Prevalence of methicillin resistance and virulence determinants of &amp;lt;i&amp;gt;Staphylococcus aureus&amp;lt;/i&amp;gt; in diabetic foot ulcers

Sandhu, Sumeet; Rathnayake, Irani; Huygens, Flavia

doi:10.5455/2319-2003.ijbcp20141201

Cited by 2 publications

(2 citation statements)

References 17 publications

(23 reference statements)

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“…In contrast, using a swab to obtain material from the surface of a wound is not invasive and does not involve any of the above sampling technique disadvantages. A number of research groups have routinely utilized wound swab samples for DNA sequencing studies in preference to tissue biopsies or curettage [4,13,14]. The limitations of collecting wound swabs has been investigated using cultivation-based studies which have reported low concordance of isolated bacteria when compared to tissue specimens [15,16].…”

Section: Introductionmentioning

confidence: 99%

The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

Travis

Malone

et al. 2020

BMC Microbiol

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Background: Health-care professionals need to collect wound samples to identify potential pathogens that contribute to wound infection. Obtaining appropriate samples from diabetic foot ulcers (DFUs) where there is a suspicion of infection is of high importance. Paired swabs and tissue biopsies were collected from DFUs and both sampling techniques were compared using 16S rRNA gene sequencing. Results: Mean bacterial abundance determined using quantitative polymerase chain reaction (qPCR) was significantly lower in tissue biopsies (p = 0.03). The mean number of reads across all samples was significantly higher in wound swabs ðX = 32,014) compared to tissue (X = 15,256, p = 0.001). Tissue biopsies exhibited greater overall diversity of bacteria relative to swabs (Shannon's H diversity p = 0.009). However, based on a presence/ absence analysis of all paired samples, the frequency of occurrence of bacteria from genera of known and potential pathogens was generally higher in wound swabs than tissue biopsies. Multivariate analysis identified significantly different bacterial communities in swabs compared to tissue (p = 0.001). There was minimal correlation between paired wound swabs and tissue biopsies in the number and types of microorganisms. RELATE analysis revealed low concordance between paired DFU swab and tissue biopsy samples (Rho = 0.043, p = 0.34). Conclusions: Using 16S rRNA gene sequencing this study identifies the potential for using less invasive swabs to recover high relative abundances of known and potential pathogen genera from DFUs when compared to the gold standard collection method of tissue biopsy.

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Section: Introductionmentioning

confidence: 99%

The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

Travis

Malone

et al. 2020

BMC Microbiol

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show abstract

“…They were able to detect 44% S. aureus from the study population. They also targeted the mecA gene to detect MRSA and found 25% of the samples to be positive for MRSA [43].…”

Section: Detection Techniquesmentioning

confidence: 99%

Bacterial Diversity with Emerging Antimicrobial Resistance of Diabetic Foot Ulceration and Current Detection Techniques: A Review

Alvi

2021

ELECTRON J GEN MED

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Prevalence of methicillin resistance and virulence determinants of <i>Staphylococcus aureus</i> in diabetic foot ulcers

Cited by 2 publications

References 17 publications

The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

The microbiome of diabetic foot ulcers: a comparison of swab and tissue biopsy wound sampling techniques using 16S rRNA gene sequencing

Bacterial Diversity with Emerging Antimicrobial Resistance of Diabetic Foot Ulceration and Current Detection Techniques: A Review

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