Prevalence of nonalcoholic fatty liver disease (NAFLD) in patients of cardiovascular diseases and its association with hs-CRP and TNF-α

Ajmal, Masihur Rehman; Yaccha, Monika; Malik, Mohammad Azharuddin; Rabbani, M U; Ahmad, Irshad; Isalm, Najmul; Abdali, Nasar

doi:10.1016/j.ihj.2014.08.006

Cited by 54 publications

(41 citation statements)

References 26 publications

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“…Although the remaining indirect effects of elevated liver fat are unmeasured in this study, the link between NAFLD and low-grade inflammation 18 makes it likely that this also underpins some of the observed relationship between liver fat and aortic stiffness. In line with this, C-reactive protein, interleukin-6 and tumor necrosis factor-α levels have all been found to correlate with both NAFLD and aortic stiffness.…”

Section: Hepatic Fat Content Inflammation and Aortic Stiffnessmentioning

confidence: 87%

Investigating a Liver Fat

Rider

Banerjee

Rayner

et al. 2016

ATVB

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Circulating triglyceride levels are dictated not only by dietary intake but also by hepatic triglyceride production, which is elevated in NAFLD. This suggests that NAFLD may be exerting its negative effects on vascular function indirectly via increasing blood triglyceride levels. As a result, we hypothesized that the elevated liver fat levels in obesity would be linked to aortic stiffness, and that effect would, at least in part, be mediated indirectly via increasing serum triglyceride levels. Should © 2015 American Heart Association, Inc. Objective-To investigate the relationship between hepatic fat content, circulating triglyceride levels and aortic stiffness in adult and childhood obesity. Approach and Results-Seventy-seven adults and 18 children across a wide range of body mass index (18.5-52.6 kg/ m 2; percentile 8-100) with no identifiable cardiac risk factors underwent; 1H-magnetic resonance spectroscopy to quantify hepatic fat content and magnetic resonance imaging to assess aortic pulse wave velocity (PWV) and regional distensibility. In adults, multivariable regression showed age (β=0.09; P=0.02), liver fat (β=2.5; P=0.04), and serum triglyceride (β=0.47; P=0.01) to be independent predictors of PWV. Age and blood pressure-adjusted, moderated regression showed that 43% of the total negative effect of hepatic fat on PWV is attributable to indirect effects via increased triglyceride (P=0.005). In addition, regional distensibility was positively correlated with hepatic fat (ascending; r=−0.35; descending, r=−0.23; abdominal, r=−0.41; all P<0.001). Similar to that seen in adults, PWV (r=0.72; P<0.001) and abdominal regional distensibility (r=−0.52; P<0.001) were correlated with liver fat in children. Conclusions-Increasing age, liver fat, and triglyceride are all related to increased aortic stiffness in adults. Even when controlling for the effects of age and blood pressure, hepatic fat has a negative effect on PWV, with substantial indirect effect occurring via increased circulating triglyceride level. This relationship between hepatic fat and aortic stiffness occurs early in the obesity process and is also seen in children. As such, hepatic fat content is a potential therapeutic target to treat the elevated vascular risk in obesity. regional aortic distensibility (AD) 14 and abdominal visceral fat, 1H magnetic resonance spectroscopy assessment of liver fat content, dual-energy x-ray absorptiometry assessment of total fat mass, and fasting blood tests for serum triglyceride levels. Materials and MethodsMaterials and Methods are available in the online-only Data Supplement. ResultsA summary of the anthropometric data for the adult and childhood study groups is shown in Table 1. Table 3). Adult Cohort Hepatic Fat Content in AdultsStepwise multivariable regression of these variables revealed showed triglyceride (β=3.6; P=0.009) and liver fat (β=2.5; P=0.008) to be independent predictors of increased PWV in this model (overall R 2 of the model 0.42; P<0.001; Table 4). To further explore the relationship be...

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Section: Hepatic Fat Content Inflammation and Aortic Stiffnessmentioning

confidence: 87%

Investigating a Liver Fat

Rider

Banerjee

Rayner

et al. 2016

ATVB

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“…In contrast, we did not find any correlations between adhesive molecules and BMI or TNF-a and BMI. Strong correlations have been found between central adiposity and adhesive molecules ICAM-1 and VCAM-1 in obese subjects 40 and TNF-a with nonalcoholic fatty liver disease (NAFLD), 41 reinforcing the potential link between visceral adiposity and increased risk for heart disease. The lack of correlations between these molecules and BMI suggests that central obesity and NAFLD play a key role in the circulating pool of these inflammatory markers independent of BMI.…”

Section: Plasma Lipids and Lipoproteinsmentioning

confidence: 92%

A Larger Body Mass Index is Associated with Increased Atherogenic Dyslipidemia, Insulin Resistance, and Low-Grade Inflammation in Individuals with Metabolic Syndrome

Ebron

Andersen

Aguilar

et al. 2015

Metabolic Syndrome and Related Disorders

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“…Cell Culture-The NCTC1469 murine liver cell line (American Type Culture Collection) was cultured in low-glucose Dulbecco's modified Eagle's medium (L-DMEM) (Invitrogen) supplemented with 10% horse serum (HyClone), 100 units/ml penicillin (Invitrogen), and 0.1 mg/ml streptomycin (HyClone) at 37°C with humidified air and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…It is the most prevalent chronic liver disease worldwide and is strongly associated with various metabolic disorders, such as obesity, type 2 diabetes, and cardiovascular disease (1)(2)(3). The disease may progress to nonalcoholic steatohepatitis (NASH), hepatic fibrosis/cirrhosis, and hepatocellular carcinoma due to the co-existence of other detrimental factors (4,5).…”

mentioning

confidence: 99%

Liver MicroRNA-291b-3p Promotes Hepatic Lipogenesis through Negative Regulation of Adenosine 5′-Monophosphate (AMP)-activated Protein Kinase α1

Meng

Guo

Fang

et al. 2016

Journal of Biological Chemistry

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In a microarray study, we found that hepatic miR-291b-3p was significantly increased in leptin-receptor-deficient type 2 mice (db/db), a mouse model of diabetes. The function of miR291b-3p is unknown. The potential role of miR-291b-3p in regulating hepatic lipid metabolism was explored in this study. High-fat diet (HFD)-and chow-fed mice were injected with an adenovirus expressing a miR-291b-3p inhibitor and a miR291b-3p mimic through the tail vein. Hepatic lipids and lipogenic gene expression were analyzed. Additionally, gain-and loss-of-function studies were performed in vitro to identify direct targets of miR-291b-3p. MiR-291b-3p expression and the protein levels of sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS) were increased in the steatotic liver of db/db mice and HFD-fed mice versus their respective controls. Inhibition of hepatic miR-291b-3p expression prevented increases in hepatic lipogenesis and steatosis in HFD-fed mice. The opposite was observed when miR-291b-3p was overexpressed in the livers of chow-fed C57BL/6J wild-type mice. In vitro studies revealed that silencing of miR-291b-3p in NCTC1469 hepatic cells ameliorated oleic acid/palmitic acid mixture-induced elevation of cellular triglycerides. Importantly, we identified AMP-activated protein kinase (AMPK)-␣1 as a direct target of miR-291b-3p. Using metformin, an activator of AMPK, we showed that AMPK activation-induced inhibition of hepatic lipid accumulation was accompanied by reduced expression of miR-291b-3p in the liver. Liver miR-291b-3p promoted hepatic lipogenesis and lipid accumulation in mice. AMPK␣1 is a direct target of miR-291b-3p. In conclusion, our findings indicate that miR-291b-3p promotes hepatic lipogenesis by suppressing AMPK␣1 expression and activity, indicating the therapeutic potential of miR-291b-3p inhibitors in fatty liver disease.

show abstract

Prevalence of nonalcoholic fatty liver disease (NAFLD) in patients of cardiovascular diseases and its association with hs-CRP and TNF-α

Cited by 54 publications

References 26 publications

Investigating a Liver Fat

Investigating a Liver Fat

A Larger Body Mass Index is Associated with Increased Atherogenic Dyslipidemia, Insulin Resistance, and Low-Grade Inflammation in Individuals with Metabolic Syndrome

Liver MicroRNA-291b-3p Promotes Hepatic Lipogenesis through Negative Regulation of Adenosine 5′-Monophosphate (AMP)-activated Protein Kinase α1

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