Staphylococcus aureus expresses a variety of extracellular proteins and polysaccharides, some of which are correlated with virulence. The aims of study were to identify of multidrug-resistant S. aureus(MDRSA) strains isolated from different sites of patients, to determine their pathogenicity factors, enzymes, ability of carbohydrate fermentation. Study results show that some determinants of pathogenicity (plasma coagulase, catalase, urease, lecithinase production, hemolysis, proteolysis), also carbohydrates and mannitol fermentation in aerobic and anaerobic conditions arecharacterized high activity in MDRSA strains. The most common wasisolation of these strains from patients with oral cavity infections, a few cases -from peritoneal liquid and phlegmon.