Prevalence of Met-203 type spaA variant in Erysipelothrix rhusiopathiae isolates and the efficacy of swine erysipelas vaccines in Japan

Uchiyama, Mitsuru; Yamamoto, Kazuhiro; Ochiai, Mariko; Yamamoto, Tsukasa; Hirano, Fumiya; Imamura, Saiki; Nagai, Hidetaka; Ohishi, Kazuhito; Horiuchi, Noriyuki; Kijima, Mayumi

doi:10.1016/j.biologicals.2013.12.002

Cited by 20 publications

(35 citation statements)

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“…This study represents the largest examination of the diversity of SpaA protein sequences in E. rhusiopathiae conducted to date, based on a collection of publicly available sequences and de novo whole genome sequence assemblies (n = 343). With this extensive collection of sequences, we were able to identify additional amino acid variants that could further discriminate SpaA protein sequences in comparison to previous typing schemes (Uchiyama et al, 2014;Janßen et al, 2015). We were also able to assess for correlation of these groups with population structure (Figure 5).…”

Section: Spaa Diversity Correlation With Population Structure and Rmentioning

confidence: 99%

“…SpaA also plays an important role in pathogenesis by increasing resistance to phagocytosis and promoting endothelial adherence (Harada et al, 2014;Borrathybay et al, 2015;Zhu et al, 2017a,b). Several variants (groups) of the SpaA protein have already been described, based on amino acid differences within the immunoprotective domain (Uchiyama et al, 2014;Janßen et al, 2015;Ogawa et al, 2017). While tests have been conducted on both mice and pigs to assess cross-protection among different Spa types using strains representing multiple serotypes , it remains unclear whether amino acid variants within the SpaA protein can result in differences in protection.…”

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confidence: 99%

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Genomic and Immunogenic Protein Diversity of Erysipelothrix rhusiopathiae Isolated From Pigs in Great Britain: Implications for Vaccine Protection

et al. 2020

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Frontiers in Microbiology | www.frontiersin.org March 2020 | Volume 11 | Article 418 Forde et al. Genomic Diversity of Erysipelothrix rhusiopathiaecommonplace for this bacterium. We hypothesize that the sequence variants in these proteins could be responsible for differences in the efficacy of the immune response. Our results provide the necessary basis for testing this hypothesis through in vitro and in vivo studies.

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Section: Spaa Diversity Correlation With Population Structure and Rmentioning

confidence: 99%

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confidence: 99%

Genomic and Immunogenic Protein Diversity of Erysipelothrix rhusiopathiae Isolated From Pigs in Great Britain: Implications for Vaccine Protection

et al. 2020

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“…Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals and erysipeloid in humans . In Japan, the incidence of acute swine erysipelas has been increasing since 2008 . It has been reported that increased cases of acute swine erysipelas are associated with clonal strains belonging to a certain lineage of E. rhusiopathiae ; however, the reason for the increase in cases has been unclear, and it has not been determined whether these clonal strains are prevalent among only domesticated pigs or are widespread in wild animals.…”

Section: Prevalence (Positive Rate) Of Antibodies Against Erysipelothmentioning

confidence: 99%

Wild boars: A potential source of Erysipelothrix rhusiopathiae infection in Japan

Shimoji

Osaki

Ogawa

et al. 2019

Microbiology and Immunology

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The potential role of wild boars as a source of erysipelas infection was investigated. An ELISA test of wild boar serum samples from 41 prefectures in Japan revealed that proportions of the Erysipelothrix rhusiopathiae‐positive samples were very high in all the prefectures, and the mean positive rate was 95.6% (1312/1372). Serovars of E. rhusiopathiae isolates from wild boars were similar to those of previously reported swine isolates, and all serovar isolates tested were found to be pathogenic to mice. These results suggest that wild boars in Japan constitute a reservoir of E. rhusiopathiae and may pose risks to other animals.

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“…Based on the sequences in this region, serovar 1a clinical isolates possessing SpaA with a methionine at position 203 (M203-SpaA), in which the third position in the codon is guanine (G) at nucleotide position 609 of the gene, have been reported to be widespread in pigs in Japan (13,14).…”

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confidence: 99%

Clonal Lineages of Erysipelothrix rhusiopathiae Responsible for Acute Swine Erysipelas in Japan Identified by Using Genome-Wide Single-Nucleotide Polymorphism Analysis

Ogawa

Shiraiwa

Ogura

et al. 2017

Appl Environ Microbiol

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Erysipelothrix rhusiopathiae causes swine erysipelas, an important infectious disease in the swine industry. In Japan, the incidence of acute swine erysipelas due to E. rhusiopathiae serovar 1a has recently increased markedly. To study the genetic relatedness of the strains from the recent cases, we analyzed 34 E. rhusiopathiae serovar 1a swine isolates collected between 1990 and 2011 and further investigated the possible association of the live Koganei 65-0.15 vaccine strain (serovar 1a) with the increase in cases. Pulsed-field gel electrophoresis analysis revealed no marked variation among the isolates; however, sequencing analysis of a hypervariable region in the surface-protective antigen A gene (spaA) revealed that the strains isolated after 2007 exhibited the same spaA genotype and could be differentiated from older strains. Phylogenetic analysis based on genome-wide single-nucleotide polymorphisms (SNPs) revealed that the Japanese strains examined were closely related, showing a relatively small number of SNPs among them. The strains were classified into four major lineages, with Koganei 65-0.15 (lineage III) being phylogenetically separated from the other three lineages. The strains isolated after 2007 and the two older strains constituted one major lineage (lineage IV) with a specific spaA genotype (M203/I257-SpaA), while the recent isolates were further divided into two geographic groups. The remaining older isolates belonged to either lineage I, with the I203/L257-SpaA type, or lineage II, with the I203/ I257-SpaA type. These results indicate that the recent increased incidence of acute swine erysipelas in Japan is associated with two sublineages of lineage IV, which have independently evolved in two different geographic regions.IMPORTANCE Using large-scale whole-genome sequence data from Erysipelothrix rhusiopathiae isolates from a wide range of hosts and geographic origins, a recent study clarified the existence of three distinct clades (clades 1, 2, and 3) that are found across multiple continents and host species, representing both livestock and wildlife, and an "intermediate" clade between clade 2 and the dominant clade 3 within the species. In this study, we found that the E. rhusiopathiae Japanese strains examined exhibited remarkably low levels of genetic diversity and confirmed that all of the Japanese and Chinese swine isolates examined in this study belong to clonal lineages within the intermediate clade. We report that spaA genotyping of E. rhusiopathiae strains is a practical alternative to whole-genome sequencing analysis of the E. rhusiopathiae isolates from eastern Asian countries.

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Prevalence of Met-203 type spaA variant in Erysipelothrix rhusiopathiae isolates and the efficacy of swine erysipelas vaccines in Japan

Cited by 20 publications

References 17 publications

Genomic and Immunogenic Protein Diversity of Erysipelothrix rhusiopathiae Isolated From Pigs in Great Britain: Implications for Vaccine Protection

Genomic and Immunogenic Protein Diversity of Erysipelothrix rhusiopathiae Isolated From Pigs in Great Britain: Implications for Vaccine Protection

Wild boars: A potential source of Erysipelothrix rhusiopathiae infection in Japan

Clonal Lineages of Erysipelothrix rhusiopathiae Responsible for Acute Swine Erysipelas in Japan Identified by Using Genome-Wide Single-Nucleotide Polymorphism Analysis

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