Prevalence of Mycobacterium tuberculosis Beijing genotype and its association with drug resistance in North India

Mathuria, Jitendra Prasad; Srivastava, Govind; Sharma, Pragya; Mathuria, Bharat Lal; Ojha, Sanjay Kumar; Katoch, Vishwa Mohan; Anupurba, Shampa

doi:10.1016/j.jiph.2016.06.007

Cited by 16 publications

(8 citation statements)

References 21 publications

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“…A systematic review and meta-analysis on the prevalence of the Beijing genotype strain in world population revealed that its prevalence was 44.7% in Asia, 27.9% in Europe, 12.5% in Africa, and 8.9% in America [22]. In India, the Beijing genotype strain is present in a low percentage throughout the country including a previous study in our region [23][24][25] however we observed a comparatively higher incident rate of the Beijing genotype strain (19.95%, 76/ 381). Possible reason behind this discrepancy could be the low number of MTB strains studied in the previous study in our region.…”

Section: Discussionmentioning

confidence: 81%

Detection of Beijing strains of MDR M. tuberculosis and their association with drug resistance mutations in katG, rpoB, and embB genes

et al. 2020

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Background Molecular epidemiological studies of Mycobacterium tuberculosis (MTB) are the core of current research to find out the association of the M. tuberculosis genotypes with its outbreak and transmission. The high prevalence of the Beijing genotype strain among multidrug resistance (MDR) TB has already been reported in various studies around India. The overall objective of this study was to detect the prevalence of Beijing genotype strains of MDR M. tuberculosis and their association with the clinical characteristics of TB patients. Methods In this study 381 M. tuberculosis clinical isolates were obtained from sputum samples from 2008 to 2014. The multiplex-PCR and Spoligotyping (n = 131) methods were used to investigate the prevalence of the Beijing genotype strain by targeting the Rv2820 gene and their association with drug resistance and clinical characteristics of TB patients. The drug susceptibility testing of first-line anti-TB drugs was performed by using the proportion method and MGIT960. A collection of isolates having Beijing and non-Beijing strains were also characterized to see if Beijing genotype strains had a higher rate of mutations at codons 516, 526 and 531 of the 81-bp region of the rpoB gene, codon 315 of the katG gene, and codon 306 of the embB gene. Results The sensitivities and specificities of multiplex-PCR assay compared to that of standard Spoligotyping was detected to be 100%. Further, we observe that the multi drug-resistance was significantly associated with Beijing genotype strains (p = 0.03) and a strong correlation between Beijing genotype strains and specific resistance mutations at the katG315, rpoB531, and embB306 codons (p = < 0.0001, < 0.0001 & 0.0014 respectively) was also found. Conclusions This rapid, simple, and cost-effective multiplex PCR assay can effectively be used for monitoring the prevalence of Beijing genotype strains in low resource settings. Findings of this study may provide a scientific basis for the development of new diagnostic tools for detection and effective management of DR-TB in countries with a higher incidence rate of Beijing genotype strains.

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Section: Discussionmentioning

confidence: 81%

Detection of Beijing strains of MDR M. tuberculosis and their association with drug resistance mutations in katG, rpoB, and embB genes

et al. 2020

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“…The Beijing genotype comprises over 50% of the tuberculosis species in East Asia and 13% of global isolates [27]. Beijing family isolates have emerged as endemic and predominant genotypes in East and South Asia, South Africa and countries of the former Soviet Union [28]. Four ecological patterns for the Beijing genotype have been reported: epidemic and associated with drug resistance; endemic and not associated with drug resistance; epidemic but susceptible to drug; at a very low level or absent [29].…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium tuberculosis Beijing genotype strains and unfavourable treatment outcomes: a systematic review and meta-analysis

Wang

Martínez

et al. 2020

Clinical Microbiology and Infection

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Objectives: The Mycobacterium tuberculosis Beijing genotype was first described in 1995 and is now the predominant strain among patients with tuberculosis in many Asian countries. The rapid global spread of the Beijing genotype is receiving increasing attention because it can cause a higher risk of treatment failures. Our objective was to assess the association between the Beijing genotype and unfavourable treatment outcomes of tuberculosis. Methods: We searched for eligible studies through PubMed, Web of Science, Chinese National Knowledge Infrastructure and Wanfang Data. We included cohort studies that evaluated treatment outcomes and Beijing genotype strains. Participants were individuals with active pulmonary tuberculosis. The association between Beijing genotype and the risk of unfavourable treatment outcomes was assessed using the pooled odds ratios (ORs) with corresponding confidence intervals (CIs). Results: In total, 7489 tuberculosis patients were involved in the analysis. Patients infected with the Beijing genotype were more likely to have unfavourable treatment outcomes, with the OR of 2.04 (95% CI 1.52e2.75). The pooled OR was 2.33 (95% CI 1.71e3.16) for recurrence, 2.36 (95% CI 1.69e3.30) for relapse and 2.62 (95% CI 1.90e3.61) for treatment failure, respectively. Subgroup analysis revealed that Beijing genotype was a significant risk factor for unfavourable treatment outcomes in Asians (OR 2.28, 95% CI 1.82e2.86) or in drug-susceptible TB patients (OR 2.11, 95% CI 1.31e3.39). No significant association was observed among non-Asian populations (OR 1.17, 95% CI 0.73e1.86) or patients with multidrug-resistant (MDR) tuberculosis (OR 0.97, 95% CI 0.48e1.94). Conclusions: Our results suggest that Mycobacterium tuberculosis Beijing genotype is associated with an increased risk of unfavourable treatment outcomes, including treatment failure and relapse.

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“…However, Barrón and colleagues, in an animal interventional study, showed that Beijing strain increases virulence after exposure to BCG‐induced immunity in vaccinated hosts (interaction between Mtb Beijing‐genotype strains and BCG‐vaccinated hosts) [23]. In other words, there is concern that Beijing strains may have a tendency for emerging drug resistance and may be spreading worldwide, probably as a result of increased virulence [24–29]. In some parts of the world; for example, in Russia, Estonia and Azerbaijan, a very strong relationship between Beijing families and TB drug resistance was reported [19,24–28].…”

Section: Discussionmentioning

confidence: 99%

“…In other words, there is concern that Beijing strains may have a tendency for emerging drug resistance and may be spreading worldwide, probably as a result of increased virulence [24–29]. In some parts of the world; for example, in Russia, Estonia and Azerbaijan, a very strong relationship between Beijing families and TB drug resistance was reported [19,24–28]. In some countries where the Beijing strain prevalence is low or absent, the high protective efficacy of the vaccine has been maintained and it seems that these strains have defied almost all measures to conflict TB [18].…”

Section: Discussionmentioning

confidence: 99%

Does the BCG vaccine have different effects on strains of tuberculosis?

Kazemi

Farajnia

Ansarin

et al. 2020

Clinical and Experimental Immunology

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Summary Several explanations have been suggested concerning the variety in bacille Calmette–Guerin (BCG) vaccine efficacy on strains of Mycobacterium tuberculosis (Mtb). This study aimed to compare the effect of BCG vaccination history in the prevention of the occurrence of Mtb-Beijing and non-Beijing strains. In this cross-sectional study, 64 patients with pulmonary tuberculosis (TB) were recruited from the Iranian border provinces (North West and West). Isolates were subjected to restriction fragment length polymorphism (RFLP) analysis, using the insertion sequence IS6110 as a probe (IS6110 RFLP) and drug susceptibility testing using the proportion method. Samples were analyzed with Gel Compare II 6.6 and spss version 18. The mean age [standard deviation (SD)] of the patients was 54·4 (SD = 17·0). Overall, 49 cases (76·56%) had no BCG vaccination scar. The prevalence of Beijing strains was 9·38% and drug resistance proportion among the isolates was 14·1% (nine cases). There was a significant relationship between Beijing strains and tuberculosis (TB)-drug resistance in isolates (χ2 = 26·29, P < 0·001). There was also a strong association between vaccination history and Beijing strains (χ2 = 13·23, P = 0·002). Also, a statistical relationship was observed between Beijing strains and drug-resistant TB among patients with a history of vaccination (χ2 = 7·47, P = 0·002). This association was not maintained in the unvaccinated group (P = 0·102). These findings confirm the claim that the vaccine has different effects on different subspecies of tuberculosis. The cause of the high probability of drug resistance in patients with Beijing-TB and vaccination history requires further investigation with a higher sample size.

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Prevalence of Mycobacterium tuberculosis Beijing genotype and its association with drug resistance in North India

Cited by 16 publications

References 21 publications

Detection of Beijing strains of MDR M. tuberculosis and their association with drug resistance mutations in katG, rpoB, and embB genes

Detection of Beijing strains of MDR M. tuberculosis and their association with drug resistance mutations in katG, rpoB, and embB genes

Mycobacterium tuberculosis Beijing genotype strains and unfavourable treatment outcomes: a systematic review and meta-analysis

Does the BCG vaccine have different effects on strains of tuberculosis?

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