Prevalence of maternal HIV infection based on unlinked anonymous testing of newborn babies

Peckham, Catherine; Ades, A E; O’Connor, Clare; Tedder, Richard S.; Briggs, Moya; Parra-Mejia, N; Hjelm, M.; Wilcox, A H

doi:10.1016/0140-6736(90)90746-r

Cited by 69 publications

(20 citation statements)

References 11 publications

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“…This method of blood specimen collection has the advantage that specimens can be collected, stored and transported without prior processing, and minimizes the risk of laboratory acquired infection. The costeffective modified PA assays used in this study for the detection of antibodies to both HTLV-I and HIV-1 have been shown to be highly sensitive, specific and suitable for epidemiological studies [33,38,39].…”

Section: Discussionmentioning

confidence: 99%

“…Elution of serum A 5*5 mm diameter spot was punched out of each DBS and eluted in 100 ,ul elution buffer (phosphatebuffered saline, pH 7-2, containing 0 05 % 'Tween 20' and 0 05 % sodium azide) as described by Peckham and colleagues [38].…”

Section: Dried Blood Spot (Dbs) Collectionmentioning

confidence: 99%

“…The eluates were tested for anti-HIV-1 in a modified gelatin PA assay as described previously [33,38].…”

Section: Assay For the Detection Of Antibodies To Hiv-1mentioning

confidence: 99%

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Seroepidemiology of HTLV-I in relation to that of HIV-1 in the Gauteng region, South Africa, using dried blood spots on filter papers

Taylor

Parker²,

Crewe-Brown

et al. 1996

Epidemiol. Infect.

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SUMMARYThe seroprevalence of human T-lymphotropic virus type I (HTLV-I), in relation to that of human immunodeficiency virus type 1 (HIV-1), was determined in a comparative unlinked anonymous antenatal and neonatal (for indirect measurement of maternal antibodies) serosurvey in the Gauteng region of South Africa, using dried blood spots (DBS) and modified particle agglutination assays. Samples were confirmed to be antibody positive by western blot. A total of 2582 DBS collected during 1993 and 1994 from subjects of African, European and coloured origin were tested. Ten were confirmed as positive for HTLV-I and 128 for HIV-1. No antibodies to HTLV-I or HIV-1 were demonstrated in the 221 women of European and coloured origin who were screened. The HTLV-I seroprevalence rate in subjects of African origin appeared to increase -from 0 % in 1993 to 0-49 % in 1994, while HIV-1 seroprevalence rates of 5-2 % and 5-4 % were recorded for 1993 and 1994 respectively. No significant differences in HTLV-I and HIV-1 seroprevalence rates were noted for the two areas investigated. These results indicate that HTLV-I should be included in infection control programs, and provide baseline data for monitoring the possible spread of HTLV-I in the heterosexual population in this region.

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Section: Discussionmentioning

confidence: 99%

Section: Dried Blood Spot (Dbs) Collectionmentioning

confidence: 99%

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Seroepidemiology of HTLV-I in relation to that of HIV-1 in the Gauteng region, South Africa, using dried blood spots on filter papers

Taylor

Parker²,

Crewe-Brown

et al. 1996

Epidemiol. Infect.

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“…Initially, antibodies are relatively robust and remain unaffected by fluctuations in temperature during transportation and storage; however, humidity and desiccation do play important roles, and long-term storage conditions must be carefully considered (29,30,(35)(36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

Duncombe

Commander

Erdenliğ

et al. 2013

Clin Vaccine Immunol

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c Brucella abortus, a smooth strain of the genus Brucella, is the causative agent of bovine brucellosis. To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid samples and may aid in diagnostic programs and validation studies. To evaluate the utility of these cards, 204 bovine blood serum samples from Brucella-infected and noninfected animals were stored on and eluted from the Protein Saver cards. Anti-Brucella smooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). The results showed a highly significant correlation between titers from the serum eluates and the unprocessed sera. Therefore, under these circumstances, serum eluates and unprocessed serum samples may be used interchangeably. Blood plasma from 113 mitogen-stimulated whole-blood samples was added to and eluted from the Protein Saver cards. The gamma interferon (IFN-␥) titers in the plasma eluates were compared to those of the unprocessed plasma samples obtained by IFN-␥ ELISA. The results showed a significant correlation between the plasma eluates and the unprocessed plasma samples. To derive a signal in the plasma eluate, it was necessary to develop a novel and highly sensitive ELISA for the detection of IFN-␥. The serum samples stored on cards at room temperature over a 10-day period showed little variation in antibody titers. However, the plasma eluates showed a progressive loss of IFN-␥ recovery over 10 days when stored at room temperature.

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“…Other reports describe the successful use of such blood spots in newborns for the detection of maternal HIV [11,12] or HTLV-I [13] antibodies for epidemiological purposes. Also HBs antigen and other markers of HBV infection have already been detected in dried blood spots [14,15].…”

Section: Discussionmentioning

confidence: 99%

Detection of HIV‐1 Infection in Dried Blood Spots from a 12‐Year‐Old ABO Bedside Test Card

Strobel

Emminger

Mayer³

et al. 1998

Vox Sanguinis

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Background and Objectives: We tested dried blood from an ABO bedside test card which had been stored at room temperature for 12 years, to prove that a patient with HIV‐1 infection had been infected by blood transfusion. Materials and Methods: Immunoblots for HIV‐1 antibodies and threefold PCRs with halfnested primers for the HIV‐1 integrase gene were done with eluates from the dried blood spots. Results: HIV‐1 antibodies and HIV‐1 DNA could be detected in the sample from one unit of blood, but not from the two other units or from the recipient before transfusion. Conclusion: Further studies should be done on the validity of stored dried blood as an alternative to the storage of frozen donor serum for several years for ‘look‐back’ studies.

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Prevalence of maternal HIV infection based on unlinked anonymous testing of newborn babies

Cited by 69 publications

References 11 publications

Seroepidemiology of HTLV-I in relation to that of HIV-1 in the Gauteng region, South Africa, using dried blood spots on filter papers

Seroepidemiology of HTLV-I in relation to that of HIV-1 in the Gauteng region, South Africa, using dried blood spots on filter papers

Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

Detection of HIV‐1 Infection in Dried Blood Spots from a 12‐Year‐Old ABO Bedside Test Card

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