Prevalence of <i>netB-</i>positive <i>Clostridium perfringens</i> in Italian poultry flocks by environmental sampling

Profeta, Francesca; Francesco, Cristina Esmeralda Di; Provvido, Andrea Di; Scacchia, Massimo; Alessiani, Alessandra; Giannatale, Elisabetta Di; Marruchella, Giuseppe; Orsini, Massimiliano; Toscani, T; Marsilio, Fulvio

doi:10.1177/1040638719885841

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…The phylogenetic analysis of the netB gene of the present C. perfringens isolate (MT032252) with the ten selected and published sequences of C. perfringens isolates from difference countries showed an extremely high sequence identity, and the functionally critical motifs, structures and amino acids for the toxic activity in the NetB toxin were absolutely conserved. Similar results were reported by Profeta et al (2020), these authors analysed 960 sequences of the netB genes of strains isolated from poultry in Italy and found a strong conservation in the netB gene sequences in their countries and in other countries. We further checked twenty other netB gene sequences available in the GenBank in order to figure out which mutated points occurred in the netB genes of the C. perfringens isolated from the Vietnamese chickens.…”

Section: Discussionsupporting

confidence: 84%

Characterisation of Clostridium perfringens isolated from chickens in Vietnam

Thi¹,

Vu-Khac²,

Duc³

2021

Vet. Med. - Czech

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The objective of this study was isolating and characterising Clostridium perfringens from chickens in Vietnam and identifying virulence factors involved with enteritis. Five hundred thirty-one faecal and sixty-eight intestinal samples were collected from healthy and diseased chickens for the C. perfringens isolation. The presence of virulence factors was determined by multiplex PCR. The netB gene of the selected isolates was sequenced and checked for its expression by SDS-PAGE. Two hundred seventy-two C. perfringens isolates were collected. All of them were shown to be positive for the cpa gene. The netB gene was detected in 26.56% of the C. perfringens isolates from the healthy chickens, while 43.45% of the isolates from the faeces and 45% of the isolates from the intestinal samples were positive for this gene in the diseased birds. All eight isolates positive to netB from the diseased chickens showed 100% identity in the netB sequence and produced the NetB toxin in vitro, whereas only two out of eight healthy chicken-derived isolates produced this toxin. Nine out of ten chickens experimentally infected with the C. perfringens netB-positive isolate showed typical signs of enteritis. The cpa gene was the most prevalent virulence factor identified in the bacteria C. perfringens, but the netB gene could be a major player responsible for necrotic enteritis progression in chickens.

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Section: Discussionsupporting

confidence: 84%

Characterisation of Clostridium perfringens isolated from chickens in Vietnam

Thi¹,

Vu-Khac²,

Duc³

2021

Vet. Med. - Czech

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“…The molecular-based detection of netB-positive C. perfringens isolates in NE-afflicted broiler flocks has been reported over the last decade (Table 3) [59][60][61][62][63][64][65]. A summary of the most frequently studied virulent NE-causing strains globally highlights the importance of netB + C. perfringens for experimentally reproducible NE models (Table 3).…”

Section: Perfringens Netb Toxin and Its Role In Nementioning

confidence: 99%

Role of Clostridium perfringens Necrotic Enteritis B-like Toxin in Disease Pathogenesis

Lee

Lillehoj

2021

Vaccines

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Necrotic enteritis (NE) is a devastating enteric disease caused by Clostridium perfringens type A/G that impacts the global poultry industry by compromising the performance, health, and welfare of chickens. Coccidiosis is a major contributing factor to NE. Although NE pathogenesis was believed to be facilitated by α-toxin, a chromosome-encoded phospholipase C enzyme, recent studies have indicated that NE B-like (NetB) toxin, a plasmid-encoded pore-forming heptameric protein, is the primary virulence factor. Since the discovery of NetB toxin, the occurrence of NetB+ C. perfringens strains has been increasingly reported in NE-afflicted poultry flocks globally. It is generally accepted that NetB toxin is the primary virulent factor in NE pathogenesis although scientific evidence is emerging that suggests other toxins contribute to NE. Because of the complex nature of the host-pathogen interaction in NE pathogenesis, the interaction of NetB with other potential virulent factors of C. perfringens needs better characterization. This short review will summarize the primary virulence factors involved in NE pathogenesis with an emphasis on NetB toxin, and a new detection method for large-scale field screening of NetB toxin in biological samples from NE-afflicted commercial broiler flocks.

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“…The production losses due to NE outbreaks in the global poultry industry are estimated to be US $6 billion annually (Moore, 2016). The main sources of NE infection are litter and contami-nated environment (Craven et al, 2003;Profeta et al, 2020), and transmission of infection occurs through ingestion of contaminated food and water. Husbandry practices like diet and litter types influence the incidence and severity of NE in poultry (Henry et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis

Eraky¹,

El-Ghany

2021

J. Indonesian Trop. Anim. Agric.

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The aims of this investigation were characterization, demonstration of the antibiogram pattern and detection of the pathogenicity of Clostridium perfringens (C. perfringens) strains isolated from broiler chickens in Damietta governorate, Egypt. A total of 357 samples representing 202 intestinal contents and 155 liver samples from freshly dead broiler chickens were collected from 18 broiler farms. Isolates of C. perfringens were identified morphologically, microscopically, and biochemically. Forty-seven C. perfringens isolates were recovered, which represented 20.3% of the intestinal contents and 3.8% of the liver samples. The toxins and virulence genes of C. perfringens were investigated using polymerase chain reaction. All of the toxigenic C. perfringens strains were type A and carried netB, tpeL, cpe, and plc genes. The in vitro antibiogram of C. perfringens strains revealed 100% sensitivity to gentamycin and levofloxacin and 100% resistance to nalidixic acid and ceftriaxone. The isolated C. perfringens strains were highly pathogenic and induced signs and lesions of necrotic enteritis as well as 43.3% mortalities in 20-day-old chicks. In conclusion, C. perfringens is an important pathogen that affects broiler chickens due to the presence of virulence genes and the pathogenicity in the inoculated birds.

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Prevalence of netB-positive Clostridium perfringens in Italian poultry flocks by environmental sampling

Cited by 10 publications

References 20 publications

Characterisation of Clostridium perfringens isolated from chickens in Vietnam

Characterisation of Clostridium perfringens isolated from chickens in Vietnam

Role of Clostridium perfringens Necrotic Enteritis B-like Toxin in Disease Pathogenesis

Genetic characterization, antibiogram pattern, and pathogenicity of Clostridium perfringens isolated from broiler chickens with necrotic enteritis

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