SummaryAim: In recent years, the frequency of food allergy in children has gradually increased. Food allergy affects growth, development and quality of life adversely. There is no enough research in the preschool age group in which food allergy is observed more frequently. In this study, we investigated the prevalence of food allergy in nursery and kindergarten children living in Samsun. Material and Method: Nursery and kindergarten children living in Samsun were included in this study. A standardized self-administered questionnaire on food allergy was handed out to 2390 parents. We achieved a response rate of 53.5%. The parents of children with suspected food allergy were called by phone to learn detailed history. Results: The prevalence of food allergy in nursery and kindergarten children in Samsun province was found to be 7.7% (99 subjects). 51.5% of 99 subjects who were found to have food allergy were male. There were no differences between the mean age of children with FA (5.03±1.0) and the healthy children (5.17± 0.86). The prevalence of FA in the three-year-old group was lower than the other age groups. The most common allergic foods were hen's egg (25.3%) and chocolate (21.2%), foods additives (1.2%), strawberry (9.2%), cow milk (7.1%) and nut (4.1%). The most commonly reported systemic reactions against foods were related with skin (53.5%) and gastrointestinal systems (31.4%). Conclusions: The prevalence of FA was similar to our country and the Asian countries, but lower than the European and American countries. In preschool children with recurrent skin and gastrointestinal tract symptoms, FA should be kept in mind. (Turk Arch Ped 2013; 48: 288-293)