Prevalence of osteoporosis and its associated factors among postmenopausal women in Kiambu County, Kenya: a household survey

Sitati, Fred Chuma; Gichangi, Peter; Obimbo, Madadi Moses

doi:10.1007/s11657-020-0685-z

Cited by 9 publications

(7 citation statements)

References 27 publications

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“…The mortality of OP is increasing, and affects 34% of women worldwide (1). It has high rates of morbidity and mortality, and has become a global health problem (2). OP is a multi-factorial bone disease that is related to genetic factors, personal factors, gender, age, and lifestyle, including inadequate calcium intake, excessive alcohol, caffeine consumption, and smoking (3,4).…”

Section: Introductionmentioning

confidence: 99%

Psoralidin prevents caffeine-induced damage and abnormal differentiation of bone marrow mesenchymal stem cells via the classical estrogen receptor pathway

Hua

Zou

et al. 2021

Ann Transl Med

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Background: Caffeine is broadly present in tea, coffee, and cocoa, and is commonly consumed. The bone microenvironment might be damaged by excessive caffeine, which has been shown to exert negative effects on human health. In this study, we sought to determine whether excessive caffeine could damage the biological functions of bone marrow mesenchymal stem cells (BMSCs) and induce bone loss in mice, and further investigate effective therapeutic methods.Methods: BMSCs were treated with different concentrations of caffeine (0.01, 0.05, 0.1, 0.5, and 1.0 mM) for 48 h. Cell counting kit-8 (CCK-8) assay, colony formation assay, wound healing assay, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis were performed to detect the cell viability, proliferation, migration, and pluripotency of BMSCs, respectively. Alizarin red S (ARS) staining, alkaline phosphatase (ALP) staining, oil red O (ORO) staining, and qRT-PCR assay were applied to assess the osteogenic and adipogenic differentiation of BMSCs. BMSCs were treated with caffeine and further exposed to different concentrations of psoralidin (PL) (0.01, 0.1, 1, and 10 μM) for 48 h. Microcomputed tomography (μCT) scanning was used to evaluate the bone mass of mice. 7α-(7-((4,4,5,5,5-Pentafluoropentyl)-sulfiny)nonyl)estra-1,3,5(10)-triene-3,17β-diol (ICI 182,780, ICI) was applied to examine whether the classical estrogen receptor (ER) pathway was involved.Results: The CCK-8 assay, colony formation assay, wound healing assay, and qRT-PCR analysis indicated that caffeine (0.01, 0.05, 0.1, 0.5, 1.0 mM) attenuated the cell viability, proliferation, migration and pluripotency of BMSCs, respectively, in a concentration-dependent manner. Caffeine treatment inhibited osteogenic differentiation but promoted adipogenic differentiation of BMSCs in a dose-dependent manner. Furthermore, ARS staining, ALP staining, ORO staining, and qRT-PCR assay showed that excessive caffeine induced bone loss and osteoporosis (OP) in mice by regulating the osteogenesis and adipogenesis of BMSCs. Also, PL treatment could reverse the caffeine-induced dysfunctions and aberrant differentiation of BMSCs via the ER pathway.

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Section: Introductionmentioning

confidence: 99%

Psoralidin prevents caffeine-induced damage and abnormal differentiation of bone marrow mesenchymal stem cells via the classical estrogen receptor pathway

Hua

Zou

et al. 2021

Ann Transl Med

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“…[ 2 ] Unfortunately, the prevalence of osteoporosis has been rapidly rising worldwide. [ 3 ] Postmenopausal osteoporosis (PMOP) is an estrogen deficiency‐induced osteoporosis, [ 4 ] which is the most common type of osteoporosis. The deficiency of estrogen leads to an increase of bone turnover on all types of bone cells.…”

Section: Introductionmentioning

confidence: 99%

Coenzyme Q10 inhibits RANKL‐induced osteoclastogenesis by regulation of mitochondrial apoptosis and oxidative stress in RAW264.7 cells

Zheng

Cui

Shao

et al. 2021

J Biochem & Molecular Tox

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Coenzyme Q10 (CoQ10) has been reported to improve bone density and the number of trabeculae in postmenopausal osteoporosis, but the mechanism remains to be elucidated. We aimed to investigate the effects of CoQ10 on receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis and the underlying molecular mechanisms. RAW264.7 cells were treated with different concentrations of RANKL to differentiate into osteoclasts, and then these cells were treated with different concentrations of CoQ10 with or without H 2 O 2 . Tartrate-resistant acid phosphatase staining was performed to detect osteoclasts. Cell viability was tested by 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell apoptosis was examined by flow cytometry, and the effects of CoQ10 on protein and messenger RNA expression of mitochondrial apoptosis-associated proteins and osteoclast marker proteins were measured by quantitative reverse transcription polymerase chain reaction and western blot, respectively. Furthermore, enzyme-linked immunosorbent assay was conducted to analyze the activities of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT). RANKL significantly induced osteoclastogenesis in RAW264.7 cells, with the greatest efficiency at 50 ng/ml. CoQ10 had no significant effects on cell viability but it significantly increased the percentages of cell apoptosis.Mechanically, CoQ10 statistically decreased the levels of Bcl-2 and cytochrome C in mitochondria and upregulated the levels of Bax, cleaved caspase 3, and cytochrome C in the cytoplasm. Moreover, CoQ10 significantly decreased RANKL-induced osteoclastogenesis regardless of exposure to H 2 O 2 . In addition, CoQ10 statistically reduced MDA activity and elevated the activities of SOD and CAT, as well as the expression of oxidative stress-related proteins. CoQ10 may inhibit RANKL-induced osteoclastogenesis by regulation of mitochondrial apoptosis and oxidative stress in RAW264.7 cells.

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“…É necessário observar que a população avaliada neste estudo é bastante particular, seguramente composta por idosos frágeis ou em fragilização, posto que foram assistidos em uma unidade de referência. Os estudos nacionais (Costa et al, 2016;Rodrigues & Barros, 2016;Silva et al, 2015;Sitati et al, 2020), que investigam prevalência da OP são bastante heterogêneos em seus aspectos metodológicos e, consequentemente, em relação aos resultados.…”

Section: Discussionunclassified

“…Estudo por inquerido telefônico realizado no Brasil com 54.369 indivíduos (21.294 homens e 33.075 mulheres) a partir dos 18 anos, observou-se maior prevalência de OP (autorreferida) entre aqueles com idade igual ou superior a 65 anos e do sexo feminino (Silva et al, 2015). Considerando apenas mulheres submetidas ao exame de DO, um trabalho realizado na região Sul do Brasil identificou prevalência de 25.4% de OP para aquelas com mais de 66 anos (Sitati et al, 2020) (maior que 27) é um fator de proteção. Os resultados observados são compatíveis com a literatura, que registra que as mulheres apresentam maior susceptibilidade à OP, havendo perda óssea acelerada nos anos posteriores à menopausa (Alibasic et al, 2020;Bailey et al, 2019;Kalkim & Daghan, 2017;Sitati et al, 2020;Skrzek et al, 2014).…”

Section: Discussionunclassified

Fatores Associados À Osteoporose Em Pessoas Idosas Em Um Centro De Referência

de Figueiredo Braga Colares,

Bromenschenkel Vasconcelos,

Colares Maia

et al. 2024

PSYCHTECH

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Este estudo objetivou estimar a prevalência de osteoporose em idosos atendidos em um centro de referência e identificar fatores associados. Trata-se de um estudo transversal, analítico, com coleta de dados em prontuários médicos e entrevistas aos idosos atendidos no Centro de Referência em Assistência à Saúde dos Idosos (CRASI), e que realizaram exame de densitometria óssea nos anos de 2017 e 2018. Utilizou-se análise bivariada para investigar a associação entre osteoporose e características do grupo: perfil sociodemográfico, Índice de Massa Corporal (IMC), hábitos de vida, cuidados e condições de saúde. As variáveis com associação até o nível de 20% (p ≤ .20) foram avaliadas de forma conjunta por regressão múltipla de Poisson com estimador robusto, admitindo-se para o modelo final apenas as variáveis com nível de significância de até 5%. A prevalência de osteoporose foi de 48.5%. No modelo final ficaram associadas as variáveis: sexo feminino (RP = 1.88; IC95%: 1.36 - 2.60), idade acima de 80 anos (RP = 1.55; IC95%: 1.29 - 1.85), IMC menor que 22 (RP = 1.23; IC95%: 1.02 - 1.49) e como protetor IMC acima de 27 (RP = .52; IC95%: .41 - .67). Concluindo,verificou-se alta prevalência de osteoporose e associação como fator modificável o baixo peso e outros não modificáveis: sexo e idade. Não foram identificadas associações com variáveis relacionadas aos hábitos de vida.

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Prevalence of osteoporosis and its associated factors among postmenopausal women in Kiambu County, Kenya: a household survey

Cited by 9 publications

References 27 publications

Psoralidin prevents caffeine-induced damage and abnormal differentiation of bone marrow mesenchymal stem cells via the classical estrogen receptor pathway

Psoralidin prevents caffeine-induced damage and abnormal differentiation of bone marrow mesenchymal stem cells via the classical estrogen receptor pathway

Coenzyme Q10 inhibits RANKL‐induced osteoclastogenesis by regulation of mitochondrial apoptosis and oxidative stress in RAW264.7 cells

Fatores Associados À Osteoporose Em Pessoas Idosas Em Um Centro De Referência

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