogeneities among the different isolates of D. nodosus.

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“…Currently, identification of the D. nodosus and F. necrophorum is being carried out by polymerase chain reaction (PCR) targeting the 16S rRNA [4] and leukotoxin (lktA) genes [10], respectively, without the need to culture the organisms. In India footrot is endemic in the states of Jammu and Kashmir, Andhra pradesh, Tamilnadu, Utter Pradesh etc [3] and [6] and recently its severity increased and attained significant importance in sheep husbandry practices. Thus the aim of the present investigation was to determine the association between the F. necrophorum and D. nodosus in ovine footrot in Kashmir valley and to identify the role of F. necrophorum in the pathogenesis of ovine footrot.…”

Section: Introductionmentioning

confidence: 99%

The detection of Dichelobacter nodosus and Fusobacterium necrophorum from ovine footrot in Kashmir, India

Farooq

Wani

Hassan³

et al. 2015

Anaerobe

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Prevalence of ovine footrot in the tropical climate of southern India and isolation and characterisation of Dichelobacter nodosus

Cited by 18 publications

References 16 publications

Infectious lameness among migratory sheep and goats in north-west India, with particular focus on anaerobes

Infectious lameness among migratory sheep and goats in north-west India, with particular focus on anaerobes

Phylogenetic analysis of Dichelobacter nodosus serogroup-specific fimA gene from ovine footrot in Andhra Pradesh

The detection of Dichelobacter nodosus and Fusobacterium necrophorum from ovine footrot in Kashmir, India

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