Prevalence of Vibrio
 spp. in raw shrimps (Parapenaeus longirostris
) and performance of a chromogenic medium for the isolation of Vibrio
 strains

Kriem, M.R.; Banni, B.; Bouchtaoui, H. El; Hamama, A.; Marrakchi, A. El; Chaouqy, N.; Robert-Pillot, Annick; Quilici, Marie‐Laure

doi:10.1111/lam.12455

Cited by 32 publications

(19 citation statements)

References 27 publications

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“…Four out of 5 (80%) V. parahaemolyticus isolates, representing 18.2% of the total Vibrios in the study, harboured tdh and trh virulence genes. This is quite high compared to other studies with lower detection rates [ 53 , 58 ] or the absence of these pathogenicity factors [ 52 ]. Nonetheless, detection rates were still lower compared to a study, whereby 31 out of 32 (96.8%) V. parahaemolyticus isolates from seafood possessed, at least, one ( n = 19) or both ( n = 13) virulent genes [ 10 ].…”

Section: Discussionmentioning

confidence: 62%

“…Similar observations have been reported in previous studies. In these studies, some or most of these Vibrio species were detected in fish and shrimps [ 51 ], shrimps only [ 52 ], aquatic samples [ 53 ], marine fish, and water [ 54 ]. The Vibrio species identified comprised thirteen isolates from borehole water, five from tap water, and four from dam water.…”

Section: Discussionmentioning

confidence: 99%

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Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

Maje

Tchatchouang

Manganyi

et al. 2020

International Journal of Microbiology

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The aim of this study was to characterise Vibrio species of water samples collected from taps, boreholes, and dams in the North West province, South Africa, and assess biocontrol potentials of their bacteriophages. Fifty-seven putative Vibrio isolates were obtained on thiosulfate-citrate-bile-salt-sucrose agar and identified using biochemical tests and species-specific PCRs. Isolates were further characterised based on the presence of virulence factors, susceptibility to eleven antibiotics, and biofilm formation potentials. Twenty-two (38.60%) isolates were confirmed as Vibrio species, comprising V. harveyi (45.5%, n = 10), V. parahaemolyticus (22.7%, n = 5), V. cholerae (13.6%, n = 3), V. mimicus (9.1%, n = 2), and V. vulnificus (9.1%, n = 2). Three of the six virulent genes screened were positively amplified; four V. parahaemolyticus possessed the tdh (18.18%) and trh (18.18%) genes, while the zot gene was harboured by 3 V. cholerae (13.64%) and one V. mimicus (4.55%) isolate. Isolates revealed high levels of resistance to cephalothin (95.45%), ampicillin (77.27%), and streptomycin (40.91%), while lower resistances (4.55%–27.27%) were recorded for other antimicrobials. Sixteen (72.7%) isolates displayed multiple antibiotic-resistant properties. Cluster analysis of antibiotic resistance revealed a closer relationship between Vibrio isolates from different sampling sites. The Vibrio species displayed biofilm formation potentials at 37°C (63.6, n = 14), 35°C (50%, n = 11), and 25°C (36.4%, n = 8). Two phages isolated in this study (vB_VpM_SA3V and vB_VcM_SA3V) were classified as belonging to the family Myoviridae based on electron microscopy. These were able to lyse multidrug-resistant V. parahaemolyticus and V. cholerae strains. These findings not only indicate the presence of antibiotic-resistant virulent Vibrio species from dam, borehole, and tap water samples that could pose a health risk to humans who either come in contact with or consume water but also present these lytic phages as alternative agents that can be exploited for biological control of these pathogenic strains.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

Maje

Tchatchouang

Manganyi

et al. 2020

International Journal of Microbiology

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“…Such anomalies are unlikely to be biological but rather due to technical and analytical differences, such as the choice of the 16S rRNA gene region sequenced (V4- vs. V3–V4-hypervariable region) and bioinformatic analysis settings. High abundance and prevalence of Vibrio and Photobacterium genera in shrimp guts suggest that they are more likely to be endogenous rather than pathogenic strains ( Kriem et al, 2015 ). However, this also reflects the persistence and adaptation of members from these genera to the shrimp gut environments and may potentially explain the susceptibility of shrimps to non-native pathogenic Vibrio and Photobacterium strains ( Kondo et al, 2014 ; Wang & Chen, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiome analysis of Pacific white shrimp gut and rearing water from Malaysia and Vietnam: implications for aquaculture research and management

Zoqratt

Eng

Thai³

et al. 2018

PeerJ

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Aquaculture production of the Pacific white shrimp is the largest in the world for crustacean species. Crucial to the sustainable global production of this important seafood species is a fundamental understanding of the shrimp gut microbiota and its relationship to the microbial ecology of shrimp pond. This is especially true, given the recently recognized role of beneficial microbes in promoting shrimp nutrient intake and in conferring resistance against pathogens. Unfortunately, aquaculture-related microbiome studies are scarce in Southeast Asia countries despite the severe impact of early mortality syndrome outbreaks on shrimp production in the region. In this study, we employed the 16S rRNA amplicon (V3–V4 region) sequencing and amplicon sequence variants (ASV) method to investigate the microbial diversity of shrimp guts and pond water samples collected from aquaculture farms located in Malaysia and Vietnam. Substantial differences in the pond microbiota were observed between countries with the presence and absence of several taxa extending to the family level. Microbial diversity of the shrimp gut was found to be generally lower than that of the pond environments with a few ubiquitous genera representing a majority of the shrimp gut microbial diversity such as Vibrio and Photobacterium, indicating host-specific selection of microbial species. Given the high sequence conservation of the 16S rRNA gene, we assessed its veracity at distinguishing Vibrio species based on nucleotide alignment against type strain reference sequences and demonstrated the utility of ASV approach in uncovering a wider diversity of Vibrio species compared to the conventional OTU clustering approach.

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“…Otherwise, it will be less effective and more expensive eradicating the undesired bacterial from the aquatic organism. Besides, this method could be done with others chromogenic agar, like those used to monitor other bacteria associated to foodborne disease (Tavakoli et al, 2008) or to monitor pathogens that damages aquatic organisms (Kriem et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture

Strahsburger¹,

Retamales²,

Estrada³

et al. 2016

lajar

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ABSTRACT.The microdot method is a downscaling methodology of traditional tenfold serial dilution procedure used in microbiology. The microdot method uses 100 µL for serial dilution and count colonies in a spot of 10 µL. In this study we counted colonies directly in a chromogenic agar plate to determine, at the same time, the presence and cell concentration of target bacteria required for sanitary monitoring of Chilean export fishery products. Due to among importers countries the most concerning bacteria included in sanitary monitoring are Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, we used the chromogenic agar; CHROMagar ECC, CHROMagar Listeria and Baird Parker agar, respectively. The results shows no differences between quantitative results obtained with microdot and traditional method during the quantification of a culture of Escherichia coli (1.5 L). The sensitivity and specificity of the microdot method in association with each chromogenic agar was demonstrated in vitro with reference strains. In addition, the usefulness in sanitary monitoring of aquaculture procedures was evaluated in Artemia salina tanks. This method did not detected sanitary problems in surface water. Although other colonies grown in the chromogenic agar plate, their morphological and chromogenic properties not correspond to Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, being identified as Salmonella enterica subsp. enterica, Microbacterium sp., Bacillus sp. and Staphylococcus pasteuri by 16S rRNA gene sequence analysis. Hence, we propose the microdot chromogenic method as a low cost, specific and reliable procedure for sanitary monitoring of aquaculture procedures. Keywords: microdot method, sanitary monitoring, aquaculture, chromogenic agar.Método de la microgota: usado con agar cromogénico es un procedimiento útil para el monitoreo sanitario en acuicultura RESUMEN. El método de la microgota es una versión a menor escala del procedimiento tradicional de dilución en serie en base diez utilizados en microbiología. El método realiza diluciones en 100 µL y cuenta colonias crecidas en una gota de 10 µL. En este estudio se cuentan colonias directamente en placas cromogénicas para determinar densidad celular y presencia de bacterias requeridas en vigilancia sanitaria de productos pesqueros chilenos de exportación. Entre los requisitos de países importadores, la vigilancia sanitaria involucra frecuentemente a Escherichia coli, Listeria monocytogenes y Staphylococcus aureus, por lo que se utilizan los agares cromogénicos; CHROMagar ECC, CHROMagar Listeria y agar Baird Parker para su identificación. La comparación entre el método de microgota y el método tradicional no muestra diferencias al evaluar un cultivo de Escherichia coli (1,5 L). La sensibilidad y especificidad del método de microgota junto a cada agar cromogénico se demostró in vitro con cepas de referencia. Además, en estanques de Artemia salina se evaluó la utilidad de este método para el monitoreo sanitario. Este método no mostró problema...

show abstract

Prevalence of Vibrio spp. in raw shrimps (Parapenaeus longirostris ) and performance of a chromogenic medium for the isolation of Vibrio strains

Cited by 32 publications

References 27 publications

Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

Microbiome analysis of Pacific white shrimp gut and rearing water from Malaysia and Vietnam: implications for aquaculture research and management

Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture

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