Prevalence of Lawsonia intracellularis Infections in Pig Herds from the Western Romania

Pascu, C.; Costinar, L.; Mernea, Ioana; Tătar, Daniel; Herman, V.

doi:10.1016/j.aaspro.2015.08.100

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…From a medical point of view, these strains are of particular importance, because they are responsible for the appearance of the pyometra in sows, after insemination. Similar results were obtained by other researchers [ 4 , 7 , 18 , 19 , 20 , 21 , 22 ]. Ralstonia picketii and Achromobacter xylosoxidans can be found in the water distillation system of a boar stud facility that uses this water to expand raw semen.…”

Section: Discussionsupporting

confidence: 92%

Boar Semen Contamination: Identification of Gram-Negative Bacteria and Antimicrobial Resistance Profile

Costinar

Herman

Pitoiu³

et al. 2021

Animals

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Bacterial contamination of boar semen occurs with some frequency in artificial insemination centers and may have a negative effect on the quality of the semen as well as on the sows’ reproductive capacity. Normally, the source of bacterial contamination in pig seminal doses is the own boar. However, distilled water or laboratory equipment used to elaborate the seminal doses can be an important source of bacterial contamination. This study focused on the identification of gram-negative bacteria in boar semen, and impact on the quality of ejaculates obtained from boar, as well as on the establishment of antimicrobial resistance patterns of isolated gram-negative bacteria. Semen samples were collected from 96 boars, ranging in age from 12–36 month, from three artificial insemination centers from the North-West of Romania. Bacterial species were identified by two methods: matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and API 20 E (BioMerieux, France). The main bacteria isolated from the doses diluted semen were gram-negative bacteria (47.91%), with a majority of the contaminant bacteria belonging to the family Enterobacteriaceae: Seratia marcescens 19.56%, Proteus mirabilis 15.21% and Escherichia coli 10.86% and to the family Pseudomonaceae: Ralstonia picketii 17.39%, Burkholderia cepacia 10.86%, Pseudomonas aeruginosa 8.69%, and Pseudomonas fluorescens 4.34%, respectively. More than half of these isolates (56.52%) were resistant to gentamycin and 58.69% were resistant to penicillin. These antibiotics are very frequently added in sperm diluent in the centers for the processing of sperm from boars in Romania. Regular monitoring for bacterial contamination is an important aspect of a control program.

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Section: Discussionsupporting

confidence: 92%

Boar Semen Contamination: Identification of Gram-Negative Bacteria and Antimicrobial Resistance Profile

Costinar

Herman

Pitoiu³

et al. 2021

Animals

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“…In addition, our screening of fecal samples from both healthy pigs and/or diarrheal pigs demonstrated a higher positive rate in old pigs than in young pigs. This result agrees well with the report that L. intracellularis affects more frequently post-weaned pigs between 6 and 20 weeks of age ( 1 , 23 ). It is noteworthy that several published studies investigating the seroprevalence of L. intracellularis antibody among swine herds in both China and other countries have demonstrated a higher seropositivity in old pigs than in young pigs ( 16 , 24 ).…”

Section: Discussionsupporting

confidence: 93%

Fecal PCR survey and genome analysis of Lawsonia intracellularis in China

Wang,

Wu,

Zhao

et al. 2024

Front. Vet. Sci.

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Proliferative enteropathy caused by Lawsonia intracellularis is an important economic associated disease to pig industry, but the knowledge about the prevalence of L. intracellularis in pig farms in China is limited. In addition, there is no complete genome sequence available for L. intracellularis isolates from China. In this study, we developed a TaqMan qPCR for the screening of L. intracellularis by targeting the bacterial 16S rDNA gene. Laboratory evaluations revealed a good sensitivity and specificity on detecting L. intracellularis nucleic acid. Using this method, we investigated 891 fecal samples from apparently healthy pigs in 47 farms. The results demonstrated a screening positive rate of 37.3% (95% CI, 34.1–40.5%) for the samples, and a farm screening positive rate of 93.6% (95% CI, 65.3–94.4%). The screening positive rate at herd level ranged from 6.67% (95% CI, 0.2–31.9%) to 40% (95% CI, 38–79.6%), while at animal level, the highest screening positive rate was found in 12-week-old pigs [85.7% (95% CI, 67.3–96.0%)]. Investigation of 705 diarrheal or bloody feces from symptomatic pigs revealed that the highest positive rate was found in replacement gilts which was 37.18% (95% CI, 45.1–89.5%). Secondly, we conducted the complete genome sequence of a L. intracellularis PPE-GX01-2022 from China through PacBio sequencing. The genome of PPE-GX01-2022 consisted of a chromosome of 1,439,110 bp in length and three plasmids of 193,063, 39,799, and 27,067 bp, respectively. This genome encoded 1,428 predicted proteins, 44 tRNAs, and 6 rRNAs. Sequence comparisons demonstrated that the genome sequence of PPE-GX01-2022 was highly homologous to those of two isolates from US, and these three isolates shared 1,378 core genes. The screening results suggest a high prevalence rate of L. intracellularis in Chinese pig farms. In addition, the genome sequence of the Chinese isolate was highly homologous to those of the field isolates from the US.

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“…Clinical presentation is mainly characterized by high mortality associated with anaemia and haemorrhagic diarrhoea (Pascua et al 2015). However, some animals die without faecal abnormality and show only marked pallor (Vannucci et al 2019).…”

Section: Porcine Proliferative Enteropathymentioning

confidence: 99%

Sudden death associated with bleeding into digestive system of finishing pigs – a review

et al. 2021

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Sudden deaths of finishing pigs in modern pig herds cause economic losses and therefore draw constant attention worldwide. In the case of peracute mortality associated with gastrointestinal bleeding, pigs usually die during a short period without clinical manifestations. Necropsy can detect bleeding into various parts of the digestive system. Determining the exact aetiology of the sudden death can be difficult in many cases. Diseases and conditions such as gastric ulcers, abdominal torsion, haemorrhagic bowel syndrome and infectious diseases should be taken into account in the differential diagnosis. Because some of these diseases still have an unclear aetiology and pathogenesis, the aim of our work was to provide a summary of existing knowledge as well as to describe related pathognomonic pathological changes.

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Prevalence of Lawsonia intracellularis Infections in Pig Herds from the Western Romania

Cited by 3 publications

References 2 publications

Boar Semen Contamination: Identification of Gram-Negative Bacteria and Antimicrobial Resistance Profile

Boar Semen Contamination: Identification of Gram-Negative Bacteria and Antimicrobial Resistance Profile

Fecal PCR survey and genome analysis of Lawsonia intracellularis in China

Sudden death associated with bleeding into digestive system of finishing pigs – a review

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