Prevalence of infectious bronchitis and Newcastle disease virus among domestic and wild birds in H5N1 outbreaks areas

Abstract

Introduction: The first H5N1 outbreak in Burkina Faso was reported to the World Organization for Animal Health on 3 April 2006. This study aimed to determine the prevalence of avian influenza virus, infectious bronchitis virus, and Newcastle disease virus among domestic and wild birds in highly pathogenic avian influenza (HPAI) H5N1 outbreaks areas. Methodology: We collected paired tracheal and cloacal swabs from 283 birds including 278 domestic and five wild birds (three vultures, one sparrowhawk and one Western Grey Plantain-eater) in the Central Region (Ouagadougou) and the Western Region (Bobo-Dioulasso and Sokoroni) of Burkina Faso. Total RNA extracted from samples were subjected to reverse transcription and resulting cDNA amplified by PCR using specific primers for detection of Avian Influenza Virus (AIV mainly highly pathogenic H5N1), Infectious Bronchitis Virus (IBV), and Newcastle Disease Virus (NDV) for the first time in Burkina Faso. Results and conclusions: Our results show that 13.8% (39/283) samples were reactive for NDV, and the prevalence of IBV was 3.9% (11/283). None of the 283 birds were co-infected by AIV, IBV and/or NDV in our study areas. The prevalence of influenza A virus was 3.2% (95% CI: 0-6.6) with a 1.7% (95% CI: 0-3.2) prevalence of H5N1 being detected. Positive cases of H5N1 virus were found in two out of three vultures in Ouagadougou, and in three out of 203 local chickens in the Western Region. These results confirm the presence of influenza A H5N1 virus, IBV and NDV in domestic and wild birds in Burkina Faso.

New Avian Paramyxoviruses Type I Strains Identified in Africa Provide New Outcomes for Phylogeny Reconstruction and Genotype Classification

“…We included sequences in public databases representing all genotypes described so far and new isolates obtained and sequenced in this study in the framework of an international survey on wild and domestic birds in Africa. In this active surveillance survey, the prevalence of NDV in domestic birds was three times lower than that found in a study carried out in Burkina Faso [48]. In that study, the Burkina samples were also tested for the presence of influenza A viruses; the prevalence was estimated to be 3.2%.…”

Identification and Survey of a Novel Avian Coronavirus in Ducks

“…Two pairs of these primers and another pair of conserved primers reported previously [29], (Table 1) were successful in the amplification and sequencing of three regions of the viral 1b genes. The three regions were designated as 1b1 (848 bp), 1b2 (736 bp), and 1b3 (560 bp), corresponding to positions 15058–15921, 18164–18927, 14235–14756, respectively, in the genome (Figure 1).…”

Avian infectious bronchitis virus in Africa: a review

“…Between 1994 Two years later, in Burkina Faso, Tarnagda et al (2011) confirmed during a study on avian diseases [avian influenza virus (AIV), IBV and Newcastle disease (NCDV)] in domestic and wild birds in highly pathogenic avian influenza outbreaks areas that the prevalence of IBV was 3.9% and no co-infection by AIV, IBV, and/or NCDV was found.…”